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The map label for this gene is thiE [H]

Identifier: 117920382

GI number: 117920382

Start: 2304011

End: 2305693

Strand: Direct

Name: thiE [H]

Synonym: Shewana3_1937

Alternate gene names: 117920382

Gene position: 2304011-2305693 (Clockwise)

Preceding gene: 117920381

Following gene: 117920383

Centisome position: 46.34

GC content: 50.86

Gene sequence:

>1683_bases
ATGACCTCTGCTAAACATGTTAGCCACTTGGCGCCTAAACCTATCGTCTGGACCATTGCGGGTTCAGACAGCGGTGGCGG
CGCGGGGATCCAAGCCGACTTAGCCACCATCAAGGATTTGGGCGGTCATGGATGCAGCGTGATCACCACGTTAACGGCCC
AAAGTTCGGTGGCGGTGGATTTAGTTGAGCCTGTGAGTGAGGCAATGCTACTCACACAGCTCTCGACCCTGTTAGCCGAT
CTACCGCCTCAGGCGATTAAAATTGGCTTACTCGCGAATCAGCAGCAACTGCATTTAGTTGCCGATTGGTTGGCTGGCTT
TAAAACCCAGTTTCCACTCGTCCCTGTTATTCTCGACCCTGTAATGGTTGCAAGCTGTGGGGATGAACTTGGGGATAAGA
GCACGGCGAGCCAGCCACTGGATTTTACGCCCTTTAAGGGCTTGATTAGCCTGATAACGCCGAATGTGCAGGAGTTGGCA
AAGCTTACTGTCACAACTGACAAACAAGTGTCACCAATACACACAAAAGCAGCGTTCGCTGCTGCGGCAATGCAACTCTC
TGAACAATTAGACTGCAGCGTATTAGCCAAGGGCGGCGATGTTGATTTTGCGGCTCAGGCAAGCGTAGGCATTAATACAA
GGGATAGCTTAAGCGGTCACAAAAGCGATATCACAAGTCATCGCAGTGCCACAGATAATCAGCGTATGGCGGAAGATTTG
TTGATTTGTCATCAGGTTACAGGTTGCACACCGCTCGACGCTAATGGCTGCTTTTGGCTCAGCAGTGCGCGGATTAACAC
GCGCCATAACCACGGCAGTGGCTGTACTCTGTCTTCGGCCATCGCCTCGGTGTTAGCCTTTGGCTTTGTATTGCAGGATG
CCGTTGTGGTAGCAAAAGCCTATGTCAATCAAGGCTTAACTTATGCAGGGGGGATTGGCCAAGGCCCAGGGCCACTCGCG
CGTACCGCTTGGCCGCACAATTTGACGGCGTATCCTCATATTACTGCTTATTCTGAAAACAGCTTGAGTGAATCCAGTGA
TGTGCAATGCGGCGCGTTTAAGCGCCTTGAGCCTGACTTAGGAATCTATCCTGTCGTCGATAACCTACTGTTACTCGAAC
AGTTATTAGCGGCAGGCGTGAAGACGGTACAGCTCAGGATAAAGTCTAATGCGCTAAAGTCGGACGAACTTGAGGCTCAA
ATCCAAACCGCGATTGCCTTAGGTAAACACTATGAAGCGCAGCTTTTTATCAATGATCATTGGCAGTTAGCGATAAAACA
TGGCGCATTTGGGGTGCATCTTGGCCAAGAAGATCTGGCAGTGACGGATCTTAATGCCATTCATGCAGCAGGATTGGCGC
TAGGCATCTCGAGCCACGGTTATTTCGAGTTGCTGCGTGCCCATCAACATGCGCCATCTTACATCGCCCTCGGGCATATT
TTCCCAACGACCACCAAGCAAATGCCATCGGCGCCGCAGGGATTATTTAAACTCACTCATTATGTTGAGCTGTTAAATAC
ACACTATCCCTTAGTGGCAATTGGTGGCATAGGACCTTCGAATCTTTTGCTAGTCAAAGCGACTGGGGTGAGCAATATTG
CCGTGGTGCGGGCGATTACCGAGGCTAATGATCCAGTAATGGCCCTTGCCGAATTGACGCGAGCCTGGGAGTCAAGCCTA
TGA

Upstream 100 bases:

>100_bases
CGCTAGGTGATGCTAATGCAGCCAATACTGCCAACGAATCGGCAAAGCATCAGGATGGGACAGAGCTCCAACCCACAGTA
CAGGTAGCGGAGGTTTGATA

Downstream 100 bases:

>100_bases
TCACGACACAAGAGCAGGCGCAATCCCTTGCCGATAAAGACTTTATTCGTTATTCGCGGCAAATATTTTTACCCGAGGTC
GGCGAGGCTGGCCAGTTGCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTSAKHVSHLAPKPIVWTIAGSDSGGGAGIQADLATIKDLGGHGCSVITTLTAQSSVAVDLVEPVSEAMLLTQLSTLLAD
LPPQAIKIGLLANQQQLHLVADWLAGFKTQFPLVPVILDPVMVASCGDELGDKSTASQPLDFTPFKGLISLITPNVQELA
KLTVTTDKQVSPIHTKAAFAAAAMQLSEQLDCSVLAKGGDVDFAAQASVGINTRDSLSGHKSDITSHRSATDNQRMAEDL
LICHQVTGCTPLDANGCFWLSSARINTRHNHGSGCTLSSAIASVLAFGFVLQDAVVVAKAYVNQGLTYAGGIGQGPGPLA
RTAWPHNLTAYPHITAYSENSLSESSDVQCGAFKRLEPDLGIYPVVDNLLLLEQLLAAGVKTVQLRIKSNALKSDELEAQ
IQTAIALGKHYEAQLFINDHWQLAIKHGAFGVHLGQEDLAVTDLNAIHAAGLALGISSHGYFELLRAHQHAPSYIALGHI
FPTTTKQMPSAPQGLFKLTHYVELLNTHYPLVAIGGIGPSNLLLVKATGVSNIAVVRAITEANDPVMALAELTRAWESSL

Sequences:

>Translated_560_residues
MTSAKHVSHLAPKPIVWTIAGSDSGGGAGIQADLATIKDLGGHGCSVITTLTAQSSVAVDLVEPVSEAMLLTQLSTLLAD
LPPQAIKIGLLANQQQLHLVADWLAGFKTQFPLVPVILDPVMVASCGDELGDKSTASQPLDFTPFKGLISLITPNVQELA
KLTVTTDKQVSPIHTKAAFAAAAMQLSEQLDCSVLAKGGDVDFAAQASVGINTRDSLSGHKSDITSHRSATDNQRMAEDL
LICHQVTGCTPLDANGCFWLSSARINTRHNHGSGCTLSSAIASVLAFGFVLQDAVVVAKAYVNQGLTYAGGIGQGPGPLA
RTAWPHNLTAYPHITAYSENSLSESSDVQCGAFKRLEPDLGIYPVVDNLLLLEQLLAAGVKTVQLRIKSNALKSDELEAQ
IQTAIALGKHYEAQLFINDHWQLAIKHGAFGVHLGQEDLAVTDLNAIHAAGLALGISSHGYFELLRAHQHAPSYIALGHI
FPTTTKQMPSAPQGLFKLTHYVELLNTHYPLVAIGGIGPSNLLLVKATGVSNIAVVRAITEANDPVMALAELTRAWESSL
>Mature_559_residues
TSAKHVSHLAPKPIVWTIAGSDSGGGAGIQADLATIKDLGGHGCSVITTLTAQSSVAVDLVEPVSEAMLLTQLSTLLADL
PPQAIKIGLLANQQQLHLVADWLAGFKTQFPLVPVILDPVMVASCGDELGDKSTASQPLDFTPFKGLISLITPNVQELAK
LTVTTDKQVSPIHTKAAFAAAAMQLSEQLDCSVLAKGGDVDFAAQASVGINTRDSLSGHKSDITSHRSATDNQRMAEDLL
ICHQVTGCTPLDANGCFWLSSARINTRHNHGSGCTLSSAIASVLAFGFVLQDAVVVAKAYVNQGLTYAGGIGQGPGPLAR
TAWPHNLTAYPHITAYSENSLSESSDVQCGAFKRLEPDLGIYPVVDNLLLLEQLLAAGVKTVQLRIKSNALKSDELEAQI
QTAIALGKHYEAQLFINDHWQLAIKHGAFGVHLGQEDLAVTDLNAIHAAGLALGISSHGYFELLRAHQHAPSYIALGHIF
PTTTKQMPSAPQGLFKLTHYVELLNTHYPLVAIGGIGPSNLLLVKATGVSNIAVVRAITEANDPVMALAELTRAWESSL

