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The map label for this gene is rpoD [H]

Identifier: 118581452

GI number: 118581452

Start: 3336693

End: 3338441

Strand: Reverse

Name: rpoD [H]

Synonym: Ppro_3049

Alternate gene names: 118581452

Gene position: 3338441-3336693 (Counterclockwise)

Preceding gene: 118581453

Following gene: 118581449

Centisome position: 83.29

GC content: 48.94

Gene sequence:

>1749_bases
ATGGTTAAAAAAAATATGGACGGCGTTAAGCAGTTGATTGATCTCGGCAAGGAAAAGGGTTTCCTGACCTTCGAAGAGGT
TAACGACATCCTGCCGCCAGATATTGCCACGGAACAGATAGATGACGTCATGGGCATGTTTGGCGATCTGGATATCGAGA
TCGTTGACTCTGCCCAGAAAGTCAAGATTCCGAAAATGAAGCTTGAGATGGAAGAAGAAGAGGAAGCGGAAGGTGAGGCC
GAAGAGGTCGAGTTTGAGCCTGGCTCCATCGGGCGTACAAGCGATCCGGTGCGCATGTATTTGCGTGAAATGGGCTCTGT
TTCCCTTTTGACCCGCGAAGGCGAAGTTGAGATTGCCAAGCGTATTGAGGATGGAGAGCGTGATGTTGCCAGCGTTATTC
TTAATACGCCGATTACCATAAAAGAGGTGCTGCAACTGGGAGATAAGCTTCGCAAATTCCAGCTGAATGCTTCTGAAATC
AGCAAAGAGGTTGAGGAAGAGTCTCTGGATGATGAGGAAGAAGATCTTCCCAAGCTGCGCATCCTTGAGATTATCGATGC
AATTTCAAGTGGTGATGAGGAATTGACTGGCCTTTCTGAAGAGCTTGAAAAATGTGCTGCCGCCGCCAAAAAGAAGGAAC
TTGAGAAAAAAATTCAGGAACTCAGGGAGTCGCAGGCAAAACTGCTGATCTCGCTGCGCCTGAAGGATCGCCACATAAAT
AAAATATCAGAACGCCTGAAGGAGTTGTCGTTTAAGGTCGACAGGCTCATGAAGGAATTGGCTGATCTGGAGGATCTGAT
ACCAGCTGAGAAACTCAGATTTTTCATGGACAGTTACCATAAGGATGAAGAAAAAGCTCAGGCCGAGTTGCGTAAGCTCA
AACTGGGCGCAGAAGAACAGGATAGAGTTGATGCCCGATTGCTTTCCACAGCGCGAAAGCTGAAGAAAATCGAACTGGAA
TCCGGTTTCAAGGCCAGCGAACTGTCAGCGGCGCTTCAGGCGATTGAAGAAGGTGAGTGTAAAGCGCGTATAGCGAAATC
CGAACTTATCGAAGCGAATCTTCGCCTGGTTGTATCCATTGCGAAAAAGTACACCAATCGAGGCTTGCAGTTTCTTGACC
TGATCCAAGAGGGCAACATCGGCCTTATGAAGGCGGTGGACAAGTTCGAATACCAGCGTGGTTACAAATTTTCTACTTAT
GCCACCTGGTGGATACGTCAGGCAATTACCCGTGCAATTGCCGATCAGGCACGAACCATACGGATTCCAGTGCACATGAT
CGAGACCATCAACAAGCTCATCCGCACCAGTCGCCAGTTGGTACAGGAAAATGGTCGTGAGCCGGCTCCTGAAGAAATTG
CGGAACGGATGCAATTGCCTCTGGATAAGGTTCGTAAAGTTTTGAAGATTGCCAAGGAGCCTATTTCGCTGGAGACTCCG
ATTGGCGAGGAAGAAGACTCGCATTTGGGCGATTTTATTGAGGATAAAGCGGTGATTTCTCCCATAGAAGCGGTCATTAA
GGCCAATCTGTCGGAGCAGACATCCAGGGTATTATCCACTCTTACTCCTCGCGAGGAGAAGGTCCTGCGTATGCGTTTCG
GTATCGGCGAAAAGAGTGACCACACTCTCGAGGAGGTTGGGCAGGACTTCGCCGTTACCCGTGAGCGGATTCGCCAGATA
GAGGCAAAGGCGCTGCGCAAACTGCGGCATCCCAGTCGCAGTAAAAAGCTCAAGAGCTTTGTTGAATAA

Upstream 100 bases:

>100_bases
GGTTGCGAGGAATATATGTCACTGCTGCAGCAGGCGGAACTGTTGCGCACCAGAAAATCAAGACTGTAGTCATATTGTTG
ACTGCGTTTGAGGTGAACGA

Downstream 100 bases:

>100_bases
CCCTGGTACTGATTTTAGTGCCGTTGATCAAAAAAGCCCCGTACGGTATTGACCGTTCAGGGCTTCTTTTGTTTGTGTGT
CTACTGCGCACCTCATTGAT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MVKKNMDGVKQLIDLGKEKGFLTFEEVNDILPPDIATEQIDDVMGMFGDLDIEIVDSAQKVKIPKMKLEMEEEEEAEGEA
EEVEFEPGSIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGERDVASVILNTPITIKEVLQLGDKLRKFQLNASEI
SKEVEEESLDDEEEDLPKLRILEIIDAISSGDEELTGLSEELEKCAAAAKKKELEKKIQELRESQAKLLISLRLKDRHIN
KISERLKELSFKVDRLMKELADLEDLIPAEKLRFFMDSYHKDEEKAQAELRKLKLGAEEQDRVDARLLSTARKLKKIELE
SGFKASELSAALQAIEEGECKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTY
ATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPAPEEIAERMQLPLDKVRKVLKIAKEPISLETP
IGEEEDSHLGDFIEDKAVISPIEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFAVTRERIRQI
EAKALRKLRHPSRSKKLKSFVE

