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The map label for this gene is atoS [H]

Identifier: 118581481

GI number: 118581481

Start: 3366744

End: 3367853

Strand: Direct

Name: atoS [H]

Synonym: Ppro_3078

Alternate gene names: 118581481

Gene position: 3366744-3367853 (Clockwise)

Preceding gene: 118581480

Following gene: 118581482

Centisome position: 84.0

GC content: 62.79

Gene sequence:

>1110_bases
ATGAACCTACCTGACTCACGAGAAGAAAACCAGATTCCGCTGGATGACTACTACGCCACGGTCATCGACAGCGTCGGCGA
CGGCGTGCTGGTCATCAACCGCGAGGGGCTGATCACGCTCTGCAATCCGGCTGCCGAGGAGATCACCGGCCTCTCCCGGC
GCCACGCCCTGGGCGCCAGCTTCCGGAAGCTCTTCTCCCATGAGGCCACGCTCCTGGAGATGGTCAACAAGACCACCCGC
ACCGGCGTCACCATATCCGACCATGAAAACGTCGTCGTGCGCGGCATGGCCAGGGTCACCCCGGTGGCGGTCACCTGCTA
CCCCCTGATGCGCGCCAACGGCGAGAACATCGGCGCCATCCTGACCCTCAAGGACATCACCTATATCCGCGAACTGGAGG
CGGCGGTGCGCCAGGCGGACCGCCTCTCCACTTTGGGCACCCTGGCCGCCGGACTGGCCCATGAGGTAAAGAACCCCCTG
GGCGGCATCAAGGGGGCGGCCCAACTGCTGGAGCAGGAACTGGCCGCGGAGAGCGAACTTCTGGAGTACACCCGGGTGAT
GATCCGTGAAACGGAGCGCATCGACCGTATCATCAGGGAACTCCTGGAACTTGCCTCGCCACGGGGCCTGAAGCTGGCTC
CAGTCAACCTGCACCGCATACTGGGGGACATCCTGCTGCTGCAGAAGCAGGCGGTGGCGGATCGCCAGATCTCATTCGTG
ACCCATTTCGATCCCAGCATCCCCGACATCATGGCCGACAGAGAGATGCTGACCAGGCTGTTTCTGAACCTGATCCGCAA
CGCCATCGACGCCATTGCCGAATCCGGGCAACTGACCGTGACCAGCCGCGTCCTGTCCGACTACCGTCTGGCCCAGAACC
AACGGCGTTCACGCATGGTTGCGGTGGATATCGCCGACGATGGCCCCGGCATTCCCGGCGCTGACCTGGAAAACATCTGG
ACCCCCTTTTTCAGCAGCAAATCCACCGGCACCGGACTGGGGCTTACCATCTGCCACAAGATCGTGTCGGAACATCGCGG
CATGATCAAGGTGGAGTCAGATCCGGGCCACGGCACCAAGTTCACGGTTCTGCTGCCGCTGGTGCAGTAG

Upstream 100 bases:

>100_bases
GCCGGCGCGGCGGACGTACGCCGGGAGGCCAACAACGCACGCTCGACAACCGACATACTCGCCCTTGCGGACAGAATCCG
GGGAGTGCCCGACACACACC

Downstream 100 bases:

>100_bases
AGGCTGTTCAATGTTCCCGGTTTCAATTCTGAACCCTGAACCAACATACCGAATTCCCACGAGGTTATACATATGACACT
TACCAGAATCCTGGTGGCCG

Product: signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNLPDSREENQIPLDDYYATVIDSVGDGVLVINREGLITLCNPAAEEITGLSRRHALGASFRKLFSHEATLLEMVNKTTR
TGVTISDHENVVVRGMARVTPVAVTCYPLMRANGENIGAILTLKDITYIRELEAAVRQADRLSTLGTLAAGLAHEVKNPL
GGIKGAAQLLEQELAAESELLEYTRVMIRETERIDRIIRELLELASPRGLKLAPVNLHRILGDILLLQKQAVADRQISFV
THFDPSIPDIMADREMLTRLFLNLIRNAIDAIAESGQLTVTSRVLSDYRLAQNQRRSRMVAVDIADDGPGIPGADLENIW
TPFFSSKSTGTGLGLTICHKIVSEHRGMIKVESDPGHGTKFTVLLPLVQ

Sequences:

>Translated_369_residues
MNLPDSREENQIPLDDYYATVIDSVGDGVLVINREGLITLCNPAAEEITGLSRRHALGASFRKLFSHEATLLEMVNKTTR
TGVTISDHENVVVRGMARVTPVAVTCYPLMRANGENIGAILTLKDITYIRELEAAVRQADRLSTLGTLAAGLAHEVKNPL
GGIKGAAQLLEQELAAESELLEYTRVMIRETERIDRIIRELLELASPRGLKLAPVNLHRILGDILLLQKQAVADRQISFV
THFDPSIPDIMADREMLTRLFLNLIRNAIDAIAESGQLTVTSRVLSDYRLAQNQRRSRMVAVDIADDGPGIPGADLENIW
TPFFSSKSTGTGLGLTICHKIVSEHRGMIKVESDPGHGTKFTVLLPLVQ
>Mature_369_residues
MNLPDSREENQIPLDDYYATVIDSVGDGVLVINREGLITLCNPAAEEITGLSRRHALGASFRKLFSHEATLLEMVNKTTR
TGVTISDHENVVVRGMARVTPVAVTCYPLMRANGENIGAILTLKDITYIRELEAAVRQADRLSTLGTLAAGLAHEVKNPL
GGIKGAAQLLEQELAAESELLEYTRVMIRETERIDRIIRELLELASPRGLKLAPVNLHRILGDILLLQKQAVADRQISFV
THFDPSIPDIMADREMLTRLFLNLIRNAIDAIAESGQLTVTSRVLSDYRLAQNQRRSRMVAVDIADDGPGIPGADLENIW
TPFFSSKSTGTGLGLTICHKIVSEHRGMIKVESDPGHGTKFTVLLPLVQ

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=351, Percent_Identity=34.4729344729345, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1790300, Length=350, Percent_Identity=30.2857142857143, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=37.037037037037, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI48994928, Length=377, Percent_Identity=26.525198938992, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI145693157, Length=276, Percent_Identity=27.8985507246377, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790567, Length=356, Percent_Identity=26.685393258427, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=356, Percent_Identity=21.6292134831461, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=27.2357723577236, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=239, Percent_Identity=25.1046025104602, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=257, Percent_Identity=25.2918287937743, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=28.6343612334802, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788393, Length=233, Percent_Identity=26.1802575107296, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788713, Length=386, Percent_Identity=22.020725388601, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=24.2857142857143, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40568; Mature: 40568

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPDSREENQIPLDDYYATVIDSVGDGVLVINREGLITLCNPAAEEITGLSRRHALGAS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHH
FRKLFSHEATLLEMVNKTTRTGVTISDHENVVVRGMARVTPVAVTCYPLMRANGENIGAI
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEHHHHHCHHHHEEHHHHHCCCCCCEEE
LTLKDITYIRELEAAVRQADRLSTLGTLAAGLAHEVKNPLGGIKGAAQLLEQELAAESEL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEYTRVMIRETERIDRIIRELLELASPRGLKLAPVNLHRILGDILLLQKQAVADRQISFV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHEEEE
THFDPSIPDIMADREMLTRLFLNLIRNAIDAIAESGQLTVTSRVLSDYRLAQNQRRSRMV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCEE
AVDIADDGPGIPGADLENIWTPFFSSKSTGTGLGLTICHKIVSEHRGMIKVESDPGHGTK
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCE
FTVLLPLVQ
EEEEEEECC
>Mature Secondary Structure
MNLPDSREENQIPLDDYYATVIDSVGDGVLVINREGLITLCNPAAEEITGLSRRHALGAS
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHH
FRKLFSHEATLLEMVNKTTRTGVTISDHENVVVRGMARVTPVAVTCYPLMRANGENIGAI
HHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEHHHHHCHHHHEEHHHHHCCCCCCEEE
LTLKDITYIRELEAAVRQADRLSTLGTLAAGLAHEVKNPLGGIKGAAQLLEQELAAESEL
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEYTRVMIRETERIDRIIRELLELASPRGLKLAPVNLHRILGDILLLQKQAVADRQISFV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHEEEE
THFDPSIPDIMADREMLTRLFLNLIRNAIDAIAESGQLTVTSRVLSDYRLAQNQRRSRMV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCEE
AVDIADDGPGIPGADLENIWTPFFSSKSTGTGLGLTICHKIVSEHRGMIKVESDPGHGTK
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCE
FTVLLPLVQ
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]