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The map label for this gene is argG

Identifier: 118581572

GI number: 118581572

Start: 3484124

End: 3485350

Strand: Reverse

Name: argG

Synonym: Ppro_3171

Alternate gene names: 118581572

Gene position: 3485350-3484124 (Counterclockwise)

Preceding gene: 118581573

Following gene: 118581571

Centisome position: 86.96

GC content: 62.84

Gene sequence:

>1227_bases
ATGACACAGCAGAAAATGGACATCAAAAACGTCGTCCTGGCATATTCGGGCGGTTTGGATACCTCGATCATCCTTAAGTG
GCTCAAGAACGAGTACGGCTGCAGGGTAGTGGCCTTTTCCGCCGACCTGGGGCAGGGGGAGGAGCTGGACCCGGTGCGCG
AAAAGGCACTGGCCACCGGTGCCGACGTGGTCTACATCGACGACCTGCGCGAGGAGTTCGTGCGCGACTTCGTCTTTCCC
ATGTTCCGCGCCAACGCCATCTACGAGGGGCACTACCTGCTGGGCACCTCCATCGCCCGGCCGCTGATCGCCAAGCGCCA
GATGGAGATCGCCGCCAAAGAGGGGTGTGACGCGGTCTCCCACGGCTCCACCGGCAAGGGCAACGACCAGGTCCGCTTCG
AGCTGGGCTACTACCACTTCAATCCCAACATCAAGATCGTGGCCCCCTGGCGCACCTGGGACCTGAACAGCCGCCAGGCC
CTGATCGACTACGCCAAGAAGAACGGCATTCCGGTGCCGGTCACCAAGAAGCGCCCCTGGTCCTCGGACCGCAACCTGCT
GCACATCTCCTTCGAAGGGGGCATCCTGGAGGATACCTGGGCCGAGGCGCCCGAGGAGATGTACGTGCTGACCACGGCTC
CGGAAAAGGCGCCCAACAAGCCGCAGTACGTGGAGATCGAGTTCAAGAACGGCAACGCCGTTGCCGTGGATGGCGAGAAG
ATGACCCCGGCCCAGCTTTTGGCCCACCTCAACTACCTGGGTGGACAGCACGGCATTGGCCGGGTGGACCTGCTGGAGAA
CCGCTCGGTGGGCATGAAGTCCCGCGGTGTGTACGAGACGCCGGGCGGCACCATCCTGCGCGAGGCCCACATGGCGGTGG
AGCAGATCACCATGGACCGCGAGGTCATGCGCATCCGCGACGGCCTGATCCCCGAGTACGCCCGCCTCGTCTATGCCGGC
TACTGGTTTTCGCCGGAGCGCGAGATGCTGCAGGCGCTGATCGACGATTCCCAGAAGTGCGTCAACGGCGTGGCCCGCCT
GAAACTCTACAAGGGATACTGCCGCACCGTGGGGCGCAAGTCCGATACTGACTCGCTCTTCAACCAGGATTTCGCCACCT
TCGAGAAGGACCAGGTCTACAACCAGGCCGACGCCGAAGGGTTCATCAGGATCAATTCGCTCCGCCTGCGCATCAGGTCC
ATGATGCAGGCGGCCAAGAAGAAGTGA

Upstream 100 bases:

>100_bases
TGGGCGGAGAGTAGGGCCTTTCCCGTGCCCCTCCTCCCCTGGGGAGAGAACCGGCCGCGGCAGGGCTTTGCGACACATTC
AACATGGTAAGGAGAAGCGC

Downstream 100 bases:

>100_bases
GGAGTGAGAGGTACAGAGTAAGGTGATCCTGCTGTCGAGGGGGAGGTCTCTGCGCTCCCCCTCGCGGCAGGCCTGTGGGA
TATGAGGGGAATGGGCAGGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTQQKMDIKNVVLAYSGGLDTSIILKWLKNEYGCRVVAFSADLGQGEELDPVREKALATGADVVYIDDLREEFVRDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAAKEGCDAVSHGSTGKGNDQVRFELGYYHFNPNIKIVAPWRTWDLNSRQA
LIDYAKKNGIPVPVTKKRPWSSDRNLLHISFEGGILEDTWAEAPEEMYVLTTAPEKAPNKPQYVEIEFKNGNAVAVDGEK
MTPAQLLAHLNYLGGQHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMRIRDGLIPEYARLVYAG
YWFSPEREMLQALIDDSQKCVNGVARLKLYKGYCRTVGRKSDTDSLFNQDFATFEKDQVYNQADAEGFIRINSLRLRIRS
MMQAAKKK

