Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 117918865

GI number: 117918865

Start: 467291

End: 468556

Strand: Reverse

Name: rho [H]

Synonym: Shewana3_0408

Alternate gene names: 117918865

Gene position: 468556-467291 (Counterclockwise)

Preceding gene: 117918866

Following gene: 117918863

Centisome position: 9.42

GC content: 46.6

Gene sequence:

>1266_bases
ATGAACTTAACTGAATTAAAAGACACGCCGATATCCGACCTAGTCTCGCTAGCCGAAAATATGAAACTCGAAAATATGGC
CCGCGCTCGCAAGCAGGACATTATTTTCTCTATTCTTAAAGCCCACGCCAAAAGCGGCGAAGACATTTTCGGTGGCGGTG
TATTAGAGATACTCCAAGACGGATTCGGTTTCTTACGTAGTGCAGATGGATCTTATTTAGCTGGCCCGGATGATATTTAT
GTATCACCAAGCCAAATTCGCCGCTTTAACATGCGAACGGGCGACACGATTTTTGGCAAGATTCGCCCACCAAAAGAAGG
TGAACGTTATTTTGCCCTGCTAAAAGTTAACGAAGTTAACTTCGATAAACCTGAAAACTCTCGCAACAAAATCCTCTTTG
AAAACTTAACCCCACTCCACGCAGAAGAACGTATGCGCATGGAGCGTGGTAATGGCTCTACTGAAGACATCACTGCCCGT
ATTCTGGATTTATGCTCACCTATCGGTAAGGGTCAACGTGGTCTGATCGTCGCACCACCAAAAGCGGGTAAAACCTTATT
ACTGCAAAACATTGCTCAGTCAATCACCTACAACAACCCAGAAGTTGTGTTGATGGTACTGCTGATCGATGAACGTCCTG
AAGAAGTGACCGAGATGCAACGCCTAGTTCAAGGTGAAGTGATTGCATCGACCTTCGACGAACCAGCTAGCCGTCACGTA
CAAGTGGCTGAAATGGTAATTGAAAAAGCCAAACGTTTAGTTGAGCACAAGAAAGACGTTGTGATCTTACTCGACTCTAT
CACTCGTTTGGCTCGCGCCTACAACACTGTTATCCCATCATCGGGCAAAGTGTTAACTGGTGGTGTGGATGCAAACGCCC
TGCACCGTCCTAAGCGCTTCTTCGGCGCGGCTCGTAACATCGAAAACGGCGGCAGCTTAACGATTATTGCTACCGCACTG
GTTGATACCGGCTCGAAAATGGACGAAGTGATCTACGAAGAGTTCAAAGGTACAGGTAACCAAGAACTGCATCTGTCACG
CAAGGCCGCTGAAAAACGCGTATTCCCAGCGATTGACTTCAACCGCAGTGGTACTCGTCGCGAAGAAAAACTGACCACAC
CTGATGAACTGCAAAAGATGTGGATCCTGCGTAAGATCTTAAATCCTATGGATGAAGTCAGCGCAATGGAATTCCTGATC
GATAAACTGGCTATGACTAAGACCAACGAAGAATTCTTCACCGCGATGAAACGCGCTAAGTCTTAA

Upstream 100 bases:

>100_bases
TTCTAACTAAACCACTTCTCGCTCGTCAATCACTATCCTGAATCACAAACTTTACTGATTGATTGCGGTAAGCTTTGTCG
CGTAACACAAGATCCCCAAC

Downstream 100 bases:

>100_bases
TCCTAGGTTTAGGGTCAAATCTTTCAAAAAAGCCGCTTAATGCGGCTTTTTTATTGCGCCGAGTTTGGCAGTGCAAGATT
AAATCACAGAGCGTTGAGCC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNLTELKDTPISDLVSLAENMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSADGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVNEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITAR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQNIAQSITYNNPEVVLMVLLIDERPEEVTEMQRLVQGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIENGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVSAMEFLI
DKLAMTKTNEEFFTAMKRAKS

Sequences:

>Translated_421_residues
MNLTELKDTPISDLVSLAENMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSADGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVNEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITAR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQNIAQSITYNNPEVVLMVLLIDERPEEVTEMQRLVQGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIENGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVSAMEFLI
DKLAMTKTNEEFFTAMKRAKS
>Mature_421_residues
MNLTELKDTPISDLVSLAENMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQDGFGFLRSADGSYLAGPDDIY
VSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVNEVNFDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITAR
ILDLCSPIGKGQRGLIVAPPKAGKTLLLQNIAQSITYNNPEVVLMVLLIDERPEEVTEMQRLVQGEVIASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRFFGAARNIENGGSLTIIATAL
VDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDFNRSGTRREEKLTTPDELQKMWILRKILNPMDEVSAMEFLI
DKLAMTKTNEEFFTAMKRAKS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=87.1121718377088, Blast_Score=766, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47163; Mature: 47163

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTELKDTPISDLVSLAENMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQD
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADGSYLAGPDDIYVSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVNEVN
HHHHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEECCC
FDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITARILDLCSPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
KAGKTLLLQNIAQSITYNNPEVVLMVLLIDERPEEVTEMQRLVQGEVIASTFDEPASRHV
CCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHH
FGAARNIENGGSLTIIATALVDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDF
HHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCC
NRSGTRREEKLTTPDELQKMWILRKILNPMDEVSAMEFLIDKLAMTKTNEEFFTAMKRAK
CCCCCCCHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
S
C
>Mature Secondary Structure
MNLTELKDTPISDLVSLAENMKLENMARARKQDIIFSILKAHAKSGEDIFGGGVLEILQD
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADGSYLAGPDDIYVSPSQIRRFNMRTGDTIFGKIRPPKEGERYFALLKVNEVN
HHHHHCCCCCCCCCCCCCEEECHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEECCC
FDKPENSRNKILFENLTPLHAEERMRMERGNGSTEDITARILDLCSPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
KAGKTLLLQNIAQSITYNNPEVVLMVLLIDERPEEVTEMQRLVQGEVIASTFDEPASRHV
CCCHHHHHHHHHHHHCCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDANALHRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHH
FGAARNIENGGSLTIIATALVDTGSKMDEVIYEEFKGTGNQELHLSRKAAEKRVFPAIDF
HHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCC
NRSGTRREEKLTTPDELQKMWILRKILNPMDEVSAMEFLIDKLAMTKTNEEFFTAMKRAK
CCCCCCCHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]