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The map label for this gene is rlmN [H]

Identifier: 117919675

GI number: 117919675

Start: 1429449

End: 1430570

Strand: Direct

Name: rlmN [H]

Synonym: Shewana3_1226

Alternate gene names: 117919675

Gene position: 1429449-1430570 (Clockwise)

Preceding gene: 117919672

Following gene: 117919676

Centisome position: 28.75

GC content: 49.2

Gene sequence:

>1122_bases
ATGAGTGAAAAAAAGATCAATTTATTAGACCTTGATCGTAAGGCGATGCGCGCATTATTTGCGGATCTGGGTGAGAAGCC
TTTCCGTGCCGATCAGCTGATGAAATGGATCTATCATTTCGGTGTCAGTGACTTCGAAGAGATGACTAACATCAACAAAG
TGTTACGCCAAAAACTGGCGGCGCGCTGTGAGATTGTGGCGCCTGAGATTTCTAGCTATCAAAAATCCACCGATGGCACA
ATTAAGTTCGCCATCCATGTCGGCGAAGGCCAAGAAGTTGAAACCGTATATATTCCTGAAGATGACCGCGCCACCCTGTG
TGTGTCGTCTCAAGTGGGCTGCGCCCTCGAATGTACTTTCTGCTCGACCGCACAGCAAGGCTTTAACCGCAACTTAACTG
TCTCTGAAATTGTCGGTCAAATTTGGCGTGTTTCCCACTTCTTAGGGTTCGCCAAAGAAACCGGCGAGCGCCCAATCACC
AACGTGGTGATGATGGGCATGGGTGAACCGCTGCTTAACCTTGCCAACGTGATCCCTGCGATGGATATCATGCTGGACGA
CTTCGGCTTTAGCCTGTCAAAACGCCGTGTGACCCTGTCAACTTCGGGCGTAGTGCCAGCATTAGATAAACTCGGTGATG
CCCTCGATGTGGCGCTGGCCGTGAGTATCCATGCGCCAAACGATGAACTGCGCGATATTCTGGTTCCCGTAAATAAAAAG
TATCCGCTGCAGGAATTCCTCGCGGGAATTCGCCGTTATATCGCTAAATCTAACGCTAACCGTGGCCGCGTGACTGTGGA
GTATGTGATGCTCGATCACATCAACGACAGTACTGAACAAGCCCATGAATTAGCACAATTAATGAAGGATACGCCCTGCA
AGGTTAACCTGATCCCGTTCAACCCATACCCAGGCTCGCCCTATGGTCGCTCGTCAAACTCGCGCATCGACCGTTTTTCT
AAGGTATTGATGGAATATGGCTTAACCGTGATTGTACGCAAAACCCGTGGTGATGATATTGACGCGGCCTGTGGACAGTT
AGCGGGTGATATTCGCGATCGAACCAAGCGTTTAGCAAAAAAACGCATGCAAGAAAATCAGATTTCGGTCACAATGAATT
AA

Upstream 100 bases:

>100_bases
CGCCGATTCTAGCATAGGAGTGTGGAGTTGCTGGCTAAAATTTTTCTGCGTCTGTATAATTCGCCGCCCTGTTGCAGGTT
TTGGTGTTGAGGTTAGTCCT

Downstream 100 bases:

>100_bases
CTCTTTGTTTGCACAGATTTATAGGCTTGCCCATGAAGCATGGATTGTCAGGGTTATCATTAGCAATTGCCCTGTCATTA
TTAGTGACAGGATGCGTAAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSYQKSTDGT
IKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKETGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKK
YPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAQLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Sequences:

>Translated_373_residues
MSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSYQKSTDGT
IKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKETGERPIT
NVVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKK
YPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAQLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFS
KVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN
>Mature_372_residues
SEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLAARCEIVAPEISSYQKSTDGTI
KFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTFCSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKETGERPITN
VVMMGMGEPLLNLANVIPAMDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKKY
PLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAQLMKDTPCKVNLIPFNPYPGSPYGRSSNSRIDRFSK
VLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAKKRMQENQISVTMN

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=75.6830601092896, Blast_Score=598, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41713; Mature: 41581

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLA
CCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARCEIVAPEISSYQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKETGERPITNVVMMGMGEPLLNLANVIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCHHHEEEEEEEEECCCHHHHHHCCCCCCC
YPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAQLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC
>Mature Secondary Structure 
SEKKINLLDLDRKAMRALFADLGEKPFRADQLMKWIYHFGVSDFEEMTNINKVLRQKLA
CCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ARCEIVAPEISSYQKSTDGTIKFAIHVGEGQEVETVYIPEDDRATLCVSSQVGCALECTF
HHHEEECCCHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCEEEEEE
CSTAQQGFNRNLTVSEIVGQIWRVSHFLGFAKETGERPITNVVMMGMGEPLLNLANVIPA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHH
MDIMLDDFGFSLSKRRVTLSTSGVVPALDKLGDALDVALAVSIHAPNDELRDILVPVNKK
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHCCHHHEEEEEEEEECCCHHHHHHCCCCCCC
YPLQEFLAGIRRYIAKSNANRGRVTVEYVMLDHINDSTEQAHELAQLMKDTPCKVNLIPF
CCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEC
NPYPGSPYGRSSNSRIDRFSKVLMEYGLTVIVRKTRGDDIDAACGQLAGDIRDRTKRLAK
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
KRMQENQISVTMN
HHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA