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The map label for this gene is emrA [H]

Identifier: 119355944

GI number: 119355944

Start: 109147

End: 110133

Strand: Reverse

Name: emrA [H]

Synonym: Cpha266_0094

Alternate gene names: 119355944

Gene position: 110133-109147 (Counterclockwise)

Preceding gene: 119355945

Following gene: 119355943

Centisome position: 3.51

GC content: 56.74

Gene sequence:

>987_bases
ATGGCCGAACAGGAAAAGAACATCGGAACCGCAGAAAAACCGGAAGAAAAAGCAACCAACCCGCTGCGTCTTGTCATCAC
AGTCCTGCTCGTTCTGGCTGCGGTCGTCTGGGGAGGAAGCAAAATCCGGCACTCTTTCCTCTACGTCGAAACCGATAACG
CCCAGATCGAAGGGGACGTCTACCCTGTCATCTCAAAGGTGCCGGGAACAGTACAGAGCGTCCTTGTAAGAACAAATCAG
GAGGTGCGGAGAGGCGATACCCTTGTGCTGCTTGATCCGGCGGATTACAAAGTCAAACGGGATATGGCTGAAGCCGCACT
GCAGAATGCCCGCGCATCTGTTTCAGCTGCTGGCGAAGCCGCAACAGCAGCCGCGGCAACCGGCCAGAAGCTCAAATCCG
ATCTCGCTCGAAGTCAGAACCTTCGTCGCCAGGATGTTATTTCGCCCTCGGAATTTGACGCCGTAAAAGCCGCTGCTCTC
TCTTCATCGGCACAATCTGCCGCAGCGGAAAGCCAGCACCGAGGCGCCGCTGCGCAGGTAACCATGCGCCAGGCCGAACT
GCGCAACGCCGACCTGCAGCTCTCCTATACCACGATCACCGCCCCTGCAGCGGGACATGTGTCGAAAAAGAATGTGCAGC
CAGGACAATATGTAGCACCCGGCCAGCAGCTCATCGCGCTGGTCGGCAGCAGCGAACTCTGGGTTGTAGCGAACTTCAAG
GAAACGCAGCTCGAAAACATCCGTACCGGCCAACCTGTCACCATCAGGGTGGATGCCTATCCGGGAAAAGAGTTTGAAGG
CCGGGTGGAATCGATCTCCTCGGGAACCGGAGCGCGCTTCGCCCTGCTTCCTCCCGACAATGCCAGCGGCAACTTCGTCA
AGGTAGCCCAGCGCGTTCCGGTCAAGATTCTGTTTACCGGCAAACCCGACAAGGATCACAAACTTGCCGCAGGCATGAAC
GTTGTTGTTGAGATAAAAGTAAAGTGA

Upstream 100 bases:

>100_bases
CCGGTAGCCATCTATAAAGTCCCCGACAGAAGAACTCCCGCAAGGAAACGGCAGGAAGCCACAACACTTCTTTTAAAAGA
CAACAAACAAACAGCATAAC

Downstream 100 bases:

>100_bases
CCTGGCCAGAGGGGCTATGTCATCCCGAACTGAAAGAAAGGATCGCCGGAATTCGAAATGACTAATTACAAACATACAGG
AATGTGTCAACAGCATAGCG

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAEQEKNIGTAEKPEEKATNPLRLVITVLLVLAAVVWGGSKIRHSFLYVETDNAQIEGDVYPVISKVPGTVQSVLVRTNQ
EVRRGDTLVLLDPADYKVKRDMAEAALQNARASVSAAGEAATAAAATGQKLKSDLARSQNLRRQDVISPSEFDAVKAAAL
SSSAQSAAAESQHRGAAAQVTMRQAELRNADLQLSYTTITAPAAGHVSKKNVQPGQYVAPGQQLIALVGSSELWVVANFK
ETQLENIRTGQPVTIRVDAYPGKEFEGRVESISSGTGARFALLPPDNASGNFVKVAQRVPVKILFTGKPDKDHKLAAGMN
VVVEIKVK

Sequences:

>Translated_328_residues
MAEQEKNIGTAEKPEEKATNPLRLVITVLLVLAAVVWGGSKIRHSFLYVETDNAQIEGDVYPVISKVPGTVQSVLVRTNQ
EVRRGDTLVLLDPADYKVKRDMAEAALQNARASVSAAGEAATAAAATGQKLKSDLARSQNLRRQDVISPSEFDAVKAAAL
SSSAQSAAAESQHRGAAAQVTMRQAELRNADLQLSYTTITAPAAGHVSKKNVQPGQYVAPGQQLIALVGSSELWVVANFK
ETQLENIRTGQPVTIRVDAYPGKEFEGRVESISSGTGARFALLPPDNASGNFVKVAQRVPVKILFTGKPDKDHKLAAGMN
VVVEIKVK
>Mature_327_residues
AEQEKNIGTAEKPEEKATNPLRLVITVLLVLAAVVWGGSKIRHSFLYVETDNAQIEGDVYPVISKVPGTVQSVLVRTNQE
VRRGDTLVLLDPADYKVKRDMAEAALQNARASVSAAGEAATAAAATGQKLKSDLARSQNLRRQDVISPSEFDAVKAAALS
SSAQSAAAESQHRGAAAQVTMRQAELRNADLQLSYTTITAPAAGHVSKKNVQPGQYVAPGQQLIALVGSSELWVVANFKE
TQLENIRTGQPVTIRVDAYPGKEFEGRVESISSGTGARFALLPPDNASGNFVKVAQRVPVKILFTGKPDKDHKLAAGMNV
VVEIKVK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=320, Percent_Identity=33.75, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1788711, Length=300, Percent_Identity=35.6666666666667, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790519, Length=292, Percent_Identity=32.5342465753425, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081951, Length=309, Percent_Identity=26.537216828479, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790024, Length=233, Percent_Identity=26.6094420600858, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789637, Length=321, Percent_Identity=28.0373831775701, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790012, Length=234, Percent_Identity=25.6410256410256, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 35016; Mature: 34885

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQEKNIGTAEKPEEKATNPLRLVITVLLVLAAVVWGGSKIRHSFLYVETDNAQIEGDV
CCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCEECCCE
YPVISKVPGTVQSVLVRTNQEVRRGDTLVLLDPADYKVKRDMAEAALQNARASVSAAGEA
EEHHHCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCH
ATAAAATGQKLKSDLARSQNLRRQDVISPSEFDAVKAAALSSSAQSAAAESQHRGAAAQV
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TMRQAELRNADLQLSYTTITAPAAGHVSKKNVQPGQYVAPGQQLIALVGSSELWVVANFK
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCHHHEEEEECCCCEEEEECCC
ETQLENIRTGQPVTIRVDAYPGKEFEGRVESISSGTGARFALLPPDNASGNFVKVAQRVP
HHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
VKILFTGKPDKDHKLAAGMNVVVEIKVK
EEEEECCCCCCCCEEECCCEEEEEEEEC
>Mature Secondary Structure 
AEQEKNIGTAEKPEEKATNPLRLVITVLLVLAAVVWGGSKIRHSFLYVETDNAQIEGDV
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEEECCCCEECCCE
YPVISKVPGTVQSVLVRTNQEVRRGDTLVLLDPADYKVKRDMAEAALQNARASVSAAGEA
EEHHHCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCH
ATAAAATGQKLKSDLARSQNLRRQDVISPSEFDAVKAAALSSSAQSAAAESQHRGAAAQV
HHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TMRQAELRNADLQLSYTTITAPAAGHVSKKNVQPGQYVAPGQQLIALVGSSELWVVANFK
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCEECCHHHEEEEECCCCEEEEECCC
ETQLENIRTGQPVTIRVDAYPGKEFEGRVESISSGTGARFALLPPDNASGNFVKVAQRVP
HHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCC
VKILFTGKPDKDHKLAAGMNVVVEIKVK
EEEEECCCCCCCCEEECCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]