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The map label for this gene is thiC [H]

Identifier: 117920381

GI number: 117920381

Start: 2301846

End: 2304008

Strand: Direct

Name: thiC [H]

Synonym: Shewana3_1936

Alternate gene names: 117920381

Gene position: 2301846-2304008 (Clockwise)

Preceding gene: 117920380

Following gene: 117920382

Centisome position: 46.29

GC content: 52.38

Gene sequence:

>2163_bases
ATGTCCAGTTTAGAATCGTCCTCAGAATCGATTGCCGTGAACACCCTAGGGCAAACGAGCCGCCGTGCGGCGCGCGCCCA
GGCCCAAGCCTTTATCGATACCCTTAAACCATTACAACACCCTAATTCGCAAAAGTTGTATTTGCAGGGGAGTCGTGAGG
ATCTCCGTGTAGGGATGCGGCAAATTCTGCAAACCGATACGCGGATTGGCGGCACTGCAGCAGAGCCTACAGTTGAGAAA
AATCCTCCCATTCCTGTGTACGACTGCGCGGGGCCTTATTCCGATCCAGCTGCGCAAATTAACGTGCGCCAAGGCTTAGC
CAAACTCAGATTACCTTGGATTATCGAGCGCCAAGATACCGAAGTGCTTGATTGTGCGACCTCAGATTTCACCCAGCAGC
GCCTGAAGGATGATGGGCTTGATCATCTGCGTTTCGAAGCCGGTTCAAGTGCAATTGTTCGTCCCCGCCGTGCCTTACCG
GGTAAACGCGTGAGCCAATTACACTATGCCCGCCAAGGGATCATTACCCCCGAAATGGAGTATGTGGCGATTCGGGAAAA
TATGGCGCTCGCCGAGGTGCAGGATGAAATCTTAAACCGCCGCGGCCAAGGTGAATCCTTTGGTACCGTGATCGGTAAAT
CGATTACCCCTGAATTTGTCCGCGACGAAATTGCCCGTGGGCGCGCCATTATTCCGTTAAATATCAATCACCCTGAAGCT
GAGCCCATGATTATTGGCCGTAACTTTCTGGTGAAAGTGAATGCCAATATCGGCAACTCGGCGGTCACGTCTTCCATTGA
GGAGGAAGTGGAAAAGTTAGTCTGGTCAACCCGCTGGGGCGCGGACACTGTGATGGACTTGTCCACGGGGCGATATATCC
ATGAAACCCGTGAATGGATCATCCGAAACTCGCCAGTGCCGATTGGCACAGTGCCGATTTATCAAGCTTTGGAAAAAGTA
AACGGTGTTGCCGAGCACCTGACCTGGGAAGTATTTCGCGATACCTTGATTGAGCAAGCGGAGCAGGGCGTCGATTACTT
TACCATCCATGCGGGCGTGTTATTGCGCTATGTGCCCATGACTGCCAAACGGGTGACTGGGATTGTGTCCCGCGGCGGGT
CGATTATGGCCAAATGGTGTTTGAGTCACCATCAAGAAAACTTCCTCTATACCCATTTCCGCGAGATTTGCGAACTGTGT
GTTGCCTACGATGTATCGCTGTCATTAGGTGACGGTATGCGTCCAGGCTCCATTGCCGATGCCAATGATGAGGCGCAGTT
TGCCGAGCTTGAAACCTTAGGCGAGCTGGTCAAAATCGCCTGGGAATACGATGTGCAGACCATTATCGAAGGGCCAGGGC
ATATCCCGATGCAGCTTATCAAGGAGAATATGGATAAGCAGCTTATCCACTGCGCCGAGGCGCCATTTTATACCTTAGGT
CCACAAATTACCGATATTGCACCGGGTTACGATCATTTCACCTCGGGGATAGGTGCGGCGATGATTGCATGGTATGGCTG
CGCCATGCTCTGCTATGTGACGCCGAAAGAACACTTAGGTTTACCCAATAAGCAGGACGTCAAACAAGGGCTGATTGCCT
ACAAGATTGCCGCCCATGCGGCCGATATTGCTAAAGGTCACCCGGGCGCGCAGATCCGCGACAATGCGTTATCTAAGGCG
CGCTTTGAATTCCGTTGGGAAGACCAATATAACTTAGGGCTTGACCCTGATACCGCGCGTGCCTATCACGATGAATCCCT
GCCACAGGAATCGGCCAAGGTTGCGCATTTTTGCTCTATGTGTGGCCCTAAGTTTTGCTCGATGAAGATCACCCAAGATG
TACGTGACTATGCGGCAAGTTTAGCGGCGGGTGCGTTAGCAGGGGCTTCGTCAAGCCAGAATAATGAAACGACTGAAGCG
CTTGCATCGATAAACTCTGCTCCACAAGGGCAAGCTAGCAAAGAAGCAATCCAGCAGATCAGAGTCAAAACCCAAGATGA
ATTAGCGGCGGCCATGGCGCAAAAATCGGCCGAGTTTGCGGCCTCGGGCGCCAAACTGTATCTGCCGCTAGGTGATGCTA
ATGCAGCCAATACTGCCAACGAATCGGCAAAGCATCAGGATGGGACAGAGCTCCAACCCACAGTACAGGTAGCGGAGGTT
TGA

Upstream 100 bases:

>100_bases
ATTGAGTCCGCAAATCCGCTTAGGGTTTGCAAGGCTCAGCTTCAACACTCAAGTCTCCAGACAAGCAACCCCATTTTTAT
TTGTTAAAGTGAGATTGCTT

Downstream 100 bases:

>100_bases
TAATGACCTCTGCTAAACATGTTAGCCACTTGGCGCCTAAACCTATCGTCTGGACCATTGCGGGTTCAGACAGCGGTGGC
GGCGCGGGGATCCAAGCCGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSSLESSSESIAVNTLGQTSRRAARAQAQAFIDTLKPLQHPNSQKLYLQGSREDLRVGMRQILQTDTRIGGTAAEPTVEK
NPPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDTEVLDCATSDFTQQRLKDDGLDHLRFEAGSSAIVRPRRALP
GKRVSQLHYARQGIITPEMEYVAIRENMALAEVQDEILNRRGQGESFGTVIGKSITPEFVRDEIARGRAIIPLNINHPEA
EPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKV
NGVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELC
VAYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLG
PQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHAADIAKGHPGAQIRDNALSKA
RFEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLAAGALAGASSSQNNETTEA
LASINSAPQGQASKEAIQQIRVKTQDELAAAMAQKSAEFAASGAKLYLPLGDANAANTANESAKHQDGTELQPTVQVAEV

Sequences:

>Translated_720_residues
MSSLESSSESIAVNTLGQTSRRAARAQAQAFIDTLKPLQHPNSQKLYLQGSREDLRVGMRQILQTDTRIGGTAAEPTVEK
NPPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDTEVLDCATSDFTQQRLKDDGLDHLRFEAGSSAIVRPRRALP
GKRVSQLHYARQGIITPEMEYVAIRENMALAEVQDEILNRRGQGESFGTVIGKSITPEFVRDEIARGRAIIPLNINHPEA
EPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKV
NGVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELC
VAYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLG
PQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHAADIAKGHPGAQIRDNALSKA
RFEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLAAGALAGASSSQNNETTEA
LASINSAPQGQASKEAIQQIRVKTQDELAAAMAQKSAEFAASGAKLYLPLGDANAANTANESAKHQDGTELQPTVQVAEV
>Mature_719_residues
SSLESSSESIAVNTLGQTSRRAARAQAQAFIDTLKPLQHPNSQKLYLQGSREDLRVGMRQILQTDTRIGGTAAEPTVEKN
PPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDTEVLDCATSDFTQQRLKDDGLDHLRFEAGSSAIVRPRRALPG
KRVSQLHYARQGIITPEMEYVAIRENMALAEVQDEILNRRGQGESFGTVIGKSITPEFVRDEIARGRAIIPLNINHPEAE
PMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWIIRNSPVPIGTVPIYQALEKVN
GVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELCV
AYDVSLSLGDGMRPGSIADANDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLGP
QITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHAADIAKGHPGAQIRDNALSKAR
FEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSMCGPKFCSMKITQDVRDYAASLAAGALAGASSSQNNETTEAL
ASINSAPQGQASKEAIQQIRVKTQDELAAAMAQKSAEFAASGAKLYLPLGDANAANTANESAKHQDGTELQPTVQVAEV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=603, Percent_Identity=76.9485903814262, Blast_Score=998, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 79094; Mature: 78963

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLESSSESIAVNTLGQTSRRAARAQAQAFIDTLKPLQHPNSQKLYLQGSREDLRVGMR
CCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
QILQTDTRIGGTAAEPTVEKNPPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDT
HHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHEEHHHHHHHHCCCEEEECCCC
EVLDCATSDFTQQRLKDDGLDHLRFEAGSSAIVRPRRALPGKRVSQLHYARQGIITPEME
CHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCC
YVAIRENMALAEVQDEILNRRGQGESFGTVIGKSITPEFVRDEIARGRAIIPLNINHPEA
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
EPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWI
CCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
IRNSPVPIGTVPIYQALEKVNGVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
TAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELCVAYDVSLSLGDGMRPGSIAD
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
ANDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLG
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEECC
PQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHH
ADIAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSM
HHHHCCCCCCCHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHH
CGPKFCSMKITQDVRDYAASLAAGALAGASSSQNNETTEALASINSAPQGQASKEAIQQI
CCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RVKTQDELAAAMAQKSAEFAASGAKLYLPLGDANAANTANESAKHQDGTELQPTVQVAEV
HCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SSLESSSESIAVNTLGQTSRRAARAQAQAFIDTLKPLQHPNSQKLYLQGSREDLRVGMR
CCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
QILQTDTRIGGTAAEPTVEKNPPIPVYDCAGPYSDPAAQINVRQGLAKLRLPWIIERQDT
HHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHEEHHHHHHHHCCCEEEECCCC
EVLDCATSDFTQQRLKDDGLDHLRFEAGSSAIVRPRRALPGKRVSQLHYARQGIITPEME
CHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCC
YVAIRENMALAEVQDEILNRRGQGESFGTVIGKSITPEFVRDEIARGRAIIPLNINHPEA
EEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCC
EPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYIHETREWI
CCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHH
IRNSPVPIGTVPIYQALEKVNGVAEHLTWEVFRDTLIEQAEQGVDYFTIHAGVLLRYVPM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
TAKRVTGIVSRGGSIMAKWCLSHHQENFLYTHFREICELCVAYDVSLSLGDGMRPGSIAD
HHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCC
ANDEAQFAELETLGELVKIAWEYDVQTIIEGPGHIPMQLIKENMDKQLIHCAEAPFYTLG
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEECC
PQITDIAPGYDHFTSGIGAAMIAWYGCAMLCYVTPKEHLGLPNKQDVKQGLIAYKIAAHA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHCCCCCHHHHHHHHHHHHHHHHH
ADIAKGHPGAQIRDNALSKARFEFRWEDQYNLGLDPDTARAYHDESLPQESAKVAHFCSM
HHHHCCCCCCCHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHH
CGPKFCSMKITQDVRDYAASLAAGALAGASSSQNNETTEALASINSAPQGQASKEAIQQI
CCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
RVKTQDELAAAMAQKSAEFAASGAKLYLPLGDANAANTANESAKHQDGTELQPTVQVAEV
HCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]