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The map label for this gene is emrB [H]

Identifier: 117920667

GI number: 117920667

Start: 2673518

End: 2675098

Strand: Reverse

Name: emrB [H]

Synonym: Shewana3_2224

Alternate gene names: 117920667

Gene position: 2675098-2673518 (Counterclockwise)

Preceding gene: 117920668

Following gene: 117920665

Centisome position: 53.8

GC content: 50.09

Gene sequence:

>1581_bases
GTGAGTCAGCAAGTGATGGCAGGGCAAGACCATGCCCAAGCTGAGGCCGTTAACGCGAGCGGTGCTTTGCAAACCGATAC
CAAAAAGCACGAGCAACATCTTGGGTTTATCACGCTTTCGGTGATGCTAGCGACCATTATGCAGGCGCTGGATACAACGA
TTGCCAACGTGGCCTTGCCCCATATGCAGGGCAGCATGGGGGCGACTCAGGATCAGATCTCTTGGGTGTTAACCTCCTAT
ATTGTGGCCGCGGCGATTTTTATGCCGCTGACGGGTTTTTTAACCGCCAGGATTGGGCGTAAACGGGTATTTATGTGGGC
AGTGGTGGGCTTTACGATTGCCTCTATGCTGTGCGGTGCGGCGCAAAACCTTGAGCAAATTGTGTTATTTCGCTTATTGC
AAGGGGTGTTTGGTGCCAGTTTAGTGCCTTTATCCCAATCGGTATTACTCGACAGTTACCCGCCAGAGCGCCATGGTTCG
GCCATGGCCCTTTGGGGTGTTGGTGTGATGGTCGGGCCGATTTTGGGACCATCCCTCGGTGGTTGGTTAACTGAGTATTA
CAACTGGCGCTGGGTGTTCTATATCAACCTGCCTTTTGGGCTATTGGCTTGGTTTGGTCTTGCCGCCTATGTGAAAGAAA
CGCCGCTGGACCATAGCCGCAAGTTCGATTTACTCGGTTTTGCCATGTTGAGTCTGGCCATTGGCGCCCTGCAAATGTTG
CTCGATAGGGGAGAATCCCTCGATTGGTTTTCCAGTCGTGAAATCGTTATCGAAGCCATAATTGCTGGCATGGCGTTTTA
TCTGTTTATTGCCCATATCTTTACCCATAAACATCCCTTTATTGAGCCTGGGCTGTTTAAGGACAGAAACTTCAGTGTCG
GGCTTATCTTCATTTTTATCATCGGGATTATTCTGCTGGCGACCATGGCCTTATTGCCGCCTTTTATGCAGACCCTGTTA
GGTTATCCTGTGATTGATGTGGGTTACTTGCTGGCGCCAAGGGGCGTCGGCACTATGATCGCCATGATGACTGTGGGTAA
GCTCGCCGGCAAAGTGGATGTACGTTACCAAATCTTTTTAGGTTTGATGCTCACCATTTTGTCCCTATGGGAGATGACGG
GCTTTAACACCAATATTACCGGCTGGGATATAGTGCGAACTGGGGTTATCCAAGGCCTTGGCTTGGGCTTTATCTTTGTG
CCACTCTCGACCATTACCTTTGCAACCTTAGCGGCAAAATACCGTAATGAGGGGACTGCGCTCTTTAGTCTGATGCGAAA
TATCGGCAGCAGTATAGGGATCTCCGTGGTGATCACCTATCTTGCCCAGCACACACAGATGAATCATGCGGCGTTTGCCG
ATTACATTCATCCCTTTAGTTTTGCGCTCAAGCACGCCGTTGATCTTCATGCCGTTGATTTATCGACGACACAGGGCATA
GCCTTACTCAATGCCGAAGTGAATCGTCAGGCGGCGACCTTGGCCTATTTGCAGGACTTTAGGTTAATGATGTGGGTGAC
GTTATCCGCCGTGCCCTTAGTGTTTCTGCTTAAAGGCGTGCATAAAAAACCAAAGCCATAG

Upstream 100 bases:

>100_bases
CCATTGATACCGCGCTGCCCGAGGCGCCGCTGCGCGCCGGATTAAGCGCCGTGGTGGATATCGATACTGAATATCAACGT
CAGTTGTTGGGTTTTAGTCT

Downstream 100 bases:

