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The map label for this gene is tolB [H]

Identifier: 117920972

GI number: 117920972

Start: 3027534

End: 3028862

Strand: Reverse

Name: tolB [H]

Synonym: Shewana3_2531

Alternate gene names: 117920972

Gene position: 3028862-3027534 (Counterclockwise)

Preceding gene: 117920973

Following gene: 117920971

Centisome position: 60.92

GC content: 48.53

Gene sequence:

>1329_bases
ATGAAAATTTTGGCAAAATGGTTAGCATTGGCGGTTCTGCTTTGCACTATGCCGGCAAAAGCGGCGCTGGATATTGTGAT
CACTGAGGGTGTGGATGCGGCGCGTCCAATCGCAGTGATGCCATTCCAATGGCAAGGTGCGGGTGCGCCACCCCAGGCGA
TCGCCGATGTGGTGATGTCTGACTTAATTCGTAGCGGTACCTTCAAACCATTAGATGAATTAGGGTTACCACAACGTGGT
ATCGGTGCCTTAGCCCAGTTTGACGCGAGCGCGTGGGCCAATGTCGGTGCCGAAGCCGTCGTAGTCGGCTCGGTAAAACC
TTATGGTACGGACCAGTTTTTGGTGAGCTTTGACCTTATCGATTTGGTTAAAGCGCAAAACCAAGCGCTAAAAGGCCCTA
CCAGTGCGACAGAGTTTTTGATGGACAGTCGCCAAACAGTGATTTCTGCGGCGCAGTTCCGTCAATATGGCCACCGTATT
AGCGATATCGTCTATGAGAAGCTTACTGGTATTCGTGGCGCTTTCTTAACCCGTATTTCCTACGTGGTGGTGAATCACAC
TCAGAAAGCGGCTTATTCACTGATGATCGCCGACTATGACGGTTACAACGAGCAAATGTTACTGCGCTCTCCTGAGCCGC
TGATGTCGCCTTCTTGGTCACCCGATGGTCGTCGTTTAGCCTATGTAAGCTTTGAAAACAAAAAGGCTGAGATTTTTGTT
CAGGATCTTTACACCCAAGTGCGTACTAAGGTGAGCAGCTTCCCTGGCATTAACGGTGCACCGACTTTCTCGCCAGATGG
TAAATCCTTGGCTGTGACCCTCTCAAAGGATGGTCAACCTGAGATTTACGTTATCGATATCGCCACTAAGGCGGCAAAAC
GTATTACCAACCACTATTCCATCGATACTGAGCCTTCTTGGTATCCCGATGGCAAGTCATTGTTGTTTACCTCCGAGCGC
GGTGGTAGACCACAGTTATATCGTGTAGATTTAAATTCAGGCAAAGTGACCCGTGAAACCTTTGAAGGTGAATGGAATTT
AGGTGGTTCAATTACCCCTGATGGTCGCAGCATGATTTTTGTGAATCGCACAAACGGTAAATTTAATATTGCACGTATGG
ATTTAAGTACGCGCTTTATGCAGGTGCTGACATCGACACGTTTGGACGAATCTCCAAGTGTGGCCCCTAATGGTACTATG
GTGATTTATGGCACCACGTATCAAGGCAAGCAAGTTTTAGCAGCTGTTTCTACGGACGGACGCTTTAAAGCGAGATTACC
GGTAGGTCAGGGCGAGGTGAAATCACCTTCTTGGTCTCCGTTTCTCTAA

Upstream 100 bases:

>100_bases
AAAAGCGGGCAAGTTACCAGTGTCTCCGGATCCTGCTGTTTATAACTTGATGAAAGAAATTAACTTAATCGTTGAACCAA
CGTTTAATTAAAGGAGTCCC

Downstream 100 bases:

>100_bases
TGTATTAGCTCGATTTATTCACATTTAATTTTATAAGGAACAGATAATGGATCTGAACAAGTTGTTAAAAGCTATGTTGG
TAGCACTTCCAATTATGGCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMSDLIRSGTFKPLDELGLPQRG
IGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLIDLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRI
SDIVYEKLTGIRGAFLTRISYVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYSIDTEPSWYPDGKSLLFTSER
GGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIFVNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTM
VIYGTTYQGKQVLAAVSTDGRFKARLPVGQGEVKSPSWSPFL

Sequences:

>Translated_442_residues
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMSDLIRSGTFKPLDELGLPQRG
IGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLIDLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRI
SDIVYEKLTGIRGAFLTRISYVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYSIDTEPSWYPDGKSLLFTSER
GGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIFVNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTM
VIYGTTYQGKQVLAAVSTDGRFKARLPVGQGEVKSPSWSPFL
>Mature_442_residues
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMSDLIRSGTFKPLDELGLPQRG
IGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLIDLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRI
SDIVYEKLTGIRGAFLTRISYVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYSIDTEPSWYPDGKSLLFTSER
GGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIFVNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTM
VIYGTTYQGKQVLAAVSTDGRFKARLPVGQGEVKSPSWSPFL

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=431, Percent_Identity=46.1716937354988, Blast_Score=386, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48359; Mature: 48359

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMS
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLIRSGTFKPLDELGLPQRGIGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLI
HHHHCCCCCCHHHCCCCCCCCHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCEEEEHHHH
DLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRISDIVYEKLTGIRGAFLTRIS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEE
YVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
EEEEECCCHHEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEHH
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHCCC
IDTEPSWYPDGKSLLFTSERGGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIF
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEHEECCCCCCCCCEECCCCCEEEE
VNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSTDG
EECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCC
RFKARLPVGQGEVKSPSWSPFL
EEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILAKWLALAVLLCTMPAKAALDIVITEGVDAARPIAVMPFQWQGAGAPPQAIADVVMS
CHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLIRSGTFKPLDELGLPQRGIGALAQFDASAWANVGAEAVVVGSVKPYGTDQFLVSFDLI
HHHHCCCCCCHHHCCCCCCCCHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCEEEEHHHH
DLVKAQNQALKGPTSATEFLMDSRQTVISAAQFRQYGHRISDIVYEKLTGIRGAFLTRIS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEE
YVVVNHTQKAAYSLMIADYDGYNEQMLLRSPEPLMSPSWSPDGRRLAYVSFENKKAEIFV
EEEEECCCHHEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEHH
QDLYTQVRTKVSSFPGINGAPTFSPDGKSLAVTLSKDGQPEIYVIDIATKAAKRITNHYS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCHHHHHHHHHCCC
IDTEPSWYPDGKSLLFTSERGGRPQLYRVDLNSGKVTRETFEGEWNLGGSITPDGRSMIF
CCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEHEECCCCCCCCCEECCCCCEEEE
VNRTNGKFNIARMDLSTRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSTDG
EECCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCC
RFKARLPVGQGEVKSPSWSPFL
EEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA