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The map label for this gene is purR [H]

Identifier: 117921560

GI number: 117921560

Start: 3748018

End: 3749034

Strand: Direct

Name: purR [H]

Synonym: Shewana3_3122

Alternate gene names: 117921560

Gene position: 3748018-3749034 (Clockwise)

Preceding gene: 117921556

Following gene: 117921563

Centisome position: 75.38

GC content: 43.26

Gene sequence:

>1017_bases
ATGAAAGTCACTATCAACGACGTCGCTAAATATGCTGGAGTGTCAATCAAGACAGTTTCCCGCGTAACCAATAACGAACC
TTCAGTAAAACAAGCCACGGTTGATAAAGTGAATGAGGCCATCAAGGCATTAAACTACCAGCCCAATCTCGCGGCCAGAA
ATCTTGCTGGCACAAAATCCTATGCCATTGGTTTTATTTATGATAATCCCAACGCTTATTACATTATTGATATGCAAAAC
GGGATTTTGTCTGCCTGTAAAGATAAAGGCTATGAACTGGTTATTCATCCTTGTAACGCCAAATCTGACACCATTTGCGA
TGAACTGACTCAACTAGTGAAACATTCGCGACTGGCAGGTTTAGTGTTAACACCGCCTCTTTCTGAAGATCCTAAAGTCC
TTAAAGCCTTAAGTGATATTGAGGCCAACTATGTGCGAATTATTGCCGGCGAAGGTAATAAAGAGCAGGATGGACTGACG
ATTTTAGTCAATGACAAATTTGGTGCGGTAGAAATTACTCAGCATTTGATCGACTTAGGCCATAAAGAAATTGCCTTTTT
AAGTGGCGATCTACACCACGAGTCCACCAAAGAGCGGTTACTTGGTTATAAACAAGCCTTAACAAAAAATCGCCTGCTTC
TGAATGAAGATTACATAATTGAAGGAAAATACTCATTTGAGTCTGGCGTTGAAGGGGCTAACACACTGTTCAAACAGAAG
AACCGTCCAACAGCGATTGTTGCCTGTAACGACGAAATTGCAGCAGGGGCACTTTTTGCAGCTCGTTTAGCAGGGCTAGA
TATCCCTGGCGATCTATCCATTGTCGGCTTCGAAGACAGCCCATTCTCACGCCAAACTTGGCCAAAACTAACAACAGTGC
ATCAACCTAACGCTGAAATAGCCCAAGTGGCAACAGAGCTGTTAATCGCGAAACGCCGTGAACAAGATGCCAAGTTTGCA
AAAACATTTACGCCAGAGCCTGTGATCCGAGACTCTTCAGCCTCTCCCAAACATTAA

Upstream 100 bases:

>100_bases
GTAAGCTGACAACGCTGTCGGCTTGTAAATTGCGCCTAAAGGATTTAGGTTCATCAATTAGCTAGCCCTTCGCACATTTG
AATAAAGAGTTTGTAGATCA

Downstream 100 bases:

>100_bases
TTTTTAGCTCAGCAAGGCTAAAACAACACAGAGAGCCAAGGCTCTCTGTTTTGTTTTCAGTTAATGCAATTTCAGCTTTG
GCCTTTGCCATTTGAGTAAC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILSACKDKGYELVIHPCNAKSDTICDELTQLVKHSRLAGLVLTPPLSEDPKVLKALSDIEANYVRIIAGEGNKEQDGLT
ILVNDKFGAVEITQHLIDLGHKEIAFLSGDLHHESTKERLLGYKQALTKNRLLLNEDYIIEGKYSFESGVEGANTLFKQK
NRPTAIVACNDEIAAGALFAARLAGLDIPGDLSIVGFEDSPFSRQTWPKLTTVHQPNAEIAQVATELLIAKRREQDAKFA
KTFTPEPVIRDSSASPKH

Sequences:

>Translated_338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILSACKDKGYELVIHPCNAKSDTICDELTQLVKHSRLAGLVLTPPLSEDPKVLKALSDIEANYVRIIAGEGNKEQDGLT
ILVNDKFGAVEITQHLIDLGHKEIAFLSGDLHHESTKERLLGYKQALTKNRLLLNEDYIIEGKYSFESGVEGANTLFKQK
NRPTAIVACNDEIAAGALFAARLAGLDIPGDLSIVGFEDSPFSRQTWPKLTTVHQPNAEIAQVATELLIAKRREQDAKFA
KTFTPEPVIRDSSASPKH
>Mature_338_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKSYAIGFIYDNPNAYYIIDMQN
GILSACKDKGYELVIHPCNAKSDTICDELTQLVKHSRLAGLVLTPPLSEDPKVLKALSDIEANYVRIIAGEGNKEQDGLT
ILVNDKFGAVEITQHLIDLGHKEIAFLSGDLHHESTKERLLGYKQALTKNRLLLNEDYIIEGKYSFESGVEGANTLFKQK
NRPTAIVACNDEIAAGALFAARLAGLDIPGDLSIVGFEDSPFSRQTWPKLTTVHQPNAEIAQVATELLIAKRREQDAKFA
KTFTPEPVIRDSSASPKH

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=30.2250803858521, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787948, Length=317, Percent_Identity=30.9148264984227, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789202, Length=341, Percent_Identity=31.3782991202346, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789068, Length=344, Percent_Identity=31.3953488372093, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=29.3768545994065, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=31.1764705882353, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=29.0419161676647, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI48994940, Length=312, Percent_Identity=27.5641025641026, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787906, Length=264, Percent_Identity=28.7878787878788, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789456, Length=331, Percent_Identity=27.7945619335347, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=23.4375, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=23.8095238095238, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37061; Mature: 37061

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
YAIGFIYDNPNAYYIIDMQNGILSACKDKGYELVIHPCNAKSDTICDELTQLVKHSRLAG
EEEEEEEECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKVLKALSDIEANYVRIIAGEGNKEQDGLTILVNDKFGAVEITQHLIDLG
EEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKEIAFLSGDLHHESTKERLLGYKQALTKNRLLLNEDYIIEGKYSFESGVEGANTLFKQK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCHHCCCCHHHHHHHCC
NRPTAIVACNDEIAAGALFAARLAGLDIPGDLSIVGFEDSPFSRQTWPKLTTVHQPNAEI
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHH
AQVATELLIAKRREQDAKFAKTFTPEPVIRDSSASPKH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATVDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCC
YAIGFIYDNPNAYYIIDMQNGILSACKDKGYELVIHPCNAKSDTICDELTQLVKHSRLAG
EEEEEEEECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKVLKALSDIEANYVRIIAGEGNKEQDGLTILVNDKFGAVEITQHLIDLG
EEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKEIAFLSGDLHHESTKERLLGYKQALTKNRLLLNEDYIIEGKYSFESGVEGANTLFKQK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCHHCCCCHHHHHHHCC
NRPTAIVACNDEIAAGALFAARLAGLDIPGDLSIVGFEDSPFSRQTWPKLTTVHQPNAEI
CCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHH
AQVATELLIAKRREQDAKFAKTFTPEPVIRDSSASPKH
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA