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The map label for this gene is lptD [H]

Identifier: 117921644

GI number: 117921644

Start: 3848821

End: 3851118

Strand: Direct

Name: lptD [H]

Synonym: Shewana3_3206

Alternate gene names: 117921644

Gene position: 3848821-3851118 (Clockwise)

Preceding gene: 117921638

Following gene: 117921645

Centisome position: 77.41

GC content: 48.91

Gene sequence:

>2298_bases
ATGCAGATCCGTTACTTTCTGGCCTTGAGCCTGTTACCGCAAGTTGTCCTGGCAGACGAATCTCCCACAGCCTCAGCGTC
ACAATGCGTCATTGAACCGCCAGTACCTCGTATCGTATCTCAACCCGGCCTGAGCGCCGCCGATCAAGAGAAAATTCGTA
TCGTGTCGGACCGCTCCAACGCCGAAATGGGCAAACAAGCCATTTTTACCGGTGATGTGGTCTTCAGTCAGGGCGACAGA
CACATTGCCGCCGATGAAGCGATTCTCGACCAAGCGACAGAGCAGTTCGATGCCAATGGCAACCTGGTTTTCCAAGACAG
TATTTTTACCGTTACCGCCGATTCGCTGCAGGCACAAATGCGCAGCAACCGTGCGACCCTAAAGGGTGCCCAGTATTGGC
TACATGGTCAGCAGGTTCACGGCGATGCTGAAAAGCTGCAAATCACCATGAACAACAACCTGATCTTAACCAACACTAAC
TTCACCACTTGTCCGCCAGACAATGTTTCTTGGCTACTCGAAGCAGAAAAAATCAAGATTAACAGCGAAGAAGAATGGGG
CGAGATTTGGAATGCTAAGCTGAGAGTCGCCGATATTCCGGTGTTCTACATTCCTTATATGACGGTGCCAGTTTCCGACA
AACGTAAAACCGGTTTCCTCTACCCAAGCTTTAGCACCAGCACCACCAATGGTTTTGAAGTTTCCGCGCCCTATTATTGG
AATATCGCGCCGGAATACGACCTCACCTTTACACCTAACTATATGTCTAGCCGTGGTTTATTCACCAAGACAGAGTTTCG
TTATCTCGCGGGTGAGGCCCAGAGTGGTCGCTTGAATTTAGAATATTTGGGTAATGATCAGATGATCAGCGGCAGCCCAA
ATCGTTACCTCTATAACTGGCAACACCAAGGCGCCATCGATAAAAACTGGCGGGTACTCGCCAACTTTACTGAAGTGTCT
GATAACAACTATTTCAACGACTTAAAGTCTGATGTTAACCGCGCCACCGATAACCAATTATCGCGGATTGGTGAAGTCAG
CTACTTCGAACGAGATTGGGATATCAGCACTCGGGTGCAAGATATTAAAGTGCTGGGTGAGGACGAAAAGCCTTATCAAG
TGATGCCGCAGGTGAATTTTAACTACCGCGCCGCCGACTTTTGGAACAACTTGGACTTTGGCTTTAATTCAGAGTTGACC
AACTTCGCCCATCAAGACAGCGACATGAATACCGCCACCCGTTTGCATATGGCACCTAGCCTGACGCTGCCAATTCACGG
CCCTTCGGGTTCGTTGACCAGCCAAGTCAAACTGATGCAAACCAACTATTGGCAGGAGAAAAACAACAGTGGATTCGATG
GCTTAGACGATACTGTGAGTCGCACCATTCCGCAGGTGCGGATCAATGGTCAAATCAACTTCGAGCGTTTTACCGAACTG
TTCGATCAAAACTACCGCCAGACGCTCGAACCTCAGTTCCAGTATCTGTACGTAGGTTATGAAGATCAGCGTGGCATCGG
CATTTACGATACCGCCCAGTTACAGGACGACTACTTCGGTCTGTTCCGTGACCGCCGCTTCTCAGGCCTTGACCGCATCG
CCGATGCAAACCAAGTCACGCTCGGTGTAACAACGCGTTTCTTCGACGATCACAACCAAGAGGCGACTAAGTTCAGCCTA
GGTCAGATCCTCTATCTGCAGGACAGTAAACTGGGTTACGAAGATAACCTCTTCGAGCAAAATCAATCGACCTCAGTCTT
GGCCGCCGAGTTAGATACCCGCTTAAGCCATGACTGGTATTTAGGCGCAGCGATTCAATACGACACCAACTCGAGCGATA
ACAAGAAGACCGAAGTCACCTTAGACTTCCGTCCCGAAGCCAATAAACTATTGCAGCTCAGTTATCGTTATGTGCCGGAT
CTATTGAACTCCAACACCAACGATCTGGTGAATATTTCCCAAGCGGGTGTGCGTGGTGCCTGGCCGATTAACGACAGCCT
GTATTTTGTCGGTAACTGGTACTACGACCTCAACGAGAGCCGCAGCATAGAAACCTATACTGGTTTCCAATATGAATCAT
GCTGTTACGCAATTCGTCTCAGCTATCACTACCGAATTAAGACCAATTACGATGACAACATTGGTTCGGCCGTTATCGAT
GAGCGCGAGCAATTTGAAAGCGGTGTTTACCTGAATTTAGTCATCAAAGGACTCGGTGGTTCGGGCCCATTAGGCGTATC
CGATATGCTCAATGACGGTCTGTTTAACTACAGAAAACCGCTTTATCTGAGGAATTAG

Upstream 100 bases:

>100_bases
CTAAGAAATTGAGAGATTTATTTGCTGCGTTGCACTTGTAGTTTGCAACTTTCCCCGTCGATACTTGTCCAGACAAAGAA
ATCATTATTATTGACCTAAG

Downstream 100 bases:

>100_bases
CAAAGATTTGTGGTAGATTGCTGAACCTCAACAAGGACTGAGAGTCCAAACAGGTTAATGAAAGCCGTGCGCGCCCCAAA
AAAGAGTCTCGGAACGCACG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MQIRYFLALSLLPQVVLADESPTASASQCVIEPPVPRIVSQPGLSAADQEKIRIVSDRSNAEMGKQAIFTGDVVFSQGDR
HIAADEAILDQATEQFDANGNLVFQDSIFTVTADSLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITMNNNLILTNTN
FTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVADIPVFYIPYMTVPVSDKRKTGFLYPSFSTSTTNGFEVSAPYYW
NIAPEYDLTFTPNYMSSRGLFTKTEFRYLAGEAQSGRLNLEYLGNDQMISGSPNRYLYNWQHQGAIDKNWRVLANFTEVS
DNNYFNDLKSDVNRATDNQLSRIGEVSYFERDWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELT
NFAHQDSDMNTATRLHMAPSLTLPIHGPSGSLTSQVKLMQTNYWQEKNNSGFDGLDDTVSRTIPQVRINGQINFERFTEL
FDQNYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRIADANQVTLGVTTRFFDDHNQEATKFSL
GQILYLQDSKLGYEDNLFEQNQSTSVLAAELDTRLSHDWYLGAAIQYDTNSSDNKKTEVTLDFRPEANKLLQLSYRYVPD
LLNSNTNDLVNISQAGVRGAWPINDSLYFVGNWYYDLNESRSIETYTGFQYESCCYAIRLSYHYRIKTNYDDNIGSAVID
EREQFESGVYLNLVIKGLGGSGPLGVSDMLNDGLFNYRKPLYLRN

Sequences:

