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The map label for this gene is smpB [H]

Identifier: 117921392

GI number: 117921392

Start: 3545220

End: 3545711

Strand: Direct

Name: smpB [H]

Synonym: Shewana3_2952

Alternate gene names: 117921392

Gene position: 3545220-3545711 (Clockwise)

Preceding gene: 117921389

Following gene: 117921393

Centisome position: 71.3

GC content: 46.54

Gene sequence:

>492_bases
ATGGTAAAGAAAAACTCAAAAAAAGCCGCGCCTGCGACAATCGCACGCAATAAGCGCGCAACCTTCGAATACCGTTTCGA
AGAAAAAATGGAAGCCGGCATTTCCCTCATGGGATGGGAGGTGAAGTCTATCCGCATGGGTAAAGTGAACCTATCGGACT
GCTACGTCTTCTTAAAAAATGGTGAAGCCTTTATGCACGGCTGTACCATTATTCCGCTTAATACCGCATCGACCCATGTG
GTGTGCGACCCGATTCGACTGAAGAAATTGCTCTTAAGTCGTAAAGAGTTAGATAAATTAGCTGGTCTTGTTGAGCGCCA
AGGCTACTCGATTATCCCTATCTCCATGTACTGGCGTAAAGGGGCTTGGGTCAAAGTAGAAATCGGCCTAGGTAAAGGTA
AAAAAGACCACGACAAGCGTGAAGATACCAAAGCACGCGAGTGGGAAGTGGAAAAAGCCCGCGTAATGAAAAAGGAAAAA
ACGCGCGGATAA

Upstream 100 bases:

>100_bases
GCATAAAAAAACAGCAATAAAATGATGGGGGCTTGAAATAGCCCAAATAAACCCTTATATCCATTTCAAATTGATTCACC
GTGCGTATAATGGCGCCATT

Downstream 100 bases:

>100_bases
TGAACAAACGATTGAACTTGCAGCAAATGATACTAGGTTATCGCCACGCTACGAGTTACAATCAACTTTCTTGGGGGCGA
TTCTGGATTCGACAGGATTC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MVKKNSKKAAPATIARNKRATFEYRFEEKMEAGISLMGWEVKSIRMGKVNLSDCYVFLKNGEAFMHGCTIIPLNTASTHV
VCDPIRLKKLLLSRKELDKLAGLVERQGYSIIPISMYWRKGAWVKVEIGLGKGKKDHDKREDTKAREWEVEKARVMKKEK
TRG

Sequences:

>Translated_163_residues
MVKKNSKKAAPATIARNKRATFEYRFEEKMEAGISLMGWEVKSIRMGKVNLSDCYVFLKNGEAFMHGCTIIPLNTASTHV
VCDPIRLKKLLLSRKELDKLAGLVERQGYSIIPISMYWRKGAWVKVEIGLGKGKKDHDKREDTKAREWEVEKARVMKKEK
TRG
>Mature_163_residues
MVKKNSKKAAPATIARNKRATFEYRFEEKMEAGISLMGWEVKSIRMGKVNLSDCYVFLKNGEAFMHGCTIIPLNTASTHV
VCDPIRLKKLLLSRKELDKLAGLVERQGYSIIPISMYWRKGAWVKVEIGLGKGKKDHDKREDTKAREWEVEKARVMKKEK
TRG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=59.8726114649682, Blast_Score=202, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18701; Mature: 18701

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKNSKKAAPATIARNKRATFEYRFEEKMEAGISLMGWEVKSIRMGKVNLSDCYVFLKN
CCCCCCCCCCCHHHHCCCCCEEEEEHHHHHHCCCEEECEEEEEEEECCEEHHHEEEEEEC
GEAFMHGCTIIPLNTASTHVVCDPIRLKKLLLSRKELDKLAGLVERQGYSIIPISMYWRK
CCEEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GAWVKVEIGLGKGKKDHDKREDTKAREWEVEKARVMKKEKTRG
CCEEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKKNSKKAAPATIARNKRATFEYRFEEKMEAGISLMGWEVKSIRMGKVNLSDCYVFLKN
CCCCCCCCCCCHHHHCCCCCEEEEEHHHHHHCCCEEECEEEEEEEECCEEHHHEEEEEEC
GEAFMHGCTIIPLNTASTHVVCDPIRLKKLLLSRKELDKLAGLVERQGYSIIPISMYWRK
CCEEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
GAWVKVEIGLGKGKKDHDKREDTKAREWEVEKARVMKKEKTRG
CCEEEEEEECCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA