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The map label for this gene is trpI [H]

Identifier: 117918984

GI number: 117918984

Start: 624333

End: 625199

Strand: Reverse

Name: trpI [H]

Synonym: Shewana3_0530

Alternate gene names: 117918984

Gene position: 625199-624333 (Counterclockwise)

Preceding gene: 117918993

Following gene: 117918982

Centisome position: 12.57

GC content: 48.67

Gene sequence:

>867_bases
ATGAGACACTTAAAAGCATTCTATGTATTTCACATTACGGCCGAGTCCTCGAGTTACAGTCAGGCCGCAGAGAAATTGCA
TATCACTCACGGTGCGGTCAGTAAGCAAATAAAACTCTTGGAAAACCACTTATCGCAAATACTGTTTTATAAGCAAGGCC
GAGGGATGTGCCTCACTCAAGAGGGGGAATTGCTTAAGCGCTACACAGATATTGCCTTTAACACCTTAGATAGTGGGGTA
AAGCAGCTGACTCGAGAGGGCCATCATTACCTTGAGGTTTCCTGTGAACCCACACTCACCATGCGCTGGTTAATGCCGCG
GTTAGCCAGTTTTTTCCAGCTAACGGGTATAGACGTTCGATTATCAACGGCTGGCGGGCCAGTCAAACTGGCGGGATCAG
GATTATCTTTGGCCATTCGGCGAGACGATTTTCATATCCCCCCTGAATACAGCAAGCACAATTTAGTTAATGAGTGGGTT
GGCCCAGTGTTTTCGCCTGAATATTGGCGTCAAATTCAGCAAAACTGGGGCGATATTGTCCTGCTGCATAGTGACTCCCG
GCCGTCGGCATGGTCGGATTGGTCGCAGCGTTCGGGACACAATTTCTCAGCCAATAAGTCAAAATCCTTCGAGCATTTCT
ATTTCTGCCTGCAGGCGGCGGTTGATGGACTCGGAGGCGCCATAGGCTCGTATCCCCTGATTGTTGACGATCTAACAAGC
GGCAGGCTCGTCGCGCCATTTGGCTTTATCCCATCAGGGCATAATTATGTGTTGTTGAGTCATACGGAAAAGCTTGGCAA
CAATGAATTAGCCTTCTTCAACTGGTTAGAAGAGACCCTCTCCTTATGCCAACCCGAAACAGCCTAG

Upstream 100 bases:

>100_bases
TTTCCGCTCCTCAGGGTTCATTAGCAAGCGGTAATTGTATCGAGTCGCTGGTGAGTTATAATCGAAAAGAAGGCACCAAA
ACAGTGAGAAAAAGTCACAT

Downstream 100 bases:

>100_bases
GCCACGAGGCTCGTATAAAATGAACTTACCGCTCCGAGTGGGAAGCCTAACCAATAGCTGCATCTCGATTGATGAGCTAA
ACACCTTTTTAGTCAGATCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRHLKAFYVFHITAESSSYSQAAEKLHITHGAVSKQIKLLENHLSQILFYKQGRGMCLTQEGELLKRYTDIAFNTLDSGV
KQLTREGHHYLEVSCEPTLTMRWLMPRLASFFQLTGIDVRLSTAGGPVKLAGSGLSLAIRRDDFHIPPEYSKHNLVNEWV
GPVFSPEYWRQIQQNWGDIVLLHSDSRPSAWSDWSQRSGHNFSANKSKSFEHFYFCLQAAVDGLGGAIGSYPLIVDDLTS
GRLVAPFGFIPSGHNYVLLSHTEKLGNNELAFFNWLEETLSLCQPETA

Sequences:

>Translated_288_residues
MRHLKAFYVFHITAESSSYSQAAEKLHITHGAVSKQIKLLENHLSQILFYKQGRGMCLTQEGELLKRYTDIAFNTLDSGV
KQLTREGHHYLEVSCEPTLTMRWLMPRLASFFQLTGIDVRLSTAGGPVKLAGSGLSLAIRRDDFHIPPEYSKHNLVNEWV
GPVFSPEYWRQIQQNWGDIVLLHSDSRPSAWSDWSQRSGHNFSANKSKSFEHFYFCLQAAVDGLGGAIGSYPLIVDDLTS
GRLVAPFGFIPSGHNYVLLSHTEKLGNNELAFFNWLEETLSLCQPETA
>Mature_288_residues
MRHLKAFYVFHITAESSSYSQAAEKLHITHGAVSKQIKLLENHLSQILFYKQGRGMCLTQEGELLKRYTDIAFNTLDSGV
KQLTREGHHYLEVSCEPTLTMRWLMPRLASFFQLTGIDVRLSTAGGPVKLAGSGLSLAIRRDDFHIPPEYSKHNLVNEWV
GPVFSPEYWRQIQQNWGDIVLLHSDSRPSAWSDWSQRSGHNFSANKSKSFEHFYFCLQAAVDGLGGAIGSYPLIVDDLTS
GRLVAPFGFIPSGHNYVLLSHTEKLGNNELAFFNWLEETLSLCQPETA

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=31.0104529616725, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786448, Length=247, Percent_Identity=27.9352226720648, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=28.4246575342466, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=34.1880341880342, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32489; Mature: 32489

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLKAFYVFHITAESSSYSQAAEKLHITHGAVSKQIKLLENHLSQILFYKQGRGMCLTQ
CCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
EGELLKRYTDIAFNTLDSGVKQLTREGHHYLEVSCEPTLTMRWLMPRLASFFQLTGIDVR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEE
LSTAGGPVKLAGSGLSLAIRRDDFHIPPEYSKHNLVNEWVGPVFSPEYWRQIQQNWGDIV
EECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEE
LLHSDSRPSAWSDWSQRSGHNFSANKSKSFEHFYFCLQAAVDGLGGAIGSYPLIVDDLTS
EEECCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GRLVAPFGFIPSGHNYVLLSHTEKLGNNELAFFNWLEETLSLCQPETA
CCEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRHLKAFYVFHITAESSSYSQAAEKLHITHGAVSKQIKLLENHLSQILFYKQGRGMCLTQ
CCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
EGELLKRYTDIAFNTLDSGVKQLTREGHHYLEVSCEPTLTMRWLMPRLASFFQLTGIDVR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEE
LSTAGGPVKLAGSGLSLAIRRDDFHIPPEYSKHNLVNEWVGPVFSPEYWRQIQQNWGDIV
EECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEE
LLHSDSRPSAWSDWSQRSGHNFSANKSKSFEHFYFCLQAAVDGLGGAIGSYPLIVDDLTS
EEECCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GRLVAPFGFIPSGHNYVLLSHTEKLGNNELAFFNWLEETLSLCQPETA
CCEEECCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]