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The map label for this gene is hisH [H]

Identifier: 117920295

GI number: 117920295

Start: 2191578

End: 2192228

Strand: Reverse

Name: hisH [H]

Synonym: Shewana3_1850

Alternate gene names: 117920295

Gene position: 2192228-2191578 (Counterclockwise)

Preceding gene: 117920296

Following gene: 117920294

Centisome position: 44.09

GC content: 51.31

Gene sequence:

>651_bases
ATGTCAGCAAACATAGATGAATTTGATGTTGTCATCATAGACACAGGTTGCGCCAACTTAAGCTCGGTCCGTTTCGCCTT
TGAGCGCCTCGGCGCTAAGGTGTTAGTCACCGATGACAAGGCCAGCATTAAAGCCGCCAAACGTGTGGTACTGCCAGGTG
TCGGCAGCGCTGGCGCCGCGATGGCTTCGTTAACGGAAAAAGCCTTAGTCGAATTAATCCAAGGGTTAACTCAACCCGTG
CTGGGCGTCTGTTTAGGGATGCAAATGCTCACCCTGCTCTCTAAAGAACGCGGCGGCCAAGCGTTAGATTGCCAATGCTT
AGGCATTATTCCAACCGAGATTGACGAGCTAGACCGTCAAATACTGAAGGCCGAAGGTTTACCTCTGCCGCACATGGGCT
GGAACCAACTGACCTTTAGCAATCCGTCTCAGGTTCACCCGCTGTTTACCGGTGTTCCAGCTGGCAGTTATGTGTATTTT
GTGCACAGCTACCGCGCGCCATTAAGCGACTACACCTTGGCACAATGCCGTTATGGTGAAGACTTTAGCGCCGCCATCGG
TAAAGACAACTTTATGGGCGTGCAATTTCACCCCGAAAAGAGCGCCGCCGTGGGTGCGCAAATCCTAGGTAACTTTTTAA
AAATGCAGTAA

Upstream 100 bases:

>100_bases
TGATTAGCGAATAATACAAAGCCTGATGATTTTAACGAGGGCAGTTCCGGAATGCGGCGACAAGCCACTTCCCCCACTGA
CCCATCAATAGGAAAACCCA

Downstream 100 bases:

>100_bases
ATGCACCAAAAAGAATTTAAGGACAACAGATGATCATTCCAGCAATTGATTTAATTGATGGCCAGATAGTTAGGCTCTAC
CAAGGGGATTATGGGCAGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSANIDEFDVVIIDTGCANLSSVRFAFERLGAKVLVTDDKASIKAAKRVVLPGVGSAGAAMASLTEKALVELIQGLTQPV
LGVCLGMQMLTLLSKERGGQALDCQCLGIIPTEIDELDRQILKAEGLPLPHMGWNQLTFSNPSQVHPLFTGVPAGSYVYF
VHSYRAPLSDYTLAQCRYGEDFSAAIGKDNFMGVQFHPEKSAAVGAQILGNFLKMQ

Sequences:

>Translated_216_residues
MSANIDEFDVVIIDTGCANLSSVRFAFERLGAKVLVTDDKASIKAAKRVVLPGVGSAGAAMASLTEKALVELIQGLTQPV
LGVCLGMQMLTLLSKERGGQALDCQCLGIIPTEIDELDRQILKAEGLPLPHMGWNQLTFSNPSQVHPLFTGVPAGSYVYF
VHSYRAPLSDYTLAQCRYGEDFSAAIGKDNFMGVQFHPEKSAAVGAQILGNFLKMQ
>Mature_215_residues
SANIDEFDVVIIDTGCANLSSVRFAFERLGAKVLVTDDKASIKAAKRVVLPGVGSAGAAMASLTEKALVELIQGLTQPVL
GVCLGMQMLTLLSKERGGQALDCQCLGIIPTEIDELDRQILKAEGLPLPHMGWNQLTFSNPSQVHPLFTGVPAGSYVYFV
HSYRAPLSDYTLAQCRYGEDFSAAIGKDNFMGVQFHPEKSAAVGAQILGNFLKMQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=50.2415458937198, Blast_Score=201, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319725, Length=224, Percent_Identity=34.375, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23193; Mature: 23062

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANIDEFDVVIIDTGCANLSSVRFAFERLGAKVLVTDDKASIKAAKRVVLPGVGSAGAA
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHEEECCCCCCHHHH
MASLTEKALVELIQGLTQPVLGVCLGMQMLTLLSKERGGQALDCQCLGIIPTEIDELDRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCHHHHHHHHH
ILKAEGLPLPHMGWNQLTFSNPSQVHPLFTGVPAGSYVYFVHSYRAPLSDYTLAQCRYGE
HHHHCCCCCCCCCCCCEECCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCHHHHHCCCC
DFSAAIGKDNFMGVQFHPEKSAAVGAQILGNFLKMQ
CHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SANIDEFDVVIIDTGCANLSSVRFAFERLGAKVLVTDDKASIKAAKRVVLPGVGSAGAA
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHEEECCCCCCHHHH
MASLTEKALVELIQGLTQPVLGVCLGMQMLTLLSKERGGQALDCQCLGIIPTEIDELDRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCHHHHHHHHH
ILKAEGLPLPHMGWNQLTFSNPSQVHPLFTGVPAGSYVYFVHSYRAPLSDYTLAQCRYGE
HHHHCCCCCCCCCCCCEECCCCCCCCCCEECCCCCCEEEEEEECCCCCCCCHHHHHCCCC
DFSAAIGKDNFMGVQFHPEKSAAVGAQILGNFLKMQ
CHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]