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=192, Percent_Identity=60.4166666666667, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1788420, Length=312, Percent_Identity=31.4102564102564, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325378, Length=295, Percent_Identity=29.1525423728814, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324997, Length=190, Percent_Identity=30.5263157894737, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=193, Percent_Identity=30.0518134715026, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324517, Length=190, Percent_Identity=30, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 59145; Mature: 59014

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKHVSHLAPKPIVWTIAGSDSGGGAGIQADLATIKDLGGHGCSVITTLTAQSSVAVD
CCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEHH
LVEPVSEAMLLTQLSTLLADLPPQAIKIGLLANQQQLHLVADWLAGFKTQFPLVPVILDP
HHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHH
VMVASCGDELGDKSTASQPLDFTPFKGLISLITPNVQELAKLTVTTDKQVSPIHTKAAFA
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHEEECCCCCCCCCHHHHHHH
AAAMQLSEQLDCSVLAKGGDVDFAAQASVGINTRDSLSGHKSDITSHRSATDNQRMAEDL
HHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
LICHQVTGCTPLDANGCFWLSSARINTRHNHGSGCTLSSAIASVLAFGFVLQDAVVVAKA
HHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVNQGLTYAGGIGQGPGPLARTAWPHNLTAYPHITAYSENSLSESSDVQCGAFKRLEPDL
HHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCC
GIYPVVDNLLLLEQLLAAGVKTVQLRIKSNALKSDELEAQIQTAIALGKHYEAQLFINDH
CCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
WQLAIKHGAFGVHLGQEDLAVTDLNAIHAAGLALGISSHGYFELLRAHQHAPSYIALGHI
EEEEEECCCEEEEECCCCCEEECHHHHHHHHHEEECCCCCHHHHHHHHCCCCCEEEEECC
FPTTTKQMPSAPQGLFKLTHYVELLNTHYPLVAIGGIGPSNLLLVKATGVSNIAVVRAIT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEC
EANDPVMALAELTRAWESSL
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSAKHVSHLAPKPIVWTIAGSDSGGGAGIQADLATIKDLGGHGCSVITTLTAQSSVAVD
CCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEHH
LVEPVSEAMLLTQLSTLLADLPPQAIKIGLLANQQQLHLVADWLAGFKTQFPLVPVILDP
HHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHH
VMVASCGDELGDKSTASQPLDFTPFKGLISLITPNVQELAKLTVTTDKQVSPIHTKAAFA
HHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHEEECCCCCCCCCHHHHHHH
AAAMQLSEQLDCSVLAKGGDVDFAAQASVGINTRDSLSGHKSDITSHRSATDNQRMAEDL
HHHHHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
LICHQVTGCTPLDANGCFWLSSARINTRHNHGSGCTLSSAIASVLAFGFVLQDAVVVAKA
HHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVNQGLTYAGGIGQGPGPLARTAWPHNLTAYPHITAYSENSLSESSDVQCGAFKRLEPDL
HHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCCC
GIYPVVDNLLLLEQLLAAGVKTVQLRIKSNALKSDELEAQIQTAIALGKHYEAQLFINDH
CCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCC
WQLAIKHGAFGVHLGQEDLAVTDLNAIHAAGLALGISSHGYFELLRAHQHAPSYIALGHI
EEEEEECCCEEEEECCCCCEEECHHHHHHHHHEEECCCCCHHHHHHHHCCCCCEEEEECC
FPTTTKQMPSAPQGLFKLTHYVELLNTHYPLVAIGGIGPSNLLLVKATGVSNIAVVRAIT
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEC
EANDPVMALAELTRAWESSL
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA