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The map label for this gene is pleD [H]

Identifier: 117918906

GI number: 117918906

Start: 524652

End: 525695

Strand: Reverse

Name: pleD [H]

Synonym: Shewana3_0449

Alternate gene names: 117918906

Gene position: 525695-524652 (Counterclockwise)

Preceding gene: 117918908

Following gene: 117918905

Centisome position: 10.57

GC content: 48.08

Gene sequence:

>1044_bases
ATGGAAAGAAGATTCAACCCTTTAGACTTATCTGATAACGCTCAAGGACATCAGATCTGCCTTCCTGTCGAGTTTGTGCA
AGCGCTCGCAGTGGCGAATTCACTTCAAGAAGTACTCGATACCGTTTCCCAATGGCTCTACCAACTGTTTGATGCTGAAA
GGGCCAGCATTACCTTGAGGGACGACGCCGACTTTCTCAAAATCTATTCCATCCGGGGTAACAAAGCGATCCCGCTCGAT
TTCAGGGTACCTATCGCCAATACCCTTGTGGGTCGCGCCTTCACGGAAAAGACGGTACTCATCTGCGATGAATTAGATAA
ATCCACCGAACTGGACTGCATCATGCTGGCGCAACACGGCATGCAAACCTGTATGGATGCACCGCTGCTTTATGGCGATG
TCTGCCTAGGCACGCTCAATGTCGCCCATAAGAAATCATCTTATTACAGTGAGTTACATGCCATAGAGTTGCAATGCTTA
GCCAATTGGATTGGGCTTAATATTAGCCTGCGACTACAACTGAAAGAACTCGAATACCTTGCCTCAACGGATCATTTAAC
AGGCGCGGCAAATCGACGGGTGTTTACCCAAAAAATTCGCCAAGCCATGACTGAGTTCCATGAAAATGGCCAGTCGTTTT
TCTTTGGCTTGATGGATATTGACCATTTTAAGATGTTGAATGACCAATTTGGTCATAGTGCAGGCGACTATGTGTTGCAG
CGTATCGTCGCCATTTCCGAAACTATGATGACCAGTCCGGGGAAATTCTTTCGCATTGGTGGTGAGGAGTTTGCGATTAT
CTTCCCCGAGCAAAGCGAGTATGAGCCCTTGCGGTTTTTTAACGATATCAGGGAAGCGATCGCCCTTGCCGCGATGGATT
ACGATGGCATACCAATCAGTGTTAGCGTGAGCATTGGGTTTACCGCAATGCACATTCATGACAACACCCCAGAATCCTTG
CTGAGGCGCTCGGATAAGGCGCTCTACCAAGCCAAACTCGCTGGCAGAAATCGCGTGCTCATGTTTAACAGTGCGCAGGC
ATAA

Upstream 100 bases:

>100_bases
GATTGCAGAGGTTGCCAACTCCACTTATATTTCAGCTAGAACCTCAATTGGGTATTTAAAATATCGCTTTAGATAGCCAA
AGGCTTTAAGGGGCATAAGT

Downstream 100 bases:

>100_bases
GGGTAAATAACAGGGACGTTATAAATCCTTTGCAGAAAAAATGCACAATAAAACTGCAAGTTGGCAACATTTAAAACTCA
TTATCTCTATGAATAATAAA

Product: diguanylate cyclase with GAF sensor

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MERRFNPLDLSDNAQGHQICLPVEFVQALAVANSLQEVLDTVSQWLYQLFDAERASITLRDDADFLKIYSIRGNKAIPLD
FRVPIANTLVGRAFTEKTVLICDELDKSTELDCIMLAQHGMQTCMDAPLLYGDVCLGTLNVAHKKSSYYSELHAIELQCL
ANWIGLNISLRLQLKELEYLASTDHLTGAANRRVFTQKIRQAMTEFHENGQSFFFGLMDIDHFKMLNDQFGHSAGDYVLQ
RIVAISETMMTSPGKFFRIGGEEFAIIFPEQSEYEPLRFFNDIREAIALAAMDYDGIPISVSVSIGFTAMHIHDNTPESL
LRRSDKALYQAKLAGRNRVLMFNSAQA

Sequences:

>Translated_347_residues
MERRFNPLDLSDNAQGHQICLPVEFVQALAVANSLQEVLDTVSQWLYQLFDAERASITLRDDADFLKIYSIRGNKAIPLD
FRVPIANTLVGRAFTEKTVLICDELDKSTELDCIMLAQHGMQTCMDAPLLYGDVCLGTLNVAHKKSSYYSELHAIELQCL
ANWIGLNISLRLQLKELEYLASTDHLTGAANRRVFTQKIRQAMTEFHENGQSFFFGLMDIDHFKMLNDQFGHSAGDYVLQ
RIVAISETMMTSPGKFFRIGGEEFAIIFPEQSEYEPLRFFNDIREAIALAAMDYDGIPISVSVSIGFTAMHIHDNTPESL
LRRSDKALYQAKLAGRNRVLMFNSAQA
>Mature_347_residues
MERRFNPLDLSDNAQGHQICLPVEFVQALAVANSLQEVLDTVSQWLYQLFDAERASITLRDDADFLKIYSIRGNKAIPLD
FRVPIANTLVGRAFTEKTVLICDELDKSTELDCIMLAQHGMQTCMDAPLLYGDVCLGTLNVAHKKSSYYSELHAIELQCL
ANWIGLNISLRLQLKELEYLASTDHLTGAANRRVFTQKIRQAMTEFHENGQSFFFGLMDIDHFKMLNDQFGHSAGDYVLQ
RIVAISETMMTSPGKFFRIGGEEFAIIFPEQSEYEPLRFFNDIREAIALAAMDYDGIPISVSVSIGFTAMHIHDNTPESL
LRRSDKALYQAKLAGRNRVLMFNSAQA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=36.144578313253, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=32.0441988950276, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=33.1288343558282, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=30.5882352941176, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87081977, Length=323, Percent_Identity=27.5541795665635, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786584, Length=188, Percent_Identity=28.7234042553192, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=33.7278106508876, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=28.7425149700599, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=32.5153374233129, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=28.2352941176471, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=28.4883720930233, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=32.4022346368715, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39194; Mature: 39194

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERRFNPLDLSDNAQGHQICLPVEFVQALAVANSLQEVLDTVSQWLYQLFDAERASITLR
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DDADFLKIYSIRGNKAIPLDFRVPIANTLVGRAFTEKTVLICDELDKSTELDCIMLAQHG
CCCCEEEEEEECCCEEECEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
MQTCMDAPLLYGDVCLGTLNVAHKKSSYYSELHAIELQCLANWIGLNISLRLQLKELEYL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
ASTDHLTGAANRRVFTQKIRQAMTEFHENGQSFFFGLMDIDHFKMLNDQFGHSAGDYVLQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHH
RIVAISETMMTSPGKFFRIGGEEFAIIFPEQSEYEPLRFFNDIREAIALAAMDYDGIPIS
HHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
VSVSIGFTAMHIHDNTPESLLRRSDKALYQAKLAGRNRVLMFNSAQA
EEEEECEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MERRFNPLDLSDNAQGHQICLPVEFVQALAVANSLQEVLDTVSQWLYQLFDAERASITLR
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DDADFLKIYSIRGNKAIPLDFRVPIANTLVGRAFTEKTVLICDELDKSTELDCIMLAQHG
CCCCEEEEEEECCCEEECEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
MQTCMDAPLLYGDVCLGTLNVAHKKSSYYSELHAIELQCLANWIGLNISLRLQLKELEYL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
ASTDHLTGAANRRVFTQKIRQAMTEFHENGQSFFFGLMDIDHFKMLNDQFGHSAGDYVLQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHH
RIVAISETMMTSPGKFFRIGGEEFAIIFPEQSEYEPLRFFNDIREAIALAAMDYDGIPIS
HHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
VSVSIGFTAMHIHDNTPESLLRRSDKALYQAKLAGRNRVLMFNSAQA
EEEEECEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA