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The map label for this gene is cobD

Identifier: 118579307

GI number: 118579307

Start: 913240

End: 914211

Strand: Reverse

Name: cobD

Synonym: Ppro_0870

Alternate gene names: 118579307

Gene position: 914211-913240 (Counterclockwise)

Preceding gene: 118579308

Following gene: 118579306

Centisome position: 22.81

GC content: 66.87

Gene sequence:

>972_bases
GTGATCCAGCCTGACCCCACCGTCCTGGCCCTGGCGCTGCTGCTGGACCTGTGCCTGGGTGATCCGCGCTGGCTGCCCCA
CCCGGTGGTGATGATCGGACGCCTGATCACCTTCCTGGAGACACTGCTGCGCAGATGCATGGCCAACGAGCGCATTGCCG
GTGTGCTGCTGCTGGCCCTGACCGTTACCTCTGCCGCCTCGGTCACCTGGCTGATGGTGTGGGGCTCGGCCCGACTGCAC
GCCTTGGCGGGGCTCATGGTTGCCGCCCTGCTCAGCTCCACCTGCCTGGCGGCGCGTTCCCTGCAGCGCGAGTCATGCCT
GGTGGCCGATGCCCTCGACGCCGGCGACATTGCCAGCGCACGCGTCAAACTCTCCTACATCGTCGGCCGCGACACCGTTG
ATCTGGATGAAGAGGAGATCTGGCGCGCCCTGATCGAAACCGTGGCCGAAAACACCACCGACGGCATCATCGCTCCCCTG
TTCTGGCTGGCCCTGGGGGGACCGGTGGCCGGCATGGCCTTCAAGGCGGTCAGCACCCTGGACTCCATGGTGGGCTACAA
AAACGAGCGCTACCTGCGCCTCGGGTGGGCCTCGGCCCGCATGGACGACCTGGTGAACTACATCCCGGCCCGCCTGACCG
CCCTGCTGATGGTCATGGTCGCGCCGCTGATCGGCCTCTCCCAGGCTAACGCGGCCAGCATAGCCCTGCGGGACCGGCTG
AACCACCCCTCACCCAACAGCGCCCACCCCGAGTCGGCTGCGGCCGGCGCCCTGGGAATACGCTTGGGAGGGCCATCCAC
CTACGGCGGCCTGCTGTCGGTCAAGCAGTTCATCGGCGACCCGCTGCGCAGCATAGACGGCCAGGCCTATCGCGGCATGA
TCAGACTGATGTACGCCACCACCCTTGCCATGGCCGTCATCTCCCTGGCAACGGCCGCACTCCTGAGAGGTATCCATGTC
ACACAGCTATGA

Upstream 100 bases:

>100_bases
GAGACTCGCCAGCCTGTCAGCGACCCCTTCGACCTGCTCTCCTGCCACATGGAGCGGCATCTGGACATGCCGAAGCTGCT
GGAAATCTGCGGACTGCCCC

Downstream 100 bases:

>100_bases
GCACGGCGGCAACCTGTTTTCCCTGGCCCGCCAGCGCGGCTTTTCCCCCGCCGATTCCATCGACTTCTCGGCCAGCATCA
ACCCGCTGGGCATCTCCCCC

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLALTVTSAASVTWLMVWGSARLH
ALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASARVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPL
FWLALGGPVAGMAFKAVSTLDSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRL
NHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYATTLAMAVISLATAALLRGIHV
TQL

Sequences:

>Translated_323_residues
MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLALTVTSAASVTWLMVWGSARLH
ALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASARVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPL
FWLALGGPVAGMAFKAVSTLDSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRL
NHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYATTLAMAVISLATAALLRGIHV
TQL
>Mature_323_residues
MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLALTVTSAASVTWLMVWGSARLH
ALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASARVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPL
FWLALGGPVAGMAFKAVSTLDSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRL
NHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYATTLAMAVISLATAALLRGIHV
TQL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_PELPD (A1AMC9)

Other databases:

- EMBL:   CP000482
- RefSeq:   YP_900557.1
- STRING:   A1AMC9
- GeneID:   4572106
- GenomeReviews:   CP000482_GR
- KEGG:   ppd:Ppro_0870
- eggNOG:   COG1270
- HOGENOM:   HBG647131
- OMA:   RWHPLVG
- ProtClustDB:   CLSK829041
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34479; Mature: 34479

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2305fc28)-; HASH(0x2214abe0)-; HASH(0x2371f1d4)-; HASH(0x234219c8)-; HASH(0x2230f8f4)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLAL
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TVTSAASVTWLMVWGSARLHALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASA
HHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPLFWLALGGPVAGMAFKAVSTL
HEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRL
HHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYAT
CCCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHH
TLAMAVISLATAALLRGIHVTQL
HHHHHHHHHHHHHHHHCCHHCCH
>Mature Secondary Structure
MIQPDPTVLALALLLDLCLGDPRWLPHPVVMIGRLITFLETLLRRCMANERIAGVLLLAL
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TVTSAASVTWLMVWGSARLHALAGLMVAALLSSTCLAARSLQRESCLVADALDAGDIASA
HHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
RVKLSYIVGRDTVDLDEEEIWRALIETVAENTTDGIIAPLFWLALGGPVAGMAFKAVSTL
HEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
DSMVGYKNERYLRLGWASARMDDLVNYIPARLTALLMVMVAPLIGLSQANAASIALRDRL
HHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NHPSPNSAHPESAAAGALGIRLGGPSTYGGLLSVKQFIGDPLRSIDGQAYRGMIRLMYAT
CCCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHH
TLAMAVISLATAALLRGIHVTQL
HHHHHHHHHHHHHHHHCCHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA