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The map label for this gene is fadH [C]

Identifier: 116871880

GI number: 116871880

Start: 493542

End: 495536

Strand: Direct

Name: fadH [C]

Synonym: lwe0460

Alternate gene names: 116871880

Gene position: 493542-495536 (Clockwise)

Preceding gene: 116871878

Following gene: 116871881

Centisome position: 17.54

GC content: 39.2

Gene sequence:

>1995_bases
ATGACAGATTATCCGAGTATTTTTGAACCTTTAACAGTAAAACGAATGACCATTAAAAACCGTGTGATTATGCCGCCGAT
GGGGACAAACCTAGCTGGATTAAATGGCGAATTTTTAGAAGAACATATGAATTACTACGAACAACGCGCAAAAGGTGGAA
CAGGTCTGATAACGATTGAAAACGCTTGTGTAGATTTTCCTTATGGTACAAACGGGACTACGCAACTTAGAATTGATAAT
GACCAATATATTCCTGGATTCTACAAATTAACAGAACGTCTGCATAAACATGGAACTTGTGTATCTATTCAAATTAATCA
CGCCGGAGCATCTGCATATCCAGCGCGTTTGAATGGACTTCAACCAGTTTCTGCGTCAGATATACCGTCTAAAAAAGGTG
GAGCAGTTCCTCGTCCGCTTACAGTAGAAGAAATTTATGAAATCGTAAGTAAATACGGGGATGCAGCAAGACGCGCCCAA
CAAGCTGGTTTTGATGCAGTCGAAATCCATGGTGGACACTCATACTTGCTATGCCAATTTTTATCACCTCTTTATAATAA
ACGAACAGATGAATTTGGTGGAACGCCAGAAAATCGCGCACGTATTGTTAAATTAATCTTAGAAAAAGTTCGCGAGGAAG
TTGGTCCATTTTTCCCAATCGTGCTGCGTTTTAGTGCAGATGAATTTACAGAAGGTGGTAACCATTTAGAAGATATTTTG
GAACTCTTAGATTATTGCCAAGAAGAAGCGGATATTTTGAATGTATCAGCAGCAATTAATGATAATTTATATTTACAAAT
TGACCAAATGAACTTGGAAGATGGTTGGAGAAGCTACCTTGCAAAAGCAGTGAAAGATAAATTTAACAAACCAACTATTA
CTTCTGGAAACATTCGCAGTCCAAAAGCAGCGGAAAAGATTTTGTCAGAAGGATACGCTGATTTACTTGCGATGGGACGT
GGCTTAATTGCTGAGCCTAACTGGGTAAACAAAGTGGCATCTGGGCAAGAAGAAATGCTTAGAAAATGTATTTCCTGTAA
TATCGGTTGTGCGGATCACCGGATTTCGAAGTCTAAACCAATTCGTTGTACAGTAAATCCAGATATTATTCATGAAGATA
AATACCAAGAAACAAAAGTAACTCGTCCTACCAATGTAGTTGTTATTGGCGGCGGAACAGCAGGGCTTGAAGCAGCTTGT
ACGGCAGCAGAAGTTGGCTGTAACACAACGCTAATTGAAGCAAATGAACAAACTGGTGGCTTGGCGCGAGCGATTGCTAA
TCTTCCAGACAAAAGTAGAATTGCCGACTTCCCTAATTATTTAGCGAACCGAGCAGAAAAATTAACCAATTTAAAAATTA
TTACTGGCACTAAAGCTGATACTGCGCTTATTGATACATTTAATCCTGATGTGGTAGTAAATGCCACAGGCTCCAAACCA
TTACTTCCACCAATTAAAGGTTTGCTTGACGTGATTGACAAAGAAGGCAGTAAAGTTCACTCGATTTTCGGACTTATTTC
GAATATGGAAGACTTTACCGAATTTAGTAACAAGAAAGTAGCAGTTATCGGCGGAGGTGCAGTTGGACTTGATGTTGTCG
AGTACTTCTCAGAACGTGGCTCAGATGTTACAATTGTAGAAATGATGCCACTCCTTGGAAAAGACTTAGATATGATTACG
CGACTTTCCATGATGGATATTATTGAGAAAAACCATGTGGATGTGCAAACAGAAACAGCTCTAACAGAAGTAGCAGCTGA
TCATTTCAAAGTGAAGCATGACGGAGTAGACGCCGAGATTCCATTTGATTATGGCTTTGTTTGTCTAGGAATGCGACCAG
AACGCCCACTTATGGAAGAACTGACAGCATATGGAAAAGAAAAACAAATTGAAATTGTTAATATTGGCGATAGCGTAGCG
ACAAGAAAAATCTTGGAAGGCGTTCGCGAAGGAAGAAATATTTTAACTACATTAGAAAAAATAGGCTCTTTGTAA

