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The map label for this gene is yafV [C]

Identifier: 116872170

GI number: 116872170

Start: 777221

End: 778111

Strand: Direct

Name: yafV [C]

Synonym: lwe0750

Alternate gene names: 116872170

Gene position: 777221-778111 (Clockwise)

Preceding gene: 116872169

Following gene: 116872175

Centisome position: 27.62

GC content: 36.48

Gene sequence:

>891_bases
ATGACTACTATAAAAATTGCATTAATCCAACAAAAAGCAGTACCAAATAATAAAGAAGCTAATTTAAAATTAGCCATCAA
ATATATAAAAGAAGCACATAAAAAAGGGGCAGATTTAGTGCTTTTCCCCGAGATGTGGTCAAACGGTTATGCGCCACCTT
TTGAAGATGCTTTTAATCATCCACTAGCTACTAGTTTTGGTGCAGAACGATTCAAGTGGTTAAATGAAGCAATTGAAGAA
GACAGTACTTATGTCTTAACGCTGAAAAAACTTGCCAAAGAGTTGCAGATTGGCATTTGCGCTACGTATTTGTCTAAAAC
GGAACAAAAACCACAAAATACTGCTATTATTATTGACCGAAAAGGAGAAATGATTTTAGACTATGCCAAAGTCCATACGT
GTGATTTTTCTTTAGAAATATTATTACAAAGTGGCGAGGGATTTAAAGTTTGTGAGTTTGATGGTATTAAGCTGGGAGTC
ATGATTTGCTATGATCGCGAGTTTCCAGAAAGCGCCAGAGTTCTCATGTTAAAGGGTGCAGAAATTATCCTCGTTCCAAA
TGCTTGTGATATGAATCCACCCAGACTTAATCAACTAAATTCTCGTGCTTTTGAAAACATGGTTGGCGTAGCGATGGCAA
ACTATCCTGGCGAAAAATGGGGCAGATCAACTGCTTTTTCCCCGATTGTTTTTGATGAAAATGGGGATTACCGCGATAAT
ACGATTATTGAAACAGATGACGTTTCAGAAGGTGTTTTTATTGCTGAATTTAATTTAGACGAGATTCGAAATTACCGGGA
AAATGAAACGTGGGGAAATGCCTACCGAAAACCGCAAACATATACAGATTTAATAAGTTCAGATGTAAAAGCGCCATTTA
AACGAAATTAA

Upstream 100 bases:

>100_bases
GATTTTTATAGACACCAGCAACTTTCGGGTTGTTGGTGTTTTTTTGTTTCCAAGAATAAATGTTATAATAACACTAAAAT
TTAATAATGGGAGGCGCCAT

Downstream 100 bases:

>100_bases
AAAAACAGAGAAGAGCAATCTCTGTTTTTTCTTTAAAAATAATAAAGCCCGGTAACAATCAGCGCAACGACCAAAATCCC
CGGAACAATATTCGCGACCC

Product: amidohydrolase, putative

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTTIKIALIQQKAVPNNKEANLKLAIKYIKEAHKKGADLVLFPEMWSNGYAPPFEDAFNHPLATSFGAERFKWLNEAIEE
DSTYVLTLKKLAKELQIGICATYLSKTEQKPQNTAIIIDRKGEMILDYAKVHTCDFSLEILLQSGEGFKVCEFDGIKLGV
MICYDREFPESARVLMLKGAEIILVPNACDMNPPRLNQLNSRAFENMVGVAMANYPGEKWGRSTAFSPIVFDENGDYRDN
TIIETDDVSEGVFIAEFNLDEIRNYRENETWGNAYRKPQTYTDLISSDVKAPFKRN

Sequences:

>Translated_296_residues
MTTIKIALIQQKAVPNNKEANLKLAIKYIKEAHKKGADLVLFPEMWSNGYAPPFEDAFNHPLATSFGAERFKWLNEAIEE
DSTYVLTLKKLAKELQIGICATYLSKTEQKPQNTAIIIDRKGEMILDYAKVHTCDFSLEILLQSGEGFKVCEFDGIKLGV
MICYDREFPESARVLMLKGAEIILVPNACDMNPPRLNQLNSRAFENMVGVAMANYPGEKWGRSTAFSPIVFDENGDYRDN
TIIETDDVSEGVFIAEFNLDEIRNYRENETWGNAYRKPQTYTDLISSDVKAPFKRN
>Mature_295_residues
TTIKIALIQQKAVPNNKEANLKLAIKYIKEAHKKGADLVLFPEMWSNGYAPPFEDAFNHPLATSFGAERFKWLNEAIEED
STYVLTLKKLAKELQIGICATYLSKTEQKPQNTAIIIDRKGEMILDYAKVHTCDFSLEILLQSGEGFKVCEFDGIKLGVM
ICYDREFPESARVLMLKGAEIILVPNACDMNPPRLNQLNSRAFENMVGVAMANYPGEKWGRSTAFSPIVFDENGDYRDNT
IIETDDVSEGVFIAEFNLDEIRNYRENETWGNAYRKPQTYTDLISSDVKAPFKRN

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=247, Percent_Identity=28.7449392712551, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=283, Percent_Identity=29.3286219081272, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 33590; Mature: 33458

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKIALIQQKAVPNNKEANLKLAIKYIKEAHKKGADLVLFPEMWSNGYAPPFEDAFNH
CCEEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCHHHHHCC
PLATSFGAERFKWLNEAIEEDSTYVLTLKKLAKELQIGICATYLSKTEQKPQNTAIIIDR
CCHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
KGEMILDYAKVHTCDFSLEILLQSGEGFKVCEFDGIKLGVMICYDREFPESARVLMLKGA
CCCEEEEEHHHEECCCEEEEEEECCCCCEEEEECCEEEEEEEEECCCCCCCCCEEEEECC
EIILVPNACDMNPPRLNQLNSRAFENMVGVAMANYPGEKWGRSTAFSPIVFDENGDYRDN
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCEEECCCCCCCCC
TIIETDDVSEGVFIAEFNLDEIRNYRENETWGNAYRKPQTYTDLISSDVKAPFKRN
CEEECCCCCCCEEEEECCHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTIKIALIQQKAVPNNKEANLKLAIKYIKEAHKKGADLVLFPEMWSNGYAPPFEDAFNH
CEEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCHHHHHCC
PLATSFGAERFKWLNEAIEEDSTYVLTLKKLAKELQIGICATYLSKTEQKPQNTAIIIDR
CCHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
KGEMILDYAKVHTCDFSLEILLQSGEGFKVCEFDGIKLGVMICYDREFPESARVLMLKGA
CCCEEEEEHHHEECCCEEEEEEECCCCCEEEEECCEEEEEEEEECCCCCCCCCEEEEECC
EIILVPNACDMNPPRLNQLNSRAFENMVGVAMANYPGEKWGRSTAFSPIVFDENGDYRDN
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCEEECCCCCCCCC
TIIETDDVSEGVFIAEFNLDEIRNYRENETWGNAYRKPQTYTDLISSDVKAPFKRN
CEEECCCCCCCEEEEECCHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]