Sequences:

>Translated_582_residues
MVKKNMDGVKQLIDLGKEKGFLTFEEVNDILPPDIATEQIDDVMGMFGDLDIEIVDSAQKVKIPKMKLEMEEEEEAEGEA
EEVEFEPGSIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGERDVASVILNTPITIKEVLQLGDKLRKFQLNASEI
SKEVEEESLDDEEEDLPKLRILEIIDAISSGDEELTGLSEELEKCAAAAKKKELEKKIQELRESQAKLLISLRLKDRHIN
KISERLKELSFKVDRLMKELADLEDLIPAEKLRFFMDSYHKDEEKAQAELRKLKLGAEEQDRVDARLLSTARKLKKIELE
SGFKASELSAALQAIEEGECKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTY
ATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPAPEEIAERMQLPLDKVRKVLKIAKEPISLETP
IGEEEDSHLGDFIEDKAVISPIEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFAVTRERIRQI
EAKALRKLRHPSRSKKLKSFVE
>Mature_582_residues
MVKKNMDGVKQLIDLGKEKGFLTFEEVNDILPPDIATEQIDDVMGMFGDLDIEIVDSAQKVKIPKMKLEMEEEEEAEGEA
EEVEFEPGSIGRTSDPVRMYLREMGSVSLLTREGEVEIAKRIEDGERDVASVILNTPITIKEVLQLGDKLRKFQLNASEI
SKEVEEESLDDEEEDLPKLRILEIIDAISSGDEELTGLSEELEKCAAAAKKKELEKKIQELRESQAKLLISLRLKDRHIN
KISERLKELSFKVDRLMKELADLEDLIPAEKLRFFMDSYHKDEEKAQAELRKLKLGAEEQDRVDARLLSTARKLKKIELE
SGFKASELSAALQAIEEGECKARIAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTY
ATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRQLVQENGREPAPEEIAERMQLPLDKVRKVLKIAKEPISLETP
IGEEEDSHLGDFIEDKAVISPIEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSDHTLEEVGQDFAVTRERIRQI
EAKALRKLRHPSRSKKLKSFVE

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=71.5867158671587, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=42.7350427350427, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=27.8571428571429, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 66413; Mature: 66413

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKNMDGVKQLIDLGKEKGFLTFEEVNDILPPDIATEQIDDVMGMFGDLDIEIVDSAQK
CCCCCHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHH
VKIPKMKLEMEEEEEAEGEAEEVEFEPGSIGRTSDPVRMYLREMGSVSLLTREGEVEIAK
CCCCHHHCCCCHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RIEDGERDVASVILNTPITIKEVLQLGDKLRKFQLNASEISKEVEEESLDDEEEDLPKLR
HHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCHHHH
ILEIIDAISSGDEELTGLSEELEKCAAAAKKKELEKKIQELRESQAKLLISLRLKDRHIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISERLKELSFKVDRLMKELADLEDLIPAEKLRFFMDSYHKDEEKAQAELRKLKLGAEEQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
DRVDARLLSTARKLKKIELESGFKASELSAALQAIEEGECKARIAKSELIEANLRLVVSI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIADQARTI
HHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RIPVHMIETINKLIRTSRQLVQENGREPAPEEIAERMQLPLDKVRKVLKIAKEPISLETP
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
IGEEEDSHLGDFIEDKAVISPIEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
HTLEEVGQDFAVTRERIRQIEAKALRKLRHPSRSKKLKSFVE
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MVKKNMDGVKQLIDLGKEKGFLTFEEVNDILPPDIATEQIDDVMGMFGDLDIEIVDSAQK
CCCCCHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCHHH
VKIPKMKLEMEEEEEAEGEAEEVEFEPGSIGRTSDPVRMYLREMGSVSLLTREGEVEIAK
CCCCHHHCCCCHHHHHCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHH
RIEDGERDVASVILNTPITIKEVLQLGDKLRKFQLNASEISKEVEEESLDDEEEDLPKLR
HHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCHHHH
ILEIIDAISSGDEELTGLSEELEKCAAAAKKKELEKKIQELRESQAKLLISLRLKDRHIN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISERLKELSFKVDRLMKELADLEDLIPAEKLRFFMDSYHKDEEKAQAELRKLKLGAEEQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
DRVDARLLSTARKLKKIELESGFKASELSAALQAIEEGECKARIAKSELIEANLRLVVSI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRGYKFSTYATWWIRQAITRAIADQARTI
HHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEE
RIPVHMIETINKLIRTSRQLVQENGREPAPEEIAERMQLPLDKVRKVLKIAKEPISLETP
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
IGEEEDSHLGDFIEDKAVISPIEAVIKANLSEQTSRVLSTLTPREEKVLRMRFGIGEKSD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
HTLEEVGQDFAVTRERIRQIEAKALRKLRHPSRSKKLKSFVE
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]