>Translated_765_residues
MQIRYFLALSLLPQVVLADESPTASASQCVIEPPVPRIVSQPGLSAADQEKIRIVSDRSNAEMGKQAIFTGDVVFSQGDR
HIAADEAILDQATEQFDANGNLVFQDSIFTVTADSLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITMNNNLILTNTN
FTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVADIPVFYIPYMTVPVSDKRKTGFLYPSFSTSTTNGFEVSAPYYW
NIAPEYDLTFTPNYMSSRGLFTKTEFRYLAGEAQSGRLNLEYLGNDQMISGSPNRYLYNWQHQGAIDKNWRVLANFTEVS
DNNYFNDLKSDVNRATDNQLSRIGEVSYFERDWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELT
NFAHQDSDMNTATRLHMAPSLTLPIHGPSGSLTSQVKLMQTNYWQEKNNSGFDGLDDTVSRTIPQVRINGQINFERFTEL
FDQNYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRIADANQVTLGVTTRFFDDHNQEATKFSL
GQILYLQDSKLGYEDNLFEQNQSTSVLAAELDTRLSHDWYLGAAIQYDTNSSDNKKTEVTLDFRPEANKLLQLSYRYVPD
LLNSNTNDLVNISQAGVRGAWPINDSLYFVGNWYYDLNESRSIETYTGFQYESCCYAIRLSYHYRIKTNYDDNIGSAVID
EREQFESGVYLNLVIKGLGGSGPLGVSDMLNDGLFNYRKPLYLRN
>Mature_765_residues
MQIRYFLALSLLPQVVLADESPTASASQCVIEPPVPRIVSQPGLSAADQEKIRIVSDRSNAEMGKQAIFTGDVVFSQGDR
HIAADEAILDQATEQFDANGNLVFQDSIFTVTADSLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITMNNNLILTNTN
FTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVADIPVFYIPYMTVPVSDKRKTGFLYPSFSTSTTNGFEVSAPYYW
NIAPEYDLTFTPNYMSSRGLFTKTEFRYLAGEAQSGRLNLEYLGNDQMISGSPNRYLYNWQHQGAIDKNWRVLANFTEVS
DNNYFNDLKSDVNRATDNQLSRIGEVSYFERDWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELT
NFAHQDSDMNTATRLHMAPSLTLPIHGPSGSLTSQVKLMQTNYWQEKNNSGFDGLDDTVSRTIPQVRINGQINFERFTEL
FDQNYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRIADANQVTLGVTTRFFDDHNQEATKFSL
GQILYLQDSKLGYEDNLFEQNQSTSVLAAELDTRLSHDWYLGAAIQYDTNSSDNKKTEVTLDFRPEANKLLQLSYRYVPD
LLNSNTNDLVNISQAGVRGAWPINDSLYFVGNWYYDLNESRSIETYTGFQYESCCYAIRLSYHYRIKTNYDDNIGSAVID
EREQFESGVYLNLVIKGLGGSGPLGVSDMLNDGLFNYRKPLYLRN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=789, Percent_Identity=34.4740177439797, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 87407; Mature: 87407

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRYFLALSLLPQVVLADESPTASASQCVIEPPVPRIVSQPGLSAADQEKIRIVSDRSN
CEEEEEEHHHHHCCEEEECCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCEEEEECCCC
AEMGKQAIFTGDVVFSQGDRHIAADEAILDQATEQFDANGNLVFQDSIFTVTADSLQAQM
CCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHH
RSNRATLKGAQYWLHGQQVHGDAEKLQITMNNNLILTNTNFTTCPPDNVSWLLEAEKIKI
HCCCCEEECCEEEEECCEECCCCEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEEE
NSEEEWGEIWNAKLRVADIPVFYIPYMTVPVSDKRKTGFLYPSFSTSTTNGFEVSAPYYW
CCCHHHHHHCCCEEEEEECCEEEECEEEEECCCCCCCEEEECCCCCCCCCCEEEECCEEE
NIAPEYDLTFTPNYMSSRGLFTKTEFRYLAGEAQSGRLNLEYLGNDQMISGSPNRYLYNW
ECCCCCCEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCEECCCCCCEEEEE
QHQGAIDKNWRVLANFTEVSDNNYFNDLKSDVNRATDNQLSRIGEVSYFERDWDISTRVQ
ECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEE
DIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELTNFAHQDSDMNTATRLHMAPS
EEEEECCCCCCEEEECCCCCCEEEHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCC
LTLPIHGPSGSLTSQVKLMQTNYWQEKNNSGFDGLDDTVSRTIPQVRINGQINFERFTEL
EEEEEECCCCCCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHCCCEEEECCEECHHHHHHH
FDQNYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRIADANQVT
HHHHHHHHCCCCEEEEEEEECCCCCCCEEECHHCCCHHHHHHHHCCCCCHHHHCCCCEEE
LGVTTRFFDDHNQEATKFSLGQILYLQDSKLGYEDNLFEQNQSTSVLAAELDTRLSHDWY
EEEEEEEECCCCCCCCEEECCEEEEEECCCCCCCCCHHCCCCCCEEEEEEHHHHCCCCEE
LGAAIQYDTNSSDNKKTEVTLDFRPEANKLLQLSYRYVPDLLNSNTNDLVNISQAGVRGA
EEEEEEECCCCCCCCEEEEEEEECCCCCHHEEEHHHHHHHHHCCCCCCEEEECCCCCCCC
WPINDSLYFVGNWYYDLNESRSIETYTGFQYESCCYAIRLSYHYRIKTNYDDNIGSAVID
CCCCCCEEEEEEEEEECCCCCCEEEECCCCHHCEEEEEEEEEEEEEECCCCCCCCHHHHC
EREQFESGVYLNLVIKGLGGSGPLGVSDMLNDGLFNYRKPLYLRN
CHHHHHCCEEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC
>Mature Secondary Structure
MQIRYFLALSLLPQVVLADESPTASASQCVIEPPVPRIVSQPGLSAADQEKIRIVSDRSN
CEEEEEEHHHHHCCEEEECCCCCCCCCCEEECCCCHHHHCCCCCCCCCCCCEEEEECCCC
AEMGKQAIFTGDVVFSQGDRHIAADEAILDQATEQFDANGNLVFQDSIFTVTADSLQAQM
CCCCCEEEEECCEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCEEEEEHHHHHHHH
RSNRATLKGAQYWLHGQQVHGDAEKLQITMNNNLILTNTNFTTCPPDNVSWLLEAEKIKI
HCCCCEEECCEEEEECCEECCCCEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEEE
NSEEEWGEIWNAKLRVADIPVFYIPYMTVPVSDKRKTGFLYPSFSTSTTNGFEVSAPYYW
CCCHHHHHHCCCEEEEEECCEEEECEEEEECCCCCCCEEEECCCCCCCCCCEEEECCEEE
NIAPEYDLTFTPNYMSSRGLFTKTEFRYLAGEAQSGRLNLEYLGNDQMISGSPNRYLYNW
ECCCCCCEEECCCCCCCCCCEEECCEEEEECCCCCCEEEEEECCCCCEECCCCCCEEEEE
QHQGAIDKNWRVLANFTEVSDNNYFNDLKSDVNRATDNQLSRIGEVSYFERDWDISTRVQ
ECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEE
DIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELTNFAHQDSDMNTATRLHMAPS
EEEEECCCCCCEEEECCCCCCEEEHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCC
LTLPIHGPSGSLTSQVKLMQTNYWQEKNNSGFDGLDDTVSRTIPQVRINGQINFERFTEL
EEEEEECCCCCCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHCCCEEEECCEECHHHHHHH
FDQNYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRIADANQVT
HHHHHHHHCCCCEEEEEEEECCCCCCCEEECHHCCCHHHHHHHHCCCCCHHHHCCCCEEE
LGVTTRFFDDHNQEATKFSLGQILYLQDSKLGYEDNLFEQNQSTSVLAAELDTRLSHDWY
EEEEEEEECCCCCCCCEEECCEEEEEECCCCCCCCCHHCCCCCCEEEEEEHHHHCCCCEE
LGAAIQYDTNSSDNKKTEVTLDFRPEANKLLQLSYRYVPDLLNSNTNDLVNISQAGVRGA
EEEEEEECCCCCCCCEEEEEEEECCCCCHHEEEHHHHHHHHHCCCCCCEEEECCCCCCCC
WPINDSLYFVGNWYYDLNESRSIETYTGFQYESCCYAIRLSYHYRIKTNYDDNIGSAVID
CCCCCCEEEEEEEEEECCCCCCEEEECCCCHHCEEEEEEEEEEEEEECCCCCCCCHHHHC
EREQFESGVYLNLVIKGLGGSGPLGVSDMLNDGLFNYRKPLYLRN
CHHHHHCCEEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]