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The map label for this gene is ntrB [H]

Identifier: 117918716

GI number: 117918716

Start: 290484

End: 291530

Strand: Reverse

Name: ntrB [H]

Synonym: Shewana3_0259

Alternate gene names: 117918716

Gene position: 291530-290484 (Counterclockwise)

Preceding gene: 117918717

Following gene: 117918715

Centisome position: 5.86

GC content: 52.53

Gene sequence:

>1047_bases
ATGGATAAAGAGACTCTGCTTAATCATTTAGTCACCGCGGTACTTGTCATCGATAAGGATCTTAAGCCTTGTTATGCCAA
CGCCGCAGCAGAGCAACTGTTAGGTGTCGGTAGCCATAGGTTAGTCGAGCAAGCCCTGCCGGAGCATTATCAAGCACTTG
GTGTTGAAGCGCAGATCCTGAGCGATGCGGTGAAAGCCGGCCAAGGCCTTACGGTCAACACCGCCACCTTAGTGACCTTA
GATGCACAACATCATACCGTCGATCTGACGCTGATCCCGCTCGAAGATGAAGCACAACTGAGCCTACTTGAACTGAGACA
GGTCGACCAACAACGCCGGATCCACCAGCAACTCAGCCAAGATGCCCAGCAACAGGCGGCGCAATTTCTCGTGCGAAATT
TAGCCCACGAGATCAAAAATCCCCTAGGCGGCCTGCGTGGTGCTGCGCAATTGCTCTCGCGGGAATTAGACGATCCGGCA
CAAAAGGAATTTACCAATCTGATCATTGAGCAGGCCGACCGCCTGCGCAGCCTAGTGGACAGATTGCTCGGCCCGCAGCG
ACCGACCCAACATAGCCTGCACAACATTCATCAAGTGGTGCAGAAGGTCTATAAACTGGTGGAAATGGCTCTGCCCGCCA
ATATTCAGCTAAAGCGCGACTACGATCCCTCAATCCCCGATATCGAGATGGATCCCGACCAAATGCAACAAGCCGTGCTG
AATATTTTGCAGAATGCGGTGCAAGCCCTTGAGCATACAGGTGGCGAAATCCTGATCCGCACCCGCACGCAACATCAGGT
CACTATCGGTTCGCAGCGTCACAAGCTAGTGCTGACCCTGTCCATTATCGACAATGGTCCGGGCATACCACCAGAGCTGA
TGGACACACTGTTTTACCCCATGGTCACCAGTCGTGAGCAAGGTTCGGGGCTAGGCCTATCGATTGCCCATAACATTGCC
AGATTACACTCGGGCAGAATCGATTGTGTATCCAGCCCTGGGCATACCGAATTTATCATTTCGTTACCGATTTTAAGCGC
CAAATAA

Upstream 100 bases:

>100_bases
AGGTCGTTTTTACTCAATCTAGCCCTTTGATGAAGTCGAGTTGCAGCCTGCACCAAATCGGTGCAACATAATGGTGCAAC
CCTATTTTCAGGAACGGTAG

Downstream 100 bases:

>100_bases
TCAAGACAAACCAACACATACCGTATGAGGAAGCAAAATGCGAATGAGTGAACAAGTGTGGATCCTCGACGATGACAGTT
CGATACGTTGGGTGCTCGAA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQALGVEAQILSDAVKAGQGLTVNTATLVTL
DAQHHTVDLTLIPLEDEAQLSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
QKEFTNLIIEQADRLRSLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTSREQGSGLGLSIAHNIA
RLHSGRIDCVSSPGHTEFIISLPILSAK

Sequences:

>Translated_348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQALGVEAQILSDAVKAGQGLTVNTATLVTL
DAQHHTVDLTLIPLEDEAQLSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
QKEFTNLIIEQADRLRSLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTSREQGSGLGLSIAHNIA
RLHSGRIDCVSSPGHTEFIISLPILSAK
>Mature_348_residues
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQALGVEAQILSDAVKAGQGLTVNTATLVTL
DAQHHTVDLTLIPLEDEAQLSLLELRQVDQQRRIHQQLSQDAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPA
QKEFTNLIIEQADRLRSLVDRLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
NILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYPMVTSREQGSGLGLSIAHNIA
RLHSGRIDCVSSPGHTEFIISLPILSAK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=343, Percent_Identity=52.4781341107872, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788549, Length=341, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790436, Length=253, Percent_Identity=28.8537549407115, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790861, Length=277, Percent_Identity=24.5487364620939, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082128, Length=257, Percent_Identity=29.1828793774319, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=27.7056277056277, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=25.2173913043478, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=266, Percent_Identity=25.5639097744361, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789149, Length=238, Percent_Identity=23.9495798319328, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38492; Mature: 38492

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQALGVEAQIL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
SDAVKAGQGLTVNTATLVTLDAQHHTVDLTLIPLEDEAQLSLLELRQVDQQRRIHQQLSQ
HHHHHCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPAQKEFTNLIIEQADRLRSLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
NILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYP
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTSREQGSGLGLSIAHNIARLHSGRIDCVSSPGHTEFIISLPILSAK
HHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MDKETLLNHLVTAVLVIDKDLKPCYANAAAEQLLGVGSHRLVEQALPEHYQALGVEAQIL
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHH
SDAVKAGQGLTVNTATLVTLDAQHHTVDLTLIPLEDEAQLSLLELRQVDQQRRIHQQLSQ
HHHHHCCCCCEEEEEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DAQQQAAQFLVRNLAHEIKNPLGGLRGAAQLLSRELDDPAQKEFTNLIIEQADRLRSLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RLLGPQRPTQHSLHNIHQVVQKVYKLVEMALPANIQLKRDYDPSIPDIEMDPDQMQQAVL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
NILQNAVQALEHTGGEILIRTRTQHQVTIGSQRHKLVLTLSIIDNGPGIPPELMDTLFYP
HHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCEEEEEEEEECCCCCCCHHHHHHHHHH
MVTSREQGSGLGLSIAHNIARLHSGRIDCVSSPGHTEFIISLPILSAK
HHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]