Sequences:

>Translated_408_residues
MTQQKMDIKNVVLAYSGGLDTSIILKWLKNEYGCRVVAFSADLGQGEELDPVREKALATGADVVYIDDLREEFVRDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAAKEGCDAVSHGSTGKGNDQVRFELGYYHFNPNIKIVAPWRTWDLNSRQA
LIDYAKKNGIPVPVTKKRPWSSDRNLLHISFEGGILEDTWAEAPEEMYVLTTAPEKAPNKPQYVEIEFKNGNAVAVDGEK
MTPAQLLAHLNYLGGQHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMRIRDGLIPEYARLVYAG
YWFSPEREMLQALIDDSQKCVNGVARLKLYKGYCRTVGRKSDTDSLFNQDFATFEKDQVYNQADAEGFIRINSLRLRIRS
MMQAAKKK
>Mature_407_residues
TQQKMDIKNVVLAYSGGLDTSIILKWLKNEYGCRVVAFSADLGQGEELDPVREKALATGADVVYIDDLREEFVRDFVFPM
FRANAIYEGHYLLGTSIARPLIAKRQMEIAAKEGCDAVSHGSTGKGNDQVRFELGYYHFNPNIKIVAPWRTWDLNSRQAL
IDYAKKNGIPVPVTKKRPWSSDRNLLHISFEGGILEDTWAEAPEEMYVLTTAPEKAPNKPQYVEIEFKNGNAVAVDGEKM
TPAQLLAHLNYLGGQHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDREVMRIRDGLIPEYARLVYAGY
WFSPEREMLQALIDDSQKCVNGVARLKLYKGYCRTVGRKSDTDSLFNQDFATFEKDQVYNQADAEGFIRINSLRLRIRSM
MQAAKKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=50.2475247524753, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=50.2475247524753, Blast_Score=392, Evalue=1e-109,
Organism=Escherichia coli, GI1789563, Length=410, Percent_Identity=26.0975609756098, Blast_Score=121, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=43.5960591133005, Blast_Score=356, Evalue=4e-99,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=45.049504950495, Blast_Score=343, Evalue=9e-95,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PELPD (A1ATU4)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_902822.1
- ProteinModelPortal:   A1ATU4
- SMR:   A1ATU4
- STRING:   A1ATU4
- GeneID:   4573273
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_3171
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   A1ATU4
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 46135; Mature: 46004

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 41-41 BINDING 92-92 BINDING 97-97 BINDING 122-122 BINDING 124-124 BINDING 128-128 BINDING 128-128 BINDING 129-129 BINDING 132-132 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQKMDIKNVVLAYSGGLDTSIILKWLKNEYGCRVVAFSADLGQGEELDPVREKALATG
CCCCHHCHHHEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
ADVVYIDDLREEFVRDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAAKEGCDAVS
CCEEEEHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
HGSTGKGNDQVRFELGYYHFNPNIKIVAPWRTWDLNSRQALIDYAKKNGIPVPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCCEEEEECCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
SSDRNLLHISFEGGILEDTWAEAPEEMYVLTTAPEKAPNKPQYVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCC
MTPAQLLAHLNYLGGQHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDR
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDGLIPEYARLVYAGYWFSPEREMLQALIDDSQKCVNGVARLKLYKGYCRTVGRK
HHHHHHHCCCHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
SDTDSLFNQDFATFEKDQVYNQADAEGFIRINSLRLRIRSMMQAAKKK
CCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQKMDIKNVVLAYSGGLDTSIILKWLKNEYGCRVVAFSADLGQGEELDPVREKALATG
CCCHHCHHHEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
ADVVYIDDLREEFVRDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAAKEGCDAVS
CCEEEEHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
HGSTGKGNDQVRFELGYYHFNPNIKIVAPWRTWDLNSRQALIDYAKKNGIPVPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCCEEEEECCEECCCCCCHHHHHHHHHCCCCCCCCCCCCC
SSDRNLLHISFEGGILEDTWAEAPEEMYVLTTAPEKAPNKPQYVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEECCCC
MTPAQLLAHLNYLGGQHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHMAVEQITMDR
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDGLIPEYARLVYAGYWFSPEREMLQALIDDSQKCVNGVARLKLYKGYCRTVGRK
HHHHHHHCCCHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
SDTDSLFNQDFATFEKDQVYNQADAEGFIRINSLRLRIRSMMQAAKKK
CCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA