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The map label for this gene is rnhB

Identifier: 116872706

GI number: 116872706

Start: 1278948

End: 1279733

Strand: Direct

Name: rnhB

Synonym: lwe1290

Alternate gene names: 116872706

Gene position: 1278948-1279733 (Clockwise)

Preceding gene: 116872705

Following gene: 116872707

Centisome position: 45.45

GC content: 34.99

Gene sequence:

>786_bases
ATGAGTGAATCTATCTCCGTTATTCGAGAGAAACTAAGTCATGTGATCTCGGAACAAGATGCTTTTTTTCAATTATGTTT
ACAAGATGAACGTAAAGGTGTTATAAAACTTATTGAATCCACACGAAAAAAATTGGAAAAAGAAGCGCAATTAATAGCTA
AGTTAGCGGAAATGAAACAATACGAAACCAATCTTTCTACACAAGGTTTTCAGTATATTGCTGGTGTGGATGAAGTTGGT
CGCGGGCCACTCGCGGGACCAGTTGTTGCTGCTGCTGTTATTTTACCAGCTGACTTTTCATTATTAGGTATTAATGATTC
TAAACAATTGAGTGAAGCAAAACGGGATATGCTTTTTGAGATAATAAAAAAAGAAGCTTTGTCAATTGGCGTGGGGATTA
TTGAGCATGACATGATTGATCAAGTAAATATTTATGAAGCAACCAAATTAGCTATGTCTGAAGCGCTAGATCAATTAAAT
CCAGCTCCAGATTTCATTTTAGTGGATGCCATGCCACTGAAATATTCTGAATCAGAATTATCGCTCATAAAAGGAGATAC
CAAAAGTATTTCTATTGCTGCCGCATCTATTATTGCCAAGGTAACTAGAGATAGAATGATGCAACACTATGATGCACTTT
ATCCAGGTTATGATTTTGCTAATAACATGGGCTATGGTACAAAAAAACACTTAAATGGTTTAGATACTATTGGAGTTTGT
CCAATTCATCGTTTAAGTTTTTCTCCAGTAAAAGAAGCAAAGTTACATTTTGAGAGTCTAAAATAG

Upstream 100 bases:

>100_bases
CAGATTATTCACGCGCAGCTGAAACAGTTGTTCGTGAAATTCGTCAACAAAAACTGGGAAGAATGTCCTTTGATTTTCCT
AACTGGGAAGATGATGCGAA

Downstream 100 bases:

>100_bases
CTAAATCGATACGAGGAAAGTGTATTTTCTAAAGAGAAAATACACTTTTTTGTGTTTAAAAAAGAATTTTAGTGATTTTA
CAAAATAAAGGAATCTTTGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSESISVIREKLSHVISEQDAFFQLCLQDERKGVIKLIESTRKKLEKEAQLIAKLAEMKQYETNLSTQGFQYIAGVDEVG
RGPLAGPVVAAAVILPADFSLLGINDSKQLSEAKRDMLFEIIKKEALSIGVGIIEHDMIDQVNIYEATKLAMSEALDQLN
PAPDFILVDAMPLKYSESELSLIKGDTKSISIAAASIIAKVTRDRMMQHYDALYPGYDFANNMGYGTKKHLNGLDTIGVC
PIHRLSFSPVKEAKLHFESLK

Sequences:

>Translated_261_residues
MSESISVIREKLSHVISEQDAFFQLCLQDERKGVIKLIESTRKKLEKEAQLIAKLAEMKQYETNLSTQGFQYIAGVDEVG
RGPLAGPVVAAAVILPADFSLLGINDSKQLSEAKRDMLFEIIKKEALSIGVGIIEHDMIDQVNIYEATKLAMSEALDQLN
PAPDFILVDAMPLKYSESELSLIKGDTKSISIAAASIIAKVTRDRMMQHYDALYPGYDFANNMGYGTKKHLNGLDTIGVC
PIHRLSFSPVKEAKLHFESLK
>Mature_260_residues
SESISVIREKLSHVISEQDAFFQLCLQDERKGVIKLIESTRKKLEKEAQLIAKLAEMKQYETNLSTQGFQYIAGVDEVGR
GPLAGPVVAAAVILPADFSLLGINDSKQLSEAKRDMLFEIIKKEALSIGVGIIEHDMIDQVNIYEATKLAMSEALDQLNP
APDFILVDAMPLKYSESELSLIKGDTKSISIAAASIIAKVTRDRMMQHYDALYPGYDFANNMGYGTKKHLNGLDTIGVCP
IHRLSFSPVKEAKLHFESLK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=48.3695652173913, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_LISW6 (A0AI76)

Other databases:

- EMBL:   AM263198
- RefSeq:   YP_849487.1
- ProteinModelPortal:   A0AI76
- SMR:   A0AI76
- STRING:   A0AI76
- GeneID:   4465520
- GenomeReviews:   AM263198_GR
- KEGG:   lwe:lwe1290
- NMPDR:   fig|386043.6.peg.1249
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   A0AI76
- ProtClustDB:   PRK00015
- BioCyc:   LWEL386043:LWE1290-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28905; Mature: 28774

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESISVIREKLSHVISEQDAFFQLCLQDERKGVIKLIESTRKKLEKEAQLIAKLAEMKQ
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETNLSTQGFQYIAGVDEVGRGPLAGPVVAAAVILPADFSLLGINDSKQLSEAKRDMLFE
HHCCCCCCCHHHHHCHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
IIKKEALSIGVGIIEHDMIDQVNIYEATKLAMSEALDQLNPAPDFILVDAMPLKYSESEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
SLIKGDTKSISIAAASIIAKVTRDRMMQHYDALYPGYDFANNMGYGTKKHLNGLDTIGVC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHCCCCCCCCCC
PIHRLSFSPVKEAKLHFESLK
CHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SESISVIREKLSHVISEQDAFFQLCLQDERKGVIKLIESTRKKLEKEAQLIAKLAEMKQ
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETNLSTQGFQYIAGVDEVGRGPLAGPVVAAAVILPADFSLLGINDSKQLSEAKRDMLFE
HHCCCCCCCHHHHHCHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHH
IIKKEALSIGVGIIEHDMIDQVNIYEATKLAMSEALDQLNPAPDFILVDAMPLKYSESEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHH
SLIKGDTKSISIAAASIIAKVTRDRMMQHYDALYPGYDFANNMGYGTKKHLNGLDTIGVC
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHCCCCCCCCCC
PIHRLSFSPVKEAKLHFESLK
CHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA