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The map label for this gene is smf [H]

Identifier: 116872707

GI number: 116872707

Start: 1279868

End: 1280728

Strand: Direct

Name: smf [H]

Synonym: lwe1291

Alternate gene names: 116872707

Gene position: 1279868-1280728 (Clockwise)

Preceding gene: 116872706

Following gene: 116872708

Centisome position: 45.48

GC content: 34.49

Gene sequence:

>861_bases
ATGAATTTTAAAGAACGAGATACGTGGCTAAAAATCGCTTCTTGTAAGTCTATTTCCGCTAAAAAAAGAGCAGTAATATG
GCAAAAGGTTTCTACTTTTGGTTGCTTTGAAATGTCGATAGAACAAATTGTGAATATATTTTTTAGTTCAGATAAAAAAG
AACAAATCAATATGGAGTTAGAAGAAGCCGATGTATATACAACAGAAGATGCAGAAATTATTTGTATTCTTGATGATAAC
TATCCAGATTTACTTAAAGAAATATATGAAGCACCACCCCTTTTATTTTGCAAAGGAAACACAGCACTACTAAAAAAACA
AGCGATGGCAATGGTTGGTACAAGAAGAATGAGTGAGTATGGTAGAAAAGCATGTGCAATGATTGCAGGAGAGCTAGCTG
AACTTAAATTAACAATTGTTAGCGGGCTTGCTCTCGGGATAGATGCAGAAGCACATAAAGCCAGCTTACGTAAAAGAGGT
GATACTATTGCAGTACTCGGCTCTGGAGTGAGTAATATTTATCCACGTTCTAATGAATTTTTAGCAAATGAAATTATAGA
ACATGGACTTCTTATCAGTGAATATTTACCGAATCAAGAAGCTAGAAGATGGCACTTTCCAGAACGCAATCGGATTATTA
GTGGACTAGCTTTAGGTACAGTAATTATCGAAGCTGCCGAGAGAAGTGGTTCTTTGATTACAGCTGATTATGCTCTGGAG
CAAAATAGACAAGTTTTTGCAGTACCAGGTAATATTTTTACTGATACATCAAGAGGTACAAATCATTTAATCCAAGAAGG
AGCGAAATTAGTCACAAATGCAAACAATATAATAGAAGAATTTTTTCAAATTAAGCCATAA

Upstream 100 bases:

>100_bases
AGAAAATACACTTTTTTGTGTTTAAAAAAGAATTTTAGTGATTTTACAAAATAAAGGAATCTTTGTACACTTGTGAGTAA
CTATCATGGAGGTGCAGAAA

Downstream 100 bases:

>100_bases
AAGTAATGAAAATAACCTTTTTAAGTGATTTGGCTTGCAATTCCTGATTTTTTGAGATAAGTTTGCTAAAGAAAGCTGAT
TAAAAACAATAAAAATAGAT

Product: Smf family DNA processing protein

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNFKERDTWLKIASCKSISAKKRAVIWQKVSTFGCFEMSIEQIVNIFFSSDKKEQINMELEEADVYTTEDAEIICILDDN
YPDLLKEIYEAPPLLFCKGNTALLKKQAMAMVGTRRMSEYGRKACAMIAGELAELKLTIVSGLALGIDAEAHKASLRKRG
DTIAVLGSGVSNIYPRSNEFLANEIIEHGLLISEYLPNQEARRWHFPERNRIISGLALGTVIIEAAERSGSLITADYALE
QNRQVFAVPGNIFTDTSRGTNHLIQEGAKLVTNANNIIEEFFQIKP

Sequences:

>Translated_286_residues
MNFKERDTWLKIASCKSISAKKRAVIWQKVSTFGCFEMSIEQIVNIFFSSDKKEQINMELEEADVYTTEDAEIICILDDN
YPDLLKEIYEAPPLLFCKGNTALLKKQAMAMVGTRRMSEYGRKACAMIAGELAELKLTIVSGLALGIDAEAHKASLRKRG
DTIAVLGSGVSNIYPRSNEFLANEIIEHGLLISEYLPNQEARRWHFPERNRIISGLALGTVIIEAAERSGSLITADYALE
QNRQVFAVPGNIFTDTSRGTNHLIQEGAKLVTNANNIIEEFFQIKP
>Mature_286_residues
MNFKERDTWLKIASCKSISAKKRAVIWQKVSTFGCFEMSIEQIVNIFFSSDKKEQINMELEEADVYTTEDAEIICILDDN
YPDLLKEIYEAPPLLFCKGNTALLKKQAMAMVGTRRMSEYGRKACAMIAGELAELKLTIVSGLALGIDAEAHKASLRKRG
DTIAVLGSGVSNIYPRSNEFLANEIIEHGLLISEYLPNQEARRWHFPERNRIISGLALGTVIIEAAERSGSLITADYALE
QNRQVFAVPGNIFTDTSRGTNHLIQEGAKLVTNANNIIEEFFQIKP

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=294, Percent_Identity=36.3945578231293, Blast_Score=159, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 31982; Mature: 31982

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKERDTWLKIASCKSISAKKRAVIWQKVSTFGCFEMSIEQIVNIFFSSDKKEQINMEL
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCCEEE
EEADVYTTEDAEIICILDDNYPDLLKEIYEAPPLLFCKGNTALLKKQAMAMVGTRRMSEY
ECCCEEECCCCEEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
GRKACAMIAGELAELKLTIVSGLALGIDAEAHKASLRKRGDTIAVLGSGVSNIYPRSNEF
HHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHCCCEEEEEECCCHHCCCCCCHH
LANEIIEHGLLISEYLPNQEARRWHFPERNRIISGLALGTVIIEAAERSGSLITADYALE
HHHHHHHHCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
QNRQVFAVPGNIFTDTSRGTNHLIQEGAKLVTNANNIIEEFFQIKP
CCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure
MNFKERDTWLKIASCKSISAKKRAVIWQKVSTFGCFEMSIEQIVNIFFSSDKKEQINMEL
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHCCEEE
EEADVYTTEDAEIICILDDNYPDLLKEIYEAPPLLFCKGNTALLKKQAMAMVGTRRMSEY
ECCCEEECCCCEEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
GRKACAMIAGELAELKLTIVSGLALGIDAEAHKASLRKRGDTIAVLGSGVSNIYPRSNEF
HHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHCCCEEEEEECCCHHCCCCCCHH
LANEIIEHGLLISEYLPNQEARRWHFPERNRIISGLALGTVIIEAAERSGSLITADYALE
HHHHHHHHCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
QNRQVFAVPGNIFTDTSRGTNHLIQEGAKLVTNANNIIEEFFQIKP
CCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]