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The map label for this gene is argF

Identifier: 118581573

GI number: 118581573

Start: 3485437

End: 3486354

Strand: Reverse

Name: argF

Synonym: Ppro_3172

Alternate gene names: 118581573

Gene position: 3486354-3485437 (Counterclockwise)

Preceding gene: 118581574

Following gene: 118581572

Centisome position: 86.98

GC content: 64.92

Gene sequence:

>918_bases
ATGGCGCGACACTTTCTCTCGCTGCACGACTACACCAAGGAAGAACTGGACCGGCTGCTGGCGCTGGCCATCGAGCTTAA
GCAGGAGCAGAAGCAGGGGGGCGAACATCATCTGCTCAAGGGCAAGACCCTGGCCATGATCTTCGACAAATCATCCACCC
GCACCCGCATCTCCTTCGAGGTCGGCATCTACCAGCTGGGGGGGCACGGTCTATTCATCTCCACCGGCACATCCCAGATG
GGGCGCGGCGAACCGCTCAAGGACACTGCCCGCGTGCTCTCCCGCTACTGCGACGGCATCATGATCCGCACCTTCGGGCA
GGAGATCGTGGACGAGCTGGCCGCCTACGCCACGGTGCCGGTGATCAACGGCCTGACCGACCTGTTCCACCCCTGCCAGA
TCATGGCCGACGTGATGACGGTCATGGAGCATAAGGGGGGCTACGCCGGGCTGAAGTTCGCCTGGGTCGGCGACGGCAAC
AACATGGCCAACACCTGGATCGAGGCTGCCGCCATCTTCGGCTTCGACCTGGCCCTTGCCTGCCCGCAGGGGTACGAGCC
GGATGCCAAGGTCATGGCCTGGGCCGCGGAGCGGGCGCCCGGCAGGGTTACCCTCACGGCTGACCCCAAAGAGGCGGTGA
GCGGTGCCCATGTGATCAACACCGACGTCTGGGCCAGCATGGGCATGGAGGCCGAGCAGAAGGTGCGCGAGCAGGCCTTC
GGCGGCTTTTGCCTTGACGACGCACTGGCAGGGCTGGCCCATCCGGACCACATCGTACTGCACTGCCTGCCCGCCCATCG
CGGCGAGGAAATTTCCGATTCGGTCATCGAGGGGTCCAACTCCCGGGTCTGGGACGAGGCCGAGAACCGGCTGCACGCTC
AGAAGGCGATCATGGCCACCCTGATGGGCGGAGAGTAG

Upstream 100 bases:

>100_bases
TCGTGCCGCCGCTGATCGTAACCAAACAGGAAGTTGATCAGATGATTTTCATTCTCGACGGCATCTTGGCCGAGGTCGAG
GCATAACAAGGGGGTGCACT

Downstream 100 bases:

>100_bases
GGCCTTTCCCGTGCCCCTCCTCCCCTGGGGAGAGAACCGGCCGCGGCAGGGCTTTGCGACACATTCAACATGGTAAGGAG
AAGCGCATGACACAGCAGAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFEVGIYQLGGHGLFISTGTSQM
GRGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVPVINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGN
NMANTWIEAAAIFGFDLALACPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAF
GGFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMATLMGGE

Sequences:

>Translated_305_residues
MARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFEVGIYQLGGHGLFISTGTSQM
GRGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVPVINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGN
NMANTWIEAAAIFGFDLALACPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAF
GGFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMATLMGGE
>Mature_304_residues
ARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFEVGIYQLGGHGLFISTGTSQMG
RGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVPVINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGNN
MANTWIEAAAIFGFDLALACPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAFG
GFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMATLMGGE

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=45.3947368421053, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=27.8846153846154, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=44.0366972477064, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1786469, Length=328, Percent_Identity=42.6829268292683, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=29.153605015674, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI48994908, Length=346, Percent_Identity=23.6994219653179, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=163, Percent_Identity=33.7423312883436, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=35.5987055016181, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=25.8899676375405, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=27.0700636942675, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_PELPD (A1ATU5)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902823.1
- ProteinModelPortal:   A1ATU5
- SMR:   A1ATU5
- STRING:   A1ATU5
- GeneID:   4573274
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3172
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   QSEREEV
- PhylomeDB:   A1ATU5
- ProtClustDB:   PRK00779
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33325; Mature: 33194

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 103-103 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFE
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEE
VGIYQLGGHGLFISTGTSQMGRGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVP
EEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHH
VINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGNNMANTWIEAAAIFGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAF
CCCCCCCCHHHEEEHHHCCCCEEEEECCHHHHHCCCEEEEHHHHHHCCCCHHHHHHHHHC
GGFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMAT
CCEEHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LMGGE
HCCCC
>Mature Secondary Structure 
ARHFLSLHDYTKEELDRLLALAIELKQEQKQGGEHHLLKGKTLAMIFDKSSTRTRISFE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEE
VGIYQLGGHGLFISTGTSQMGRGEPLKDTARVLSRYCDGIMIRTFGQEIVDELAAYATVP
EEEEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHH
VINGLTDLFHPCQIMADVMTVMEHKGGYAGLKFAWVGDGNNMANTWIEAAAIFGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPQGYEPDAKVMAWAAERAPGRVTLTADPKEAVSGAHVINTDVWASMGMEAEQKVREQAF
CCCCCCCCHHHEEEHHHCCCCEEEEECCHHHHHCCCEEEEHHHHHHCCCCHHHHHHHHHC
GGFCLDDALAGLAHPDHIVLHCLPAHRGEEISDSVIEGSNSRVWDEAENRLHAQKAIMAT
CCEEHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LMGGE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA