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The map label for this gene is thiF [H]

Identifier: 117920383

GI number: 117920383

Start: 2305690

End: 2306706

Strand: Direct

Name: thiF [H]

Synonym: Shewana3_1938

Alternate gene names: 117920383

Gene position: 2305690-2306706 (Clockwise)

Preceding gene: 117920382

Following gene: 117920384

Centisome position: 46.37

GC content: 49.16

Gene sequence:

>1017_bases
ATGATCACGACACAAGAGCAGGCGCAATCCCTTGCCGATAAAGACTTTATTCGTTATTCGCGGCAAATATTTTTACCCGA
GGTCGGCGAGGCTGGCCAGTTGCAGCTTAAACAGGCTCATGTGCTTATCGTAGGCCTCGGAGGCCTTGGGCAATTGGCGG
CGCAGTATTTAGCCTGCGCCGGTGTAGGACGCTTAACGCTTGTCGACAGCGATAGGGTGGAATTATCCAATCTGCCAAGG
CAATTACTCTTTAGTGACGACGATATTGGTCATCACAAAGCCTTAATTGCTAAACAGAAATTGGCTGTGTTGGCGCAAGA
TTGTGAGATTGATGCCGTTGCTGAGCATCTGACTATTGAGTCTGCGAACGCGGTATTTTCGAATCTGTTAGCCCATGGCA
AAGAAGCTAGGTTAGTACTTGATTGTACAGATAATCTTGCGGCGCGCCATTGTATCAATCGGCTGTGTATTGAGCATGCC
TTACCTCTGGTGAGCGCATCAATTGCCGCTTTCAACGGCCAATTGTTTGCCGTTGATCAGCAGCGTTTCCCTCAGGGAGG
CTGTTATCACTGTATTTTCCCCTCAGATACTCGCGCGCCACAAAACTGTAGCAGCCAAGGTGTACTTGGCCCCAGTGTCG
GCGTAATGGCATCGATGCAGGCGCTGCTAGCAATGCAATTGTTGCTCCTAGCCACGCCGTCAACCTCTGCAACAAAGCAA
ACAGCCAATCAGACTTTCGAAGAGGCAAGCCAACCAATAGCCTCCACGAGAGCGCTACTTGGAAGATTTTGGCGCTTCGA
TGCCAAGTTGCTGCAATGGCATTCGGCCGCATTAACCCGTGATCCCGAGTGCGAAGTGTGCGGATTAAAACCGAGTTCAT
TAAAGCCGAATTTATTAAAGTCGAGTGAATTAATGCCATGCGAACCAAGCAAGAGCGAAGAAAGGGCTAATGCTAACACC
CACCATTCAACTACAGCCGTTTCATTCAAATCTGTCCCTAATCGAGGTTTGTTATGA

Upstream 100 bases:

>100_bases
CGACTGGGGTGAGCAATATTGCCGTGGTGCGGGCGATTACCGAGGCTAATGATCCAGTAATGGCCCTTGCCGAATTGACG
CGAGCCTGGGAGTCAAGCCT

Downstream 100 bases:

>100_bases
TTTTAATTCATCTCAATAATGTGCCCCAGATACTTCAACAACCGCTGAGTTTACAAGCCCTGTTAGAAGCACAGAATATT
TGCCAACACTCGGTTGCCTT

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MITTQEQAQSLADKDFIRYSRQIFLPEVGEAGQLQLKQAHVLIVGLGGLGQLAAQYLACAGVGRLTLVDSDRVELSNLPR
QLLFSDDDIGHHKALIAKQKLAVLAQDCEIDAVAEHLTIESANAVFSNLLAHGKEARLVLDCTDNLAARHCINRLCIEHA
LPLVSASIAAFNGQLFAVDQQRFPQGGCYHCIFPSDTRAPQNCSSQGVLGPSVGVMASMQALLAMQLLLLATPSTSATKQ
TANQTFEEASQPIASTRALLGRFWRFDAKLLQWHSAALTRDPECEVCGLKPSSLKPNLLKSSELMPCEPSKSEERANANT
HHSTTAVSFKSVPNRGLL

Sequences:

>Translated_338_residues
MITTQEQAQSLADKDFIRYSRQIFLPEVGEAGQLQLKQAHVLIVGLGGLGQLAAQYLACAGVGRLTLVDSDRVELSNLPR
QLLFSDDDIGHHKALIAKQKLAVLAQDCEIDAVAEHLTIESANAVFSNLLAHGKEARLVLDCTDNLAARHCINRLCIEHA
LPLVSASIAAFNGQLFAVDQQRFPQGGCYHCIFPSDTRAPQNCSSQGVLGPSVGVMASMQALLAMQLLLLATPSTSATKQ
TANQTFEEASQPIASTRALLGRFWRFDAKLLQWHSAALTRDPECEVCGLKPSSLKPNLLKSSELMPCEPSKSEERANANT
HHSTTAVSFKSVPNRGLL
>Mature_338_residues
MITTQEQAQSLADKDFIRYSRQIFLPEVGEAGQLQLKQAHVLIVGLGGLGQLAAQYLACAGVGRLTLVDSDRVELSNLPR
QLLFSDDDIGHHKALIAKQKLAVLAQDCEIDAVAEHLTIESANAVFSNLLAHGKEARLVLDCTDNLAARHCINRLCIEHA
LPLVSASIAAFNGQLFAVDQQRFPQGGCYHCIFPSDTRAPQNCSSQGVLGPSVGVMASMQALLAMQLLLLATPSTSATKQ
TANQTFEEASQPIASTRALLGRFWRFDAKLLQWHSAALTRDPECEVCGLKPSSLKPNLLKSSELMPCEPSKSEERANANT
HHSTTAVSFKSVPNRGLL

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=231, Percent_Identity=41.991341991342, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4885649, Length=153, Percent_Identity=30.0653594771242, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI87082356, Length=279, Percent_Identity=40.5017921146953, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787048, Length=281, Percent_Identity=36.6548042704626, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789177, Length=147, Percent_Identity=27.891156462585, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=303, Percent_Identity=35.3135313531353, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI193203301, Length=159, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI86565431, Length=138, Percent_Identity=28.9855072463768, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI86565433, Length=138, Percent_Identity=28.9855072463768, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=226, Percent_Identity=36.283185840708, Blast_Score=125, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24582879, Length=284, Percent_Identity=36.9718309859155, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24660640, Length=169, Percent_Identity=31.3609467455621, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 36561; Mature: 36561

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTQEQAQSLADKDFIRYSRQIFLPEVGEAGQLQLKQAHVLIVGLGGLGQLAAQYLACA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHC
GVGRLTLVDSDRVELSNLPRQLLFSDDDIGHHKALIAKQKLAVLAQDCEIDAVAEHLTIE
CCCEEEEECCCCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SANAVFSNLLAHGKEARLVLDCTDNLAARHCINRLCIEHALPLVSASIAAFNGQLFAVDQ
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECH
QRFPQGGCYHCIFPSDTRAPQNCSSQGVLGPSVGVMASMQALLAMQLLLLATPSTSATKQ
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TANQTFEEASQPIASTRALLGRFWRFDAKLLQWHSAALTRDPECEVCGLKPSSLKPNLLK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHC
SSELMPCEPSKSEERANANTHHSTTAVSFKSVPNRGLL
CCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MITTQEQAQSLADKDFIRYSRQIFLPEVGEAGQLQLKQAHVLIVGLGGLGQLAAQYLACA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHC
GVGRLTLVDSDRVELSNLPRQLLFSDDDIGHHKALIAKQKLAVLAQDCEIDAVAEHLTIE
CCCEEEEECCCCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
SANAVFSNLLAHGKEARLVLDCTDNLAARHCINRLCIEHALPLVSASIAAFNGQLFAVDQ
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECH
QRFPQGGCYHCIFPSDTRAPQNCSSQGVLGPSVGVMASMQALLAMQLLLLATPSTSATKQ
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TANQTFEEASQPIASTRALLGRFWRFDAKLLQWHSAALTRDPECEVCGLKPSSLKPNLLK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHC
SSELMPCEPSKSEERANANTHHSTTAVSFKSVPNRGLL
CCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]