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The map label for this gene is ptsP [H]

Identifier: 117921481

GI number: 117921481

Start: 3645655

End: 3647889

Strand: Reverse

Name: ptsP [H]

Synonym: Shewana3_3042

Alternate gene names: 117921481

Gene position: 3647889-3645655 (Counterclockwise)

Preceding gene: 117921482

Following gene: 117921480

Centisome position: 73.37

GC content: 51.59

Gene sequence:

>2235_bases
GTGTTAAATACGCTCAGGGATATTACTCAAGCTGTGGCGTCCGCCCACAGCTTAGAAACCGCATTAGAAGTCCTAGTTTC
ATCGACCAAAGCGGCGATGGAAACCCAATGTTGCTCGGTCTACATCCTAGATCAGCAAGAACTCGTATTATCAGCCACCG
ATGGCCTCGAAAAGAGTGCCGTTGGGCGCGTGCGCATGCCGTTAACCCAAGGCTTGGTTGGCTTGGCCGCCGAACGTGAA
GAAGCCGTAAACCTTGCTGATGCGCGTTTGCACCCACGCTTTAAGCTCTTCCCCGAAGTTGCTGAAGAAGAATACCGTGC
CTTTTTAGCCGTCCCCATTATTTATCAAAAAGCCGTCGTCGGCGTAATTGTGGTGCAGCAGGCCAGCGCGCGGCAATTTA
GCGAAGGGGAAGAAGCCTTCTTAATGACCTTGGCCGCGCAGCTCGCGATGGCGATCCGGGGATTAAAGCAAAAGGCGCAG
GTCAGTTCGCTGCATCAACAGATTTTATTTCAAGGAACCTCTGCATCCAGCGGTATCGCCATTGCCCATGCCTTGGTGCT
CGGTGGCGAAATCTCCCTCGAGCAGCCCGATGTTCGCTGTGAGGATATTGTCCTCGAATCCAGCCGATTAGTGGCGGCGA
TGGGGCGTTGTAAAGAGGCTATTGGTGCCTTATCTCAACGTTTCGACCGTGAGCAGGATGAGGAAGTCGCCTCAATATTC
AATGCGTTGCAGCTGCTGCTCGATGATGTCAGCCTAGGTGGTGAATATGCTCGCGAAGTGCAGCAGGGCTGGGAAGCAGA
ATCGGCGGTTAGCCGTGTTTCGCTGCGTTATATTCAACAGTTTTTAGCGATGGAAGATCCTTACCTCAAGGAGCGGGCGA
GCGATATTCGGGATCTGGGGCAAAAGGTATTAAGGCAGTTAATCGAACCCGAGCGTTTAGAGCTTGAACCCGATAAGCCG
GTGATTCTAGTGACCCGTGAAGCGGATGCCACTATGCTCGCCGAATTCCCTCGGCAAAAATTGGCCGGTATTGTCACTGA
ACTGGGAGGTGTTAACTCCCACGCGGCGATTTTAGCGCGCGCACTGGGCGTACCTGCGATTACTGGCGTCGAGCAGTTAT
TGTCGGCCGATATTGATCAGAAGCTGTTAGTGGTCAATGCCAGCCGCGGGCAATTAATGGTGTCGCCATCGCCAGCGATT
GTTAGCGAATACCGCAGTTTGATTTCGGCGCAAAAGGCGTTGCAACGTCAATACGCGCAGGAGTTAGCCCTGCCGTCGGT
CATGCTCGATGGCACTCGCATTCGGTTATACCTTAACGCTGGGTTACTCAGTGGTGTCGCTTCTGAAATTGCCGAAGGTG
CCGATGGTATCGGGCTGTATCGCACCGAAATTCCCTTTATGTTGCAGCAGCGTTTTCCGAGTGAGTCCGAGCAGGTAAAA
GTCTATCAGCAGGTGTTATCGGCGGCATCGGGTCGCCCTGTGGTGATGCGTACCTTAGATGTGGGAGGCGATAAGCCGCT
GCCTTATTTCCCTATTAAAGAAGATAACCCCTTCCTAGGTTGGCGCGGTATCCGCTTATCATTGGATCACCCAGAGCTAT
TCCTCGTTCAGTTAAGAGCGATGTTGCAAGCGGGCGGAGAGTGCAAACAGCTCAGTATTTTACTGCCTATGGTCAGTAAT
TTAGATGAGATTGATCAATCCTTGGCCTATCTAGAGCAGGCCTATGTTGAACTCAAAAACGATGTGAACAGCCAGATTGA
AATGCCGCGCATTGGCATTATGCTCGAAGTTCCAGCGCTGTTATATCAGTTAGATGAAGTGGCAAAACGCGTCGATTTTG
TGTCGGTCGGCAGTAACGATTTGACCCAATATTTACTCGCGGTGGACCGTAACAACCCAAGGGTGAGTTCCTTATTTGAT
AGTTATCATCCAGGCATTCTTCGGGCACTGCATCAGGCAAGACTCGATTGTGACTATCATGAACTCGATATCAGTATCTG
TGGCGAGCTGGCGGGGGAACCCATGGGGGCGATTCTGTTGGTGGCGATGGGATACCAGCATTTGAGTATGAACCAAGGCA
GTTTAGCGCGGATAAATTACCTGCTGCGCCGAGTGTCCCGCGCCGATTTGACGCAACTCTTGTCGCAGGCCTTAAGTCTG
TCGAACGGTTTCCAAGTGCGTGAGTTGGTTAAAGAATATTTAACACAGCAGGGATTAGCCACAATTCTTAATTAA

Upstream 100 bases:

>100_bases
AGTTTGCGGTAACGGCATTGTCATTCCAAACCCAGGAAATTCCGAGGAAGCGGGTAAGACAAAGAACAACGGGCTGATTT
TCAAAAGAGGATTGAAAACA

Downstream 100 bases:

>100_bases
CTAATCGCGCCTTTTGCTTTAAGCTAAGGCTTCACTTAATCACGCTGTAGGTACCCGTGTGGATAGTTTGCTGTCGGTGT
TCTTTATTTGCTTGGCTTTA

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSVYILDQQELVLSATDGLEKSAVGRVRMPLTQGLVGLAAERE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAIRGLKQKAQ
VSSLHQQILFQGTSASSGIAIAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRFDREQDEEVASIF
NALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILVTREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSADIDQKLLVVNASRGQLMVSPSPAI
VSEYRSLISAQKALQRQYAQELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGECKQLSILLPMVSN
LDEIDQSLAYLEQAYVELKNDVNSQIEMPRIGIMLEVPALLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQARLDCDYHELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRRVSRADLTQLLSQALSL
SNGFQVRELVKEYLTQQGLATILN

Sequences:

>Translated_744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSVYILDQQELVLSATDGLEKSAVGRVRMPLTQGLVGLAAERE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAIRGLKQKAQ
VSSLHQQILFQGTSASSGIAIAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRFDREQDEEVASIF
NALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILVTREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSADIDQKLLVVNASRGQLMVSPSPAI
VSEYRSLISAQKALQRQYAQELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGECKQLSILLPMVSN
LDEIDQSLAYLEQAYVELKNDVNSQIEMPRIGIMLEVPALLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQARLDCDYHELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRRVSRADLTQLLSQALSL
SNGFQVRELVKEYLTQQGLATILN
>Mature_744_residues
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSVYILDQQELVLSATDGLEKSAVGRVRMPLTQGLVGLAAERE
EAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVVGVIVVQQASARQFSEGEEAFLMTLAAQLAMAIRGLKQKAQ
VSSLHQQILFQGTSASSGIAIAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRFDREQDEEVASIF
NALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLGQKVLRQLIEPERLELEPDKP
VILVTREADATMLAEFPRQKLAGIVTELGGVNSHAAILARALGVPAITGVEQLLSADIDQKLLVVNASRGQLMVSPSPAI
VSEYRSLISAQKALQRQYAQELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRAMLQAGGECKQLSILLPMVSN
LDEIDQSLAYLEQAYVELKNDVNSQIEMPRIGIMLEVPALLYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFD
SYHPGILRALHQARLDCDYHELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRRVSRADLTQLLSQALSL
SNGFQVRELVKEYLTQQGLATILN

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=746, Percent_Identity=44.2359249329759, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1788756, Length=597, Percent_Identity=32.6633165829146, Blast_Score=299, Evalue=5e-82,
Organism=Escherichia coli, GI48994992, Length=512, Percent_Identity=32.8125, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1788726, Length=597, Percent_Identity=33.500837520938, Blast_Score=272, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 81815; Mature: 81815

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSVYILDQQELVLSATDGLEKSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCCHHH
VGRVRMPLTQGLVGLAAEREEAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVV
CCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQASARQFSEGEEAFLMTLAAQLAMAIRGLKQKAQVSSLHQQILFQGTSASSGIA
HHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
IAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRFDREQDEEVASIF
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLG
HHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QKVLRQLIEPERLELEPDKPVILVTREADATMLAEFPRQKLAGIVTELGGVNSHAAILAR
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALGVPAITGVEQLLSADIDQKLLVVNASRGQLMVSPSPAIVSEYRSLISAQKALQRQYAQ
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
HHCCCCEEECCCEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCHHHHH
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAGGECKQLSILLPMVSNLDEIDQSLAYLEQAYVELKNDVNSQIEMPRIGIMLEVPAL
HHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHH
LYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFDSYHPGILRALHQARLDCDYH
HHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHH
ELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRRVSRADLTQLLSQALSL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNGFQVRELVKEYLTQQGLATILN
CCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNTLRDITQAVASAHSLETALEVLVSSTKAAMETQCCSVYILDQQELVLSATDGLEKSA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHEEECCCCCCHHH
VGRVRMPLTQGLVGLAAEREEAVNLADARLHPRFKLFPEVAEEEYRAFLAVPIIYQKAVV
CCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHH
GVIVVQQASARQFSEGEEAFLMTLAAQLAMAIRGLKQKAQVSSLHQQILFQGTSASSGIA
HHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
IAHALVLGGEISLEQPDVRCEDIVLESSRLVAAMGRCKEAIGALSQRFDREQDEEVASIF
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
NALQLLLDDVSLGGEYAREVQQGWEAESAVSRVSLRYIQQFLAMEDPYLKERASDIRDLG
HHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QKVLRQLIEPERLELEPDKPVILVTREADATMLAEFPRQKLAGIVTELGGVNSHAAILAR
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ALGVPAITGVEQLLSADIDQKLLVVNASRGQLMVSPSPAIVSEYRSLISAQKALQRQYAQ
HHCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ELALPSVMLDGTRIRLYLNAGLLSGVASEIAEGADGIGLYRTEIPFMLQQRFPSESEQVK
HHCCCCEEECCCEEEEEEECHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCCHHHHH
VYQQVLSAASGRPVVMRTLDVGGDKPLPYFPIKEDNPFLGWRGIRLSLDHPELFLVQLRA
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MLQAGGECKQLSILLPMVSNLDEIDQSLAYLEQAYVELKNDVNSQIEMPRIGIMLEVPAL
HHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHH
LYQLDEVAKRVDFVSVGSNDLTQYLLAVDRNNPRVSSLFDSYHPGILRALHQARLDCDYH
HHHHHHHHHHHCEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCHH
ELDISICGELAGEPMGAILLVAMGYQHLSMNQGSLARINYLLRRVSRADLTQLLSQALSL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNGFQVRELVKEYLTQQGLATILN
CCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]