Upstream 100 bases:

>100_bases
TAGTCCTGTCTTATAATGAAAGAGGAATAAGTTTGTGCATTATTGAACGTGAATAATGTAACAAAAGATTAGCTGAGATT
ATAGTCGCAGGAGGCTTATT

Downstream 100 bases:

>100_bases
TACGAATTGGCAGAGCGGATTCCGCTTTGCCACATTCGCTCTTTATATAAGTTCACATTTTCACAAATAAACCTTAATTT
GGAAAGGAACGATGTTATTA

Product: NADH:flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDN
DQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGAVPRPLTVEEIYEIVSKYGDAARRAQ
QAGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGTPENRARIVKLILEKVREEVGPFFPIVLRFSADEFTEGGNHLEDIL
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGR
GLIAEPNWVNKVASGQEEMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYQETKVTRPTNVVVIGGGTAGLEAAC
TAAEVGCNTTLIEANEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKIITGTKADTALIDTFNPDVVVNATGSKP
LLPPIKGLLDVIDKEGSKVHSIFGLISNMEDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMIT
RLSMMDIIEKNHVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELTAYGKEKQIEIVNIGDSVA
TRKILEGVREGRNILTTLEKIGSL

Sequences:

>Translated_664_residues
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDN
DQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGAVPRPLTVEEIYEIVSKYGDAARRAQ
QAGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGTPENRARIVKLILEKVREEVGPFFPIVLRFSADEFTEGGNHLEDIL
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGR
GLIAEPNWVNKVASGQEEMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYQETKVTRPTNVVVIGGGTAGLEAAC
TAAEVGCNTTLIEANEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKIITGTKADTALIDTFNPDVVVNATGSKP
LLPPIKGLLDVIDKEGSKVHSIFGLISNMEDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMIT
RLSMMDIIEKNHVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELTAYGKEKQIEIVNIGDSVA
TRKILEGVREGRNILTTLEKIGSL
>Mature_663_residues
TDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIENACVDFPYGTNGTTQLRIDND
QYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGLQPVSASDIPSKKGGAVPRPLTVEEIYEIVSKYGDAARRAQQ
AGFDAVEIHGGHSYLLCQFLSPLYNKRTDEFGGTPENRARIVKLILEKVREEVGPFFPIVLRFSADEFTEGGNHLEDILE
LLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRSPKAAEKILSEGYADLLAMGRG
LIAEPNWVNKVASGQEEMLRKCISCNIGCADHRISKSKPIRCTVNPDIIHEDKYQETKVTRPTNVVVIGGGTAGLEAACT
AAEVGCNTTLIEANEQTGGLARAIANLPDKSRIADFPNYLANRAEKLTNLKIITGTKADTALIDTFNPDVVVNATGSKPL
LPPIKGLLDVIDKEGSKVHSIFGLISNMEDFTEFSNKKVAVIGGGAVGLDVVEYFSERGSDVTIVEMMPLLGKDLDMITR
LSMMDIIEKNHVDVQTETALTEVAADHFKVKHDGVDAEIPFDYGFVCLGMRPERPLMEELTAYGKEKQIEIVNIGDSVAT
RKILEGVREGRNILTTLEKIGSL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=543, Percent_Identity=30.0184162062615, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=29.8913043478261, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17565138, Length=316, Percent_Identity=29.4303797468354, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=309, Percent_Identity=28.8025889967638, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=341, Percent_Identity=26.6862170087977, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17564188, Length=309, Percent_Identity=28.4789644012945, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540738, Length=342, Percent_Identity=25.7309941520468, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17566914, Length=350, Percent_Identity=26, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=308, Percent_Identity=26.6233766233766, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321973, Length=226, Percent_Identity=31.4159292035398, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 72960; Mature: 72829

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIE
CCCCCCHHCCHHHEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
NACVDFPYGTNGTTQLRIDNDQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGL
CCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
QPVSASDIPSKKGGAVPRPLTVEEIYEIVSKYGDAARRAQQAGFDAVEIHGGHSYLLCQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPLYNKRTDEFGGTPENRARIVKLILEKVREEVGPFFPIVLRFSADEFTEGGNHLEDIL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCHHHHHHH
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRS
HHHHHHHHHHHEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKAAEKILSEGYADLLAMGRGLIAEPNWVNKVASGQEEMLRKCISCNIGCADHRISKSKP
CHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPDIIHEDKYQETKVTRPTNVVVIGGGTAGLEAACTAAEVGCNTTLIEANEQTGG
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
LARAIANLPDKSRIADFPNYLANRAEKLTNLKIITGTKADTALIDTFNPDVVVNATGSKP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCEEEECCCCCC
LLPPIKGLLDVIDKEGSKVHSIFGLISNMEDFTEFSNKKVAVIGGGAVGLDVVEYFSERG
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
SDVTIVEMMPLLGKDLDMITRLSMMDIIEKNHVDVQTETALTEVAADHFKVKHDGVDAEI
CCEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCCCC
PFDYGFVCLGMRPERPLMEELTAYGKEKQIEIVNIGDSVATRKILEGVREGRNILTTLEK
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGSL
HCCC
>Mature Secondary Structure 
TDYPSIFEPLTVKRMTIKNRVIMPPMGTNLAGLNGEFLEEHMNYYEQRAKGGTGLITIE
CCCCCHHCCHHHEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
NACVDFPYGTNGTTQLRIDNDQYIPGFYKLTERLHKHGTCVSIQINHAGASAYPARLNGL
CCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
QPVSASDIPSKKGGAVPRPLTVEEIYEIVSKYGDAARRAQQAGFDAVEIHGGHSYLLCQF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LSPLYNKRTDEFGGTPENRARIVKLILEKVREEVGPFFPIVLRFSADEFTEGGNHLEDIL
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCHHHHHHH
ELLDYCQEEADILNVSAAINDNLYLQIDQMNLEDGWRSYLAKAVKDKFNKPTITSGNIRS
HHHHHHHHHHHEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PKAAEKILSEGYADLLAMGRGLIAEPNWVNKVASGQEEMLRKCISCNIGCADHRISKSKP
CHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC
IRCTVNPDIIHEDKYQETKVTRPTNVVVIGGGTAGLEAACTAAEVGCNTTLIEANEQTGG
EEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCH
LARAIANLPDKSRIADFPNYLANRAEKLTNLKIITGTKADTALIDTFNPDVVVNATGSKP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCEEEECCCCCC
LLPPIKGLLDVIDKEGSKVHSIFGLISNMEDFTEFSNKKVAVIGGGAVGLDVVEYFSERG
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCC
SDVTIVEMMPLLGKDLDMITRLSMMDIIEKNHVDVQTETALTEVAADHFKVKHDGVDAEI
CCEEHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCCCC
PFDYGFVCLGMRPERPLMEELTAYGKEKQIEIVNIGDSVATRKILEGVREGRNILTTLEK
CCCCCEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGSL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]