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The map label for this gene is dus1 [H]

Identifier: 118581480

GI number: 118581480

Start: 3365768

End: 3366757

Strand: Direct

Name: dus1 [H]

Synonym: Ppro_3077

Alternate gene names: 118581480

Gene position: 3365768-3366757 (Clockwise)

Preceding gene: 118581479

Following gene: 118581481

Centisome position: 83.98

GC content: 64.24

Gene sequence:

>990_bases
ATGCTGAAACCATTAAAAATTGGCTCGCTGACCCTGGCGCACAATGTCGTGCTCGCCCCCCTTGCCGGAATCACCAACCT
GCCCTTCCGCGTCATCTGCCGCCGCATGGGAGCGGCACTGGCATTCACCGAGATGGTCAGCGTCAATGGACTGGTGCGAT
CGGGCAGCAATACCCTGGCGCTGCTCAAGAGCACCCCCGAGGACCATCCCCTGGGTATCCAGCTCTTCGGCGATAGGCCG
GAGGATCTGGCCGAGGCGGCCCGCAGGGTTGAGGGGACAGGAGAGTTACTGGACATCAACATGGGCTGCCCGGTGCGCAA
GGTGGTGGGAACCGGCGCAGGCAGCGCCCTTCTGCGGGAACCGCTCAAAGTGGCCGCCATCATCCGGGCGGTGCGTGCGG
CAACCCCCCTGCCGCTGTCGGTCAAGATTCGTTCCGGCTGGCACTGCGGCGAGGACTCCTATCTCCGGATCGCCCAAATA
GCCCAATCCGAGGGATGCGACGCCATCACCCTGCACCCGCGCAGCCGCAGCCAGATGTTTTCCGGCCACGCCGACTGGGA
CCAGCTGCGGGAGTTGAAGTCCCAGCTCAGCATACCGGTCATCGGCAGCGGCGACCTGTTCACCCCCCAGGACTGCCAGC
GGATGTTGGCAGAGACCGGCTGCGACGGCGTCATGGTGGCCCGCGGAGCCCTGGGAACGCCCTGGATATTCAGACAGGTC
CTCGAACTGGGAGAGAGCGGCTCATTCCGCGAGATAACCAACGCCGAACGGGCCGACATGATCCGCCTGCACCTGGAACT
GTACCTGCAGACGTGGGGAGAATCCATCGCCAGCCGGGAGATGAAGAAACATATTGGCTGGTACGCCAAGGGGTTCGCCG
GCGCGGCGGACGTACGCCGGGAGGCCAACAACGCACGCTCGACAACCGACATACTCGCCCTTGCGGACAGAATCCGGGGA
GTGCCCGACACACACCATGAACCTACCTGA

Upstream 100 bases:

>100_bases
TTTCCCCTTGCCATTTAGCACAACCGTGCTATTATGCCCAAATTTTAGGCACAAATATGAACATCACGGAACGCCTCCTT
CCCACCAACATGCGCCTATC

Downstream 100 bases:

>100_bases
CTCACGAGAAGAAAACCAGATTCCGCTGGATGACTACTACGCCACGGTCATCGACAGCGTCGGCGACGGCGTGCTGGTCA
TCAACCGCGAGGGGCTGATC

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MLKPLKIGSLTLAHNVVLAPLAGITNLPFRVICRRMGAALAFTEMVSVNGLVRSGSNTLALLKSTPEDHPLGIQLFGDRP
EDLAEAARRVEGTGELLDINMGCPVRKVVGTGAGSALLREPLKVAAIIRAVRAATPLPLSVKIRSGWHCGEDSYLRIAQI
AQSEGCDAITLHPRSRSQMFSGHADWDQLRELKSQLSIPVIGSGDLFTPQDCQRMLAETGCDGVMVARGALGTPWIFRQV
LELGESGSFREITNAERADMIRLHLELYLQTWGESIASREMKKHIGWYAKGFAGAADVRREANNARSTTDILALADRIRG
VPDTHHEPT

Sequences:

>Translated_329_residues
MLKPLKIGSLTLAHNVVLAPLAGITNLPFRVICRRMGAALAFTEMVSVNGLVRSGSNTLALLKSTPEDHPLGIQLFGDRP
EDLAEAARRVEGTGELLDINMGCPVRKVVGTGAGSALLREPLKVAAIIRAVRAATPLPLSVKIRSGWHCGEDSYLRIAQI
AQSEGCDAITLHPRSRSQMFSGHADWDQLRELKSQLSIPVIGSGDLFTPQDCQRMLAETGCDGVMVARGALGTPWIFRQV
LELGESGSFREITNAERADMIRLHLELYLQTWGESIASREMKKHIGWYAKGFAGAADVRREANNARSTTDILALADRIRG
VPDTHHEPT
>Mature_329_residues
MLKPLKIGSLTLAHNVVLAPLAGITNLPFRVICRRMGAALAFTEMVSVNGLVRSGSNTLALLKSTPEDHPLGIQLFGDRP
EDLAEAARRVEGTGELLDINMGCPVRKVVGTGAGSALLREPLKVAAIIRAVRAATPLPLSVKIRSGWHCGEDSYLRIAQI
AQSEGCDAITLHPRSRSQMFSGHADWDQLRELKSQLSIPVIGSGDLFTPQDCQRMLAETGCDGVMVARGALGTPWIFRQV
LELGESGSFREITNAERADMIRLHLELYLQTWGESIASREMKKHIGWYAKGFAGAADVRREANNARSTTDILALADRIRG
VPDTHHEPT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=287, Percent_Identity=32.7526132404181, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI239788483, Length=286, Percent_Identity=32.5174825174825, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI40807366, Length=253, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=222, Percent_Identity=31.0810810810811, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI8923374, Length=250, Percent_Identity=27.2, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=310, Percent_Identity=35.4838709677419, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=35.1694915254237, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI145693211, Length=333, Percent_Identity=25.8258258258258, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=32.5174825174825, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=32.034632034632, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=214, Percent_Identity=31.3084112149533, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=292, Percent_Identity=30.4794520547945, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323560, Length=277, Percent_Identity=27.0758122743682, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=27.6785714285714, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24585320, Length=271, Percent_Identity=32.1033210332103, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=32.3144104803493, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=32.3144104803493, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=30.2222222222222, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=29.0322580645161, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35772; Mature: 35772

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPLKIGSLTLAHNVVLAPLAGITNLPFRVICRRMGAALAFTEMVSVNGLVRSGSNTLA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLKSTPEDHPLGIQLFGDRPEDLAEAARRVEGTGELLDINMGCPVRKVVGTGAGSALLRE
EEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHHH
PLKVAAIIRAVRAATPLPLSVKIRSGWHCGEDSYLRIAQIAQSEGCDAITLHPRSRSQMF
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHH
SGHADWDQLRELKSQLSIPVIGSGDLFTPQDCQRMLAETGCDGVMVARGALGTPWIFRQV
CCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LELGESGSFREITNAERADMIRLHLELYLQTWGESIASREMKKHIGWYAKGFAGAADVRR
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHH
EANNARSTTDILALADRIRGVPDTHHEPT
HHCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLKPLKIGSLTLAHNVVLAPLAGITNLPFRVICRRMGAALAFTEMVSVNGLVRSGSNTLA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LLKSTPEDHPLGIQLFGDRPEDLAEAARRVEGTGELLDINMGCPVRKVVGTGAGSALLRE
EEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCCHHHHHH
PLKVAAIIRAVRAATPLPLSVKIRSGWHCGEDSYLRIAQIAQSEGCDAITLHPRSRSQMF
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHH
SGHADWDQLRELKSQLSIPVIGSGDLFTPQDCQRMLAETGCDGVMVARGALGTPWIFRQV
CCCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHH
LELGESGSFREITNAERADMIRLHLELYLQTWGESIASREMKKHIGWYAKGFAGAADVRR
HHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHH
EANNARSTTDILALADRIRGVPDTHHEPT
HHCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]