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The map label for this gene is emrA [H]

Identifier: 117920668

GI number: 117920668

Start: 2675095

End: 2676150

Strand: Reverse

Name: emrA [H]

Synonym: Shewana3_2225

Alternate gene names: 117920668

Gene position: 2676150-2675095 (Counterclockwise)

Preceding gene: 117920669

Following gene: 117920667

Centisome position: 53.82

GC content: 51.04

Gene sequence:

>1056_bases
ATGAGTGACGCAAATCCTCAAGCACCTAAAGCGAATACGCGTAAAAAGCGCGGCATATTACTGATTGTGGTGCCGCTGTT
ATCCGTGCTGGCCATAGGTGCGGTTTACCTGCATGGCGGCCGTTATATGGAAACCGATAACGCCTATGTAAAGGCGGATA
AAGTGCCCGTAAGCGCGCAAGTGGCTGGGAATGTCGACAGCTTGTATGTGGTTGAAAACCAAAGGGTTGAGAAGGGGAAA
GTGTTGTTTCGCCTCGACGATGCCATGTTTAAGGTGATGGTCGATAAGGCGAGTGCCAAATTGGCACAGGTCAAGACGGA
TTTAGCGGTACTCAAGGCGAGTTACCATGAGAAGCAAGCCGAAATCACTCTGGCAGAGACCAAGTTAGCCTTTGCGGAGA
AAGAGCAAAAGCGGCAGGAGAATTTGATCGGTAAACACTTTGTGTCTGAATCTCAGCTTGAAGATGCAAGGCAAAATACC
GATATTGCGCGGCAGAATATTCAGACCCTGCAAAAAGACTTACACCGCATCGCCGAAAGCTTAGGCGGCAGCCCCGATTT
TCCGATTGAACAACATCCCAGCTATTTAGAAGCCTTAGCCCAATTAAATGAAGCCAAGCTTGATTTAAGCCGCGTGGAGA
TTAAAGCCCCAGTATCAGGCGTCGTCAGCCAACTGCCAAAGCTAGGACAATATGTGAATGTTGGCGCCATTGCCCTCGCG
CTGGTAGCCGATCATGCCCTCTGGATTGAGGCGAACTTCACCGAAACCGATTTAACCCACGTCAAACCCGGGCAAAAGGT
CAATATCCATATCGATACCTTTCCCGATAATCACTGGCAAGGTACGGTCGAGAGTTTAAGTCCCGCAACGGGTGCAGAGT
TCTCGCTGATCCCCGCGCAAAATGCAACCGGCAACTGGGTGAAAATCGCCCAGCGCGTTCCGGTGCGAATTGCCATTGAT
ACCGCGCTGCCCGAGGCGCCGCTGCGCGCCGGATTAAGCGCCGTGGTGGATATCGATACTGAATATCAACGTCAGTTGTT
GGGTTTTAGTCTGTGA

Upstream 100 bases:

>100_bases
TATCCGGCTGTTATCGCAAATCCATAAAAATTTTTCAGCTTAATCAAAGCAGATAAACCCATCCCCGTGATAGACATTTA
GTGAAGTTGGAGTTGTTGTA

Downstream 100 bases:

>100_bases
GTCAGCAAGTGATGGCAGGGCAAGACCATGCCCAAGCTGAGGCCGTTAACGCGAGCGGTGCTTTGCAAACCGATACCAAA
AAGCACGAGCAACATCTTGG

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSDANPQAPKANTRKKRGILLIVVPLLSVLAIGAVYLHGGRYMETDNAYVKADKVPVSAQVAGNVDSLYVVENQRVEKGK
VLFRLDDAMFKVMVDKASAKLAQVKTDLAVLKASYHEKQAEITLAETKLAFAEKEQKRQENLIGKHFVSESQLEDARQNT
DIARQNIQTLQKDLHRIAESLGGSPDFPIEQHPSYLEALAQLNEAKLDLSRVEIKAPVSGVVSQLPKLGQYVNVGAIALA
LVADHALWIEANFTETDLTHVKPGQKVNIHIDTFPDNHWQGTVESLSPATGAEFSLIPAQNATGNWVKIAQRVPVRIAID
TALPEAPLRAGLSAVVDIDTEYQRQLLGFSL

Sequences:

>Translated_351_residues
MSDANPQAPKANTRKKRGILLIVVPLLSVLAIGAVYLHGGRYMETDNAYVKADKVPVSAQVAGNVDSLYVVENQRVEKGK
VLFRLDDAMFKVMVDKASAKLAQVKTDLAVLKASYHEKQAEITLAETKLAFAEKEQKRQENLIGKHFVSESQLEDARQNT
DIARQNIQTLQKDLHRIAESLGGSPDFPIEQHPSYLEALAQLNEAKLDLSRVEIKAPVSGVVSQLPKLGQYVNVGAIALA
LVADHALWIEANFTETDLTHVKPGQKVNIHIDTFPDNHWQGTVESLSPATGAEFSLIPAQNATGNWVKIAQRVPVRIAID
TALPEAPLRAGLSAVVDIDTEYQRQLLGFSL
>Mature_350_residues
SDANPQAPKANTRKKRGILLIVVPLLSVLAIGAVYLHGGRYMETDNAYVKADKVPVSAQVAGNVDSLYVVENQRVEKGKV
LFRLDDAMFKVMVDKASAKLAQVKTDLAVLKASYHEKQAEITLAETKLAFAEKEQKRQENLIGKHFVSESQLEDARQNTD
IARQNIQTLQKDLHRIAESLGGSPDFPIEQHPSYLEALAQLNEAKLDLSRVEIKAPVSGVVSQLPKLGQYVNVGAIALAL
VADHALWIEANFTETDLTHVKPGQKVNIHIDTFPDNHWQGTVESLSPATGAEFSLIPAQNATGNWVKIAQRVPVRIAIDT
ALPEAPLRAGLSAVVDIDTEYQRQLLGFSL

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=348, Percent_Identity=32.7586206896552, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789041, Length=342, Percent_Identity=32.1637426900585, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI87081951, Length=323, Percent_Identity=27.5541795665635, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790519, Length=310, Percent_Identity=28.3870967741935, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789637, Length=326, Percent_Identity=26.3803680981595, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790024, Length=275, Percent_Identity=27.6363636363636, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790012, Length=271, Percent_Identity=26.5682656826568, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787013, Length=250, Percent_Identity=28.8, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081796, Length=239, Percent_Identity=26.3598326359833, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38461; Mature: 38330

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDANPQAPKANTRKKRGILLIVVPLLSVLAIGAVYLHGGRYMETDNAYVKADKVPVSAQ
CCCCCCCCCCCCCHHHCCEEEEHHHHHHHHHHHHHHEECCEEEECCCCEEEECCCCEEEE
VAGNVDSLYVVENQRVEKGKVLFRLDDAMFKVMVDKASAKLAQVKTDLAVLKASYHEKQA
ECCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EITLAETKLAFAEKEQKRQENLIGKHFVSESQLEDARQNTDIARQNIQTLQKDLHRIAES
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGSPDFPIEQHPSYLEALAQLNEAKLDLSRVEIKAPVSGVVSQLPKLGQYVNVGAIALA
HCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEECCHHHHHHHHHHHCCHHHHHHHHHH
LVADHALWIEANFTETDLTHVKPGQKVNIHIDTFPDNHWQGTVESLSPATGAEFSLIPAQ
HHHCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEECC
NATGNWVKIAQRVPVRIAIDTALPEAPLRAGLSAVVDIDTEYQRQLLGFSL
CCCCCHHHHHHCCCEEEEEECCCCCHHHHCCCEEEEECCHHHHHHHHCCCC
>Mature Secondary Structure 
SDANPQAPKANTRKKRGILLIVVPLLSVLAIGAVYLHGGRYMETDNAYVKADKVPVSAQ
CCCCCCCCCCCCHHHCCEEEEHHHHHHHHHHHHHHEECCEEEECCCCEEEECCCCEEEE
VAGNVDSLYVVENQRVEKGKVLFRLDDAMFKVMVDKASAKLAQVKTDLAVLKASYHEKQA
ECCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EITLAETKLAFAEKEQKRQENLIGKHFVSESQLEDARQNTDIARQNIQTLQKDLHRIAES
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGSPDFPIEQHPSYLEALAQLNEAKLDLSRVEIKAPVSGVVSQLPKLGQYVNVGAIALA
HCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEEEECCHHHHHHHHHHHCCHHHHHHHHHH
LVADHALWIEANFTETDLTHVKPGQKVNIHIDTFPDNHWQGTVESLSPATGAEFSLIPAQ
HHHCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEECC
NATGNWVKIAQRVPVRIAIDTALPEAPLRAGLSAVVDIDTEYQRQLLGFSL
CCCCCHHHHHHCCCEEEEEECCCCCHHHHCCCEEEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]