Click here to switch to the map view.

The map label for this gene is pilJ [H]

Identifier: 118579594

GI number: 118579594

Start: 1213549

End: 1215303

Strand: Reverse

Name: pilJ [H]

Synonym: Ppro_1163

Alternate gene names: 118579594

Gene position: 1215303-1213549 (Counterclockwise)

Preceding gene: 118579595

Following gene: 118579593

Centisome position: 30.32

GC content: 65.01

Gene sequence:

>1755_bases
GTGAAGTGGATCTCGTCTTCTGACAGCGTACGCGCATACTCCCTGCGAACAGAGATGGCTCTGCTCTTCCTGACGGCAGC
AACTGCCATCCTGATCCTGGCCGCCATCTGTCTGATGTCGCTCCCCCAGGGGGATGGTGCGGTTCTGTCCTCCCTGGTCG
CCGCGGGGATCAGGGAGGGGGAATCATCCAGCCTGCTGGTGATCCTGACTGTAGCGATTGGCACCGGCCTGGTGGCCTGG
CTGTCGTTCGGCATAACCCGCTCCCATACCCGCTCGCTGGCTTCCTGTACCCGCCTGGCGCGGGCATTGTGCGACGGCAG
GTTCGATGAGCCTCCGGCGACAGCCGCCAACACGGAAACGGCCTTTCTCGCCGCTTCCCTGAACACCATGGCCGCGCGCC
TCCAGCACCAACGGTCCGCAATCGATGCCATCTGCGGGGCGTTGACCTCCCTTGACGATGTACTGAAGACTGACAGCGCC
CGCATGGAGCACTCCTTTCGACGTCAGGAATCGGACATGCGCCACCTGGCTCCCGAAATAGCGCGCATGGAGCAGAGCCT
GAAGGACATTTCCCGCGGCACGGAGAGCCTGCTGCCGACAGCATCGTCAAGCAGCAACAGCACCCGCGCCATGGTTAGCG
CCAGCGAACGGATTTCCGCCATCAACGACAACCTGGGGGCCTCCCTGGACGAGATCGGGGCTGCCACGGACAGAATGGAT
GCTGCCGCCAGAACCTCCGCACTCGCCATCTCGGATATGCTGGCCCTTTACGGAACAGGCGCCTCGTCCTGCAACCGCAT
GGAGGCCGCCATCAGGCAGGCCGAGAAGGACGGCCAGGAGACCCGGGCCATAGCGGAGGGGATTACCCGTGACGCTGATG
CCGGCAGACGCGTGGCCCAGGAAGCCATCAACGCCATGCGAGCCATCAGGGGTGCCTCGGCAACCACGACGGAGGCGGTA
ACGAGCCTGTCCCGACGGGCAGAGGACACGGACGCCATCCTCTCGGTAATCGAGGAACTGGCCGAACAGACCGACCTTCT
GGCACTGAACGCCACCATCATCGCCGCCCAGGCCGGCGACATGGGGAGAGATTTCAGCGTCGTTGCCGACGAGATCAGGG
ACCTGTCGGAACGGACCGGCAGTTCAACCCGCGAAATAGCCGCCATCATCCACGGCATACGGGACGAAGGGCGTCGCGCG
GTGGACGCCATCAGCCAGGTGGAAGAGCTCATCGCAACGGGCGAGCAGCTCGCCAGACACACCAGCATGGCGCTGGAAAT
GATCGTCAACGGCGTGCAGCAGGCAGTCCTGCAGATGGGCGGCATCACCCGCAACGACAGGGAGCAGGCCCGAACCAGCC
AGGACATCATGGATGCCCTGCGGCGCACCGGCGAACTGGCGCAACGAATTGCCCGTACCTCCAGCGACCAGCGCCAACTA
ACGGAACTATCCATAGCCACGCTGGAGCGCATGGGAGGACTGACCAGCCAGATACACTCCCTGGCCCGCGAGCAGCGCCA
CGGCGGCGCCCTGGCGCTCCAGGCTGCGGGCAGCCTGACCAACGGCATACGGCAACTGCGCGATACCGCTGCAGCCGCCC
CCTTGGGCAGCGCACCGCCCACCGCCACCCTGGCAGGGCTCCAGACGGCAGCCAGCGCGTCGGGCGACGACATCAGGGCA
CTGGATGGCTCCCTGACCGCCCTGGGGGAACAGATCAGGCTGCTGAGAAGCGAACTGTCGGACTTCAGTGCATAA

Upstream 100 bases:

>100_bases
AGACGGGGCTTTTTGCTTGATAACGGAGCGCCCGCTCTGTAAGATACACTCCCTGCTTCAGGATGTGCCAATCCCTCATT
CCACAAAGGAATCTCGCCCG

Downstream 100 bases:

>100_bases
CCCCACTCCACACGCAACGGACACCACAATGAACAGACTCACCCACTGCTTCAACGAACTCAAGCAGCGCAACACAAAGG
CGCTGGTCACCTTCATCACC

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MKWISSSDSVRAYSLRTEMALLFLTAATAILILAAICLMSLPQGDGAVLSSLVAAGIREGESSSLLVILTVAIGTGLVAW
LSFGITRSHTRSLASCTRLARALCDGRFDEPPATAANTETAFLAASLNTMAARLQHQRSAIDAICGALTSLDDVLKTDSA
RMEHSFRRQESDMRHLAPEIARMEQSLKDISRGTESLLPTASSSSNSTRAMVSASERISAINDNLGASLDEIGAATDRMD
AAARTSALAISDMLALYGTGASSCNRMEAAIRQAEKDGQETRAIAEGITRDADAGRRVAQEAINAMRAIRGASATTTEAV
TSLSRRAEDTDAILSVIEELAEQTDLLALNATIIAAQAGDMGRDFSVVADEIRDLSERTGSSTREIAAIIHGIRDEGRRA
VDAISQVEELIATGEQLARHTSMALEMIVNGVQQAVLQMGGITRNDREQARTSQDIMDALRRTGELAQRIARTSSDQRQL
TELSIATLERMGGLTSQIHSLAREQRHGGALALQAAGSLTNGIRQLRDTAAAAPLGSAPPTATLAGLQTAASASGDDIRA
LDGSLTALGEQIRLLRSELSDFSA

Sequences:

>Translated_584_residues
MKWISSSDSVRAYSLRTEMALLFLTAATAILILAAICLMSLPQGDGAVLSSLVAAGIREGESSSLLVILTVAIGTGLVAW
LSFGITRSHTRSLASCTRLARALCDGRFDEPPATAANTETAFLAASLNTMAARLQHQRSAIDAICGALTSLDDVLKTDSA
RMEHSFRRQESDMRHLAPEIARMEQSLKDISRGTESLLPTASSSSNSTRAMVSASERISAINDNLGASLDEIGAATDRMD
AAARTSALAISDMLALYGTGASSCNRMEAAIRQAEKDGQETRAIAEGITRDADAGRRVAQEAINAMRAIRGASATTTEAV
TSLSRRAEDTDAILSVIEELAEQTDLLALNATIIAAQAGDMGRDFSVVADEIRDLSERTGSSTREIAAIIHGIRDEGRRA
VDAISQVEELIATGEQLARHTSMALEMIVNGVQQAVLQMGGITRNDREQARTSQDIMDALRRTGELAQRIARTSSDQRQL
TELSIATLERMGGLTSQIHSLAREQRHGGALALQAAGSLTNGIRQLRDTAAAAPLGSAPPTATLAGLQTAASASGDDIRA
LDGSLTALGEQIRLLRSELSDFSA
>Mature_584_residues
MKWISSSDSVRAYSLRTEMALLFLTAATAILILAAICLMSLPQGDGAVLSSLVAAGIREGESSSLLVILTVAIGTGLVAW
LSFGITRSHTRSLASCTRLARALCDGRFDEPPATAANTETAFLAASLNTMAARLQHQRSAIDAICGALTSLDDVLKTDSA
RMEHSFRRQESDMRHLAPEIARMEQSLKDISRGTESLLPTASSSSNSTRAMVSASERISAINDNLGASLDEIGAATDRMD
AAARTSALAISDMLALYGTGASSCNRMEAAIRQAEKDGQETRAIAEGITRDADAGRRVAQEAINAMRAIRGASATTTEAV
TSLSRRAEDTDAILSVIEELAEQTDLLALNATIIAAQAGDMGRDFSVVADEIRDLSERTGSSTREIAAIIHGIRDEGRRA
VDAISQVEELIATGEQLARHTSMALEMIVNGVQQAVLQMGGITRNDREQARTSQDIMDALRRTGELAQRIARTSSDQRQL
TELSIATLERMGGLTSQIHSLAREQRHGGALALQAAGSLTNGIRQLRDTAAAAPLGSAPPTATLAGLQTAASASGDDIRA
LDGSLTALGEQIRLLRSELSDFSA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788195, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI2367378, Length=191, Percent_Identity=28.7958115183246, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788194, Length=185, Percent_Identity=27.5675675675676, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 61701; Mature: 61701

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWISSSDSVRAYSLRTEMALLFLTAATAILILAAICLMSLPQGDGAVLSSLVAAGIREG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
ESSSLLVILTVAIGTGLVAWLSFGITRSHTRSLASCTRLARALCDGRFDEPPATAANTET
CCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AFLAASLNTMAARLQHQRSAIDAICGALTSLDDVLKTDSARMEHSFRRQESDMRHLAPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARMEQSLKDISRGTESLLPTASSSSNSTRAMVSASERISAINDNLGASLDEIGAATDRMD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHH
AAARTSALAISDMLALYGTGASSCNRMEAAIRQAEKDGQETRAIAEGITRDADAGRRVAQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
EAINAMRAIRGASATTTEAVTSLSRRAEDTDAILSVIEELAEQTDLLALNATIIAAQAGD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEHHHEEEECCCC
MGRDFSVVADEIRDLSERTGSSTREIAAIIHGIRDEGRRAVDAISQVEELIATGEQLARH
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSMALEMIVNGVQQAVLQMGGITRNDREQARTSQDIMDALRRTGELAQRIARTSSDQRQL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TELSIATLERMGGLTSQIHSLAREQRHGGALALQAAGSLTNGIRQLRDTAAAAPLGSAPP
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
TATLAGLQTAASASGDDIRALDGSLTALGEQIRLLRSELSDFSA
CHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKWISSSDSVRAYSLRTEMALLFLTAATAILILAAICLMSLPQGDGAVLSSLVAAGIREG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
ESSSLLVILTVAIGTGLVAWLSFGITRSHTRSLASCTRLARALCDGRFDEPPATAANTET
CCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
AFLAASLNTMAARLQHQRSAIDAICGALTSLDDVLKTDSARMEHSFRRQESDMRHLAPEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARMEQSLKDISRGTESLLPTASSSSNSTRAMVSASERISAINDNLGASLDEIGAATDRMD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHH
AAARTSALAISDMLALYGTGASSCNRMEAAIRQAEKDGQETRAIAEGITRDADAGRRVAQ
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
EAINAMRAIRGASATTTEAVTSLSRRAEDTDAILSVIEELAEQTDLLALNATIIAAQAGD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEHHHEEEECCCC
MGRDFSVVADEIRDLSERTGSSTREIAAIIHGIRDEGRRAVDAISQVEELIATGEQLARH
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSMALEMIVNGVQQAVLQMGGITRNDREQARTSQDIMDALRRTGELAQRIARTSSDQRQL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TELSIATLERMGGLTSQIHSLAREQRHGGALALQAAGSLTNGIRQLRDTAAAAPLGSAPP
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCC
TATLAGLQTAASASGDDIRALDGSLTALGEQIRLLRSELSDFSA
CHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]