>100_bases
GACGAGTATTCGTCGGAGTTGAGGGCATCACGCTAGCGATAAATGCATGATAAATAGAAAAACGCCGCATCTTTTGAGTG
CGGCGTTTTTTATTGTGTCC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSQQVMAGQDHAQAEAVNASGALQTDTKKHEQHLGFITLSVMLATIMQALDTTIANVALPHMQGSMGATQDQISWVLTSY
IVAAAIFMPLTGFLTARIGRKRVFMWAVVGFTIASMLCGAAQNLEQIVLFRLLQGVFGASLVPLSQSVLLDSYPPERHGS
AMALWGVGVMVGPILGPSLGGWLTEYYNWRWVFYINLPFGLLAWFGLAAYVKETPLDHSRKFDLLGFAMLSLAIGALQML
LDRGESLDWFSSREIVIEAIIAGMAFYLFIAHIFTHKHPFIEPGLFKDRNFSVGLIFIFIIGIILLATMALLPPFMQTLL
GYPVIDVGYLLAPRGVGTMIAMMTVGKLAGKVDVRYQIFLGLMLTILSLWEMTGFNTNITGWDIVRTGVIQGLGLGFIFV
PLSTITFATLAAKYRNEGTALFSLMRNIGSSIGISVVITYLAQHTQMNHAAFADYIHPFSFALKHAVDLHAVDLSTTQGI
ALLNAEVNRQAATLAYLQDFRLMMWVTLSAVPLVFLLKGVHKKPKP

Sequences:

>Translated_526_residues
MSQQVMAGQDHAQAEAVNASGALQTDTKKHEQHLGFITLSVMLATIMQALDTTIANVALPHMQGSMGATQDQISWVLTSY
IVAAAIFMPLTGFLTARIGRKRVFMWAVVGFTIASMLCGAAQNLEQIVLFRLLQGVFGASLVPLSQSVLLDSYPPERHGS
AMALWGVGVMVGPILGPSLGGWLTEYYNWRWVFYINLPFGLLAWFGLAAYVKETPLDHSRKFDLLGFAMLSLAIGALQML
LDRGESLDWFSSREIVIEAIIAGMAFYLFIAHIFTHKHPFIEPGLFKDRNFSVGLIFIFIIGIILLATMALLPPFMQTLL
GYPVIDVGYLLAPRGVGTMIAMMTVGKLAGKVDVRYQIFLGLMLTILSLWEMTGFNTNITGWDIVRTGVIQGLGLGFIFV
PLSTITFATLAAKYRNEGTALFSLMRNIGSSIGISVVITYLAQHTQMNHAAFADYIHPFSFALKHAVDLHAVDLSTTQGI
ALLNAEVNRQAATLAYLQDFRLMMWVTLSAVPLVFLLKGVHKKPKP
>Mature_525_residues
SQQVMAGQDHAQAEAVNASGALQTDTKKHEQHLGFITLSVMLATIMQALDTTIANVALPHMQGSMGATQDQISWVLTSYI
VAAAIFMPLTGFLTARIGRKRVFMWAVVGFTIASMLCGAAQNLEQIVLFRLLQGVFGASLVPLSQSVLLDSYPPERHGSA
MALWGVGVMVGPILGPSLGGWLTEYYNWRWVFYINLPFGLLAWFGLAAYVKETPLDHSRKFDLLGFAMLSLAIGALQMLL
DRGESLDWFSSREIVIEAIIAGMAFYLFIAHIFTHKHPFIEPGLFKDRNFSVGLIFIFIIGIILLATMALLPPFMQTLLG
YPVIDVGYLLAPRGVGTMIAMMTVGKLAGKVDVRYQIFLGLMLTILSLWEMTGFNTNITGWDIVRTGVIQGLGLGFIFVP
LSTITFATLAAKYRNEGTALFSLMRNIGSSIGISVVITYLAQHTQMNHAAFADYIHPFSFALKHAVDLHAVDLSTTQGIA
LLNAEVNRQAATLAYLQDFRLMMWVTLSAVPLVFLLKGVHKKPKP

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=440, Percent_Identity=35.9090909090909, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788710, Length=469, Percent_Identity=31.9829424307036, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1790195, Length=440, Percent_Identity=28.6363636363636, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788392, Length=395, Percent_Identity=31.3924050632911, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081983, Length=429, Percent_Identity=26.8065268065268, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87082320, Length=196, Percent_Identity=23.469387755102, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1787065, Length=196, Percent_Identity=27.0408163265306, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=382, Percent_Identity=25.6544502617801, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=150, Percent_Identity=30.6666666666667, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=180, Percent_Identity=28.8888888888889, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=202, Percent_Identity=26.2376237623762, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=454, Percent_Identity=20.9251101321586, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323000, Length=121, Percent_Identity=29.7520661157025, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57742; Mature: 57611

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQQVMAGQDHAQAEAVNASGALQTDTKKHEQHLGFITLSVMLATIMQALDTTIANVALP
CCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]