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The map label for this gene is dnaG [H]

Identifier: 118581453

GI number: 118581453

Start: 3338473

End: 3340227

Strand: Reverse

Name: dnaG [H]

Synonym: Ppro_3050

Alternate gene names: 118581453

Gene position: 3340227-3338473 (Counterclockwise)

Preceding gene: 118581454

Following gene: 118581452

Centisome position: 83.34

GC content: 52.93

Gene sequence:

>1755_bases
ATGATTCCAGATCATAAAGTTCGCGAGGTAGTGGAGCGCGCTTCGATTGTCGAGGTCGTTTCCGAGTATGTCCCGTTGCG
ACGCTCTGGCGCGAATTATCTTGGCCTCTGTCCGTTTCACGCCGAAAAGACACCATCTTTTAATGTTAACCCGGCACGTG
AGATTTTCCACTGCTTTGGTTGCGGCGTTGGCGGTAACGTCATTTCGTTTGTAATGAAGATCGAAGGCTTAAGCTTTCCC
GAATCGGTGAAGCTGCTTGCTCGCAAAACAGGCGTGGATATCGAAGAGCGCCTGCTTACGCCGGCGGAAAAGCAGGCCCA
GGAAGAACACAAGATTTTTCGGCGGATACAGGAGTTGGCAGCCGCATACTATTGTGGTGTGTTGCAACGCGCCTCGGAGG
CTGGACTAGCCAGGGAGTATCTGGAAAAGCGGAATGCCAGTGGTCAGATAGCTGAAACCTATGGACTTGGATTTGCGCCT
GACCGACGTGATGGACTTGTGCAGTACCTCAAATCCCAGGGTGCAAGCCTTGACCTTGCCCTGAAACTGGGAATTGTCCG
TAAGGGTGATCGTGGCTGGCATGACCTTTTCCGCAACCGGTTAATCTTTCCCGTCCGGTCACCCAAGGGTGATGTGATTG
CCTTTGCGGGGCGCGTGCTGGACGGATCGTTGCCCAAATATATTAATTCGCCTGAGTCGCCGCTCTACCATAAAAGCTCG
GTTCTCTTTGGTCTCGACTTGGCGCTGCCGGCGATCAGGACCGAAAACAGTGTCATCATCGTCGAGGGGTACTTTGACCA
CCTTGCTCTGTATCGGGCAGGCGTCCGTAACGTGGTAGCTACATGCGGTACTGCGCTGACCGCACGTCATGCCGCCTTGA
TCAAGAGGCATTGCGCCAGGGCGTTCACGCTTTTTGATGGTGATTCTGCCGGTCAAAAAGCAACCATACGGTCAATGGAG
CTTTTCCTGGAGCAGAAGCTGCCGGCGTATGTCATTGACCTGCCTGCCGGAGATGATCCTGATAGCTTTCTTGATGCAAA
TTCTGTGGAGGCATTTCGCTCCTGTCAGAAAAACGCGAAGCCGGCGTTCGAGTATTTCATTCGCTATGTAATGGCTTCGA
TCCCGGCGGACAGTGTTGACAGCAAGGTCAGGATTGTCGATGAGTTGGTACCCCGTTTTCGTAAGATAGCCGATCCGCTG
GAGCGCGACCTGTATGAAAAGGAAATCTGCCGTCTGCTGGGGATTGCGTCGCATGCGTTTCGAAAACGTCTGGGGGGGAT
GGATGTCAATAGCCATGATCTTACGGAAGGCGGGAAGGGCGGCACGCCACCGGGAGACTCGCGTCAGGAAACACTTCTGG
GTCTCATCTGCGCCTACCCGGAGGCATGCAGCGAGGTTGCCAAGTGTGGTGTTGACAAGTTGTTTGATGGTCAGTATCTG
GCTCTGGCGCGGTTCGTGTTGGATGAGGTGGCGGCAAACGGACAATTACGAACCTTGAGCCATGTAGTTGATAAAATTGA
ATCGGAAGAATTGAAGCTCTTTCTTTCTCGTCTGCTGGTATCAGATAACCACCTGGAAGGTGTCGATTGGAAAGATGTCT
TTGAAAGTTGCCGACTGATCAGGGAAAAAAGTGCGCTTCACTATGGTTATAAGGATATTGCCGCCCGACTGGCGCAGCTT
GAGCCAGGTTGCGAGGAATATATGTCACTGCTGCAGCAGGCGGAACTGTTGCGCACCAGAAAATCAAGACTGTAG

Upstream 100 bases:

>100_bases
AACTGAAACAGAAAATACAAATGACCGAATCAGCACATATGTTCGCACAAATTGGTGATAAACTTCTCTTTACGTGGCGC
GGGAGGCCTGGAGTCCAACG

Downstream 100 bases:

>100_bases
TCATATTGTTGACTGCGTTTGAGGTGAACGAATGGTTAAAAAAAATATGGACGGCGTTAAGCAGTTGATTGATCTCGGCA
AGGAAAAGGGTTTCCTGACC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MIPDHKVREVVERASIVEVVSEYVPLRRSGANYLGLCPFHAEKTPSFNVNPAREIFHCFGCGVGGNVISFVMKIEGLSFP
ESVKLLARKTGVDIEERLLTPAEKQAQEEHKIFRRIQELAAAYYCGVLQRASEAGLAREYLEKRNASGQIAETYGLGFAP
DRRDGLVQYLKSQGASLDLALKLGIVRKGDRGWHDLFRNRLIFPVRSPKGDVIAFAGRVLDGSLPKYINSPESPLYHKSS
VLFGLDLALPAIRTENSVIIVEGYFDHLALYRAGVRNVVATCGTALTARHAALIKRHCARAFTLFDGDSAGQKATIRSME
LFLEQKLPAYVIDLPAGDDPDSFLDANSVEAFRSCQKNAKPAFEYFIRYVMASIPADSVDSKVRIVDELVPRFRKIADPL
ERDLYEKEICRLLGIASHAFRKRLGGMDVNSHDLTEGGKGGTPPGDSRQETLLGLICAYPEACSEVAKCGVDKLFDGQYL
ALARFVLDEVAANGQLRTLSHVVDKIESEELKLFLSRLLVSDNHLEGVDWKDVFESCRLIREKSALHYGYKDIAARLAQL
EPGCEEYMSLLQQAELLRTRKSRL

Sequences:

>Translated_584_residues
MIPDHKVREVVERASIVEVVSEYVPLRRSGANYLGLCPFHAEKTPSFNVNPAREIFHCFGCGVGGNVISFVMKIEGLSFP
ESVKLLARKTGVDIEERLLTPAEKQAQEEHKIFRRIQELAAAYYCGVLQRASEAGLAREYLEKRNASGQIAETYGLGFAP
DRRDGLVQYLKSQGASLDLALKLGIVRKGDRGWHDLFRNRLIFPVRSPKGDVIAFAGRVLDGSLPKYINSPESPLYHKSS
VLFGLDLALPAIRTENSVIIVEGYFDHLALYRAGVRNVVATCGTALTARHAALIKRHCARAFTLFDGDSAGQKATIRSME
LFLEQKLPAYVIDLPAGDDPDSFLDANSVEAFRSCQKNAKPAFEYFIRYVMASIPADSVDSKVRIVDELVPRFRKIADPL
ERDLYEKEICRLLGIASHAFRKRLGGMDVNSHDLTEGGKGGTPPGDSRQETLLGLICAYPEACSEVAKCGVDKLFDGQYL
ALARFVLDEVAANGQLRTLSHVVDKIESEELKLFLSRLLVSDNHLEGVDWKDVFESCRLIREKSALHYGYKDIAARLAQL
EPGCEEYMSLLQQAELLRTRKSRL
>Mature_584_residues
MIPDHKVREVVERASIVEVVSEYVPLRRSGANYLGLCPFHAEKTPSFNVNPAREIFHCFGCGVGGNVISFVMKIEGLSFP
ESVKLLARKTGVDIEERLLTPAEKQAQEEHKIFRRIQELAAAYYCGVLQRASEAGLAREYLEKRNASGQIAETYGLGFAP
DRRDGLVQYLKSQGASLDLALKLGIVRKGDRGWHDLFRNRLIFPVRSPKGDVIAFAGRVLDGSLPKYINSPESPLYHKSS
VLFGLDLALPAIRTENSVIIVEGYFDHLALYRAGVRNVVATCGTALTARHAALIKRHCARAFTLFDGDSAGQKATIRSME
LFLEQKLPAYVIDLPAGDDPDSFLDANSVEAFRSCQKNAKPAFEYFIRYVMASIPADSVDSKVRIVDELVPRFRKIADPL
ERDLYEKEICRLLGIASHAFRKRLGGMDVNSHDLTEGGKGGTPPGDSRQETLLGLICAYPEACSEVAKCGVDKLFDGQYL
ALARFVLDEVAANGQLRTLSHVVDKIESEELKLFLSRLLVSDNHLEGVDWKDVFESCRLIREKSALHYGYKDIAARLAQL
EPGCEEYMSLLQQAELLRTRKSRL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=433, Percent_Identity=32.3325635103926, Blast_Score=241, Evalue=7e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 64997; Mature: 64997

