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The map label for this gene is glnG [H]

Identifier: 117918715

GI number: 117918715

Start: 289031

End: 290440

Strand: Reverse

Name: glnG [H]

Synonym: Shewana3_0258

Alternate gene names: 117918715

Gene position: 290440-289031 (Counterclockwise)

Preceding gene: 117918716

Following gene: 117918710

Centisome position: 5.84

GC content: 53.33

Gene sequence:

>1410_bases
ATGAGTGAACAAGTGTGGATCCTCGACGATGACAGTTCGATACGTTGGGTGCTCGAAAAGGCGCTCCAAGGCGCGAAACT
CAGCACCGCCAGCTTTGCCGCGGCCGAATCACTTTGGCAAGCATTAGAGATTTCCCAGCCACGGGTCATAGTCTCAGATA
TTCGCATGCCGGGCACAGACGGCTTAACCCTGTTAGAAAGGCTGCAAATCCACTACCCGCATATTCCCGTCATCATCATG
ACTGCACATTCGGATTTAGACAGTGCCGTCAGCGCCTATCAGGCGGGGGCATTTGAGTATTTGCCCAAACCCTTCGATAT
TGATGAGGCGATATCCTTAGTCGAGCGCGCCCTAACCCATGCGACCGAGCAAAGCCCCGCACCTGCGGCGCAGGAGACGC
AAGTCAAAACCCCCGAAATCATAGGTGAAGCGCCCGCGATGCAGGAGGTGTTTCGCGCCATTGGCCGGTTATCACGCTCC
TCAATCAGCGTGCTCATCAACGGCCAATCGGGTACGGGTAAAGAGCTAGTCGCAGGTGCATTGCACAAACACAGCCCGCG
CAAAGACAAGCCCTTTATCGCCTTGAATATGGCGGCAATTCCCAAAGATTTAATCGAATCGGAGCTCTTCGGCCATGAAA
AAGGCGCCTTCACCGGCGCGGCCAACGTGCGCCAAGGACGCTTCGAGCAAGCCAATGGCGGCACCCTGTTTTTAGATGAA
ATCGGCGATATGCCGCTGGACGTACAAACCCGTTTGCTGCGGGTACTCGCCGATGGACAATTTTATCGTGTCGGCGGCCA
TAGCGCCGTCCAAGTGGATGTGCGCATTATTGCTGCCACCCACCAAGATCTGGAGCAACTCGTGCTCAAAGGGGGATTTA
GGGAAGACCTATTCCATCGGCTCAATGTTATCCGCGTCCACTTGCCGCCGCTGTCACAACGCCGTGAGGATATTCCCCAA
CTGGCAACCCACTTTCTAGCCTCCGCCGCTAAGGAGATTGGTGTCGAGGCAAAAATCCTCACCAAAGAGACCGCCGCCAA
GCTATCGCAACTGCCATGGCCTGGGAACGTGAGACAACTCGAGAATACCTGTCGCTGGCTCACGGTAATGGCCTCAGGGC
AAGAAATCCTGCCCCAGGATTTACCCCAAGAGCTACTTAAAGAACCCGCCAGTATTAATCCTATGGCGAAAGGCAGCCAA
GATTGGCAATCGGCACTCACAGAGTGGATTGACCAAAAACTGTCTGAGGGAAATAGCGATTTATTAACTGAGGTTCAGCC
GGCCTTTGAACGTATTTTGCTCGAAACCGCATTACGCCATACCCAAGGGCATAAACAAGAAGCGGCAAAACGTTTAGGTT
GGGGAAGAAATACCTTAACGCGAAAACTCAAAGAATTGTCTATGGATTAA

Upstream 100 bases:

>100_bases
TCCAGCCCTGGGCATACCGAATTTATCATTTCGTTACCGATTTTAAGCGCCAAATAATCAAGACAAACCAACACATACCG
TATGAGGAAGCAAAATGCGA

Downstream 100 bases:

>100_bases
GCATCCATTAAAAAGCAAAAAAGGGATATCACTATCCCTTTTTTATTATTTGGATTAACTCTAAATCATTAGAACTGGTA
ATGTACACCAATCGCCAGTT

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTDGLTLLERLQIHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQ
DWQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD

Sequences:

>Translated_469_residues
MSEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTDGLTLLERLQIHYPHIPVIIM
TAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRS
SISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHRLNVIRVHLPPLSQRREDIPQ
LATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQ
DWQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
>Mature_468_residues
SEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTDGLTLLERLQIHYPHIPVIIMT
AHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTHATEQSPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRSS
ISVLINGQSGTGKELVAGALHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDEI
GDMPLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHRLNVIRVHLPPLSQRREDIPQL
ATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQLENTCRWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQD
WQSALTEWIDQKLSEGNSDLLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=465, Percent_Identity=70.5376344086021, Blast_Score=670, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=469, Percent_Identity=40.5117270788913, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=42.1166306695464, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=39.4144144144144, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=40.8284023668639, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=41.1411411411411, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1789233, Length=351, Percent_Identity=42.4501424501425, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1787583, Length=340, Percent_Identity=40.8823529411765, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=256, Percent_Identity=45.3125, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=377, Percent_Identity=40.053050397878, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=329, Percent_Identity=38.6018237082067, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=32.3717948717949, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=38.2222222222222, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789809, Length=132, Percent_Identity=28.7878787878788, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51847; Mature: 51716

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTD
CCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
GLTLLERLQIHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTH
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
ATEQSPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAGA
HHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQDWQSALTEWIDQKLSEGNSD
HHHHHEEHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHH
LLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQVWILDDDSSIRWVLEKALQGAKLSTASFAAAESLWQALEISQPRVIVSDIRMPGTD
CCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
GLTLLERLQIHYPHIPVIIMTAHSDLDSAVSAYQAGAFEYLPKPFDIDEAISLVERALTH
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHH
ATEQSPAPAAQETQVKTPEIIGEAPAMQEVFRAIGRLSRSSISVLINGQSGTGKELVAGA
HHCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHH
LHKHSPRKDKPFIALNMAAIPKDLIESELFGHEKGAFTGAANVRQGRFEQANGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHH
IGDMPLDVQTRLLRVLADGQFYRVGGHSAVQVDVRIIAATHQDLEQLVLKGGFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHCCCCHHHHHHH
LNVIRVHLPPLSQRREDIPQLATHFLASAAKEIGVEAKILTKETAAKLSQLPWPGNVRQL
HCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHH
ENTCRWLTVMASGQEILPQDLPQELLKEPASINPMAKGSQDWQSALTEWIDQKLSEGNSD
HHHHHEEHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHH
LLTEVQPAFERILLETALRHTQGHKQEAAKRLGWGRNTLTRKLKELSMD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]