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDHKVREVVERASIVEVVSEYVPLRRSGANYLGLCPFHAEKTPSFNVNPAREIFHCFG
CCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHC
CGVGGNVISFVMKIEGLSFPESVKLLARKTGVDIEERLLTPAEKQAQEEHKIFRRIQELA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAYYCGVLQRASEAGLAREYLEKRNASGQIAETYGLGFAPDRRDGLVQYLKSQGASLDLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEE
LKLGIVRKGDRGWHDLFRNRLIFPVRSPKGDVIAFAGRVLDGSLPKYINSPESPLYHKSS
EEECCEECCCCCHHHHHHCCEEEEECCCCCCEEEEHHHHHCCCCHHHCCCCCCCCEECCC
VLFGLDLALPAIRTENSVIIVEGYFDHLALYRAGVRNVVATCGTALTARHAALIKRHCAR
HHEEHHHHHHCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTLFDGDSAGQKATIRSMELFLEQKLPAYVIDLPAGDDPDSFLDANSVEAFRSCQKNAK
HHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHCCC
PAFEYFIRYVMASIPADSVDSKVRIVDELVPRFRKIADPLERDLYEKEICRLLGIASHAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLGGMDVNSHDLTEGGKGGTPPGDSRQETLLGLICAYPEACSEVAKCGVDKLFDGQYL
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHCCCHHH
ALARFVLDEVAANGQLRTLSHVVDKIESEELKLFLSRLLVSDNHLEGVDWKDVFESCRLI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
REKSALHYGYKDIAARLAQLEPGCEEYMSLLQQAELLRTRKSRL
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPDHKVREVVERASIVEVVSEYVPLRRSGANYLGLCPFHAEKTPSFNVNPAREIFHCFG
CCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHC
CGVGGNVISFVMKIEGLSFPESVKLLARKTGVDIEERLLTPAEKQAQEEHKIFRRIQELA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
AAYYCGVLQRASEAGLAREYLEKRNASGQIAETYGLGFAPDRRDGLVQYLKSQGASLDLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCEEEE
LKLGIVRKGDRGWHDLFRNRLIFPVRSPKGDVIAFAGRVLDGSLPKYINSPESPLYHKSS
EEECCEECCCCCHHHHHHCCEEEEECCCCCCEEEEHHHHHCCCCHHHCCCCCCCCEECCC
VLFGLDLALPAIRTENSVIIVEGYFDHLALYRAGVRNVVATCGTALTARHAALIKRHCAR
HHEEHHHHHHCEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFTLFDGDSAGQKATIRSMELFLEQKLPAYVIDLPAGDDPDSFLDANSVEAFRSCQKNAK
HHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHCCC
PAFEYFIRYVMASIPADSVDSKVRIVDELVPRFRKIADPLERDLYEKEICRLLGIASHAF
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRLGGMDVNSHDLTEGGKGGTPPGDSRQETLLGLICAYPEACSEVAKCGVDKLFDGQYL
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHCCCHHH
ALARFVLDEVAANGQLRTLSHVVDKIESEELKLFLSRLLVSDNHLEGVDWKDVFESCRLI
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
REKSALHYGYKDIAARLAQLEPGCEEYMSLLQQAELLRTRKSRL
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]