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The map label for this gene is thiM [H]

Identifier: 116871707

GI number: 116871707

Start: 312424

End: 313233

Strand: Direct

Name: thiM [H]

Synonym: lwe0287

Alternate gene names: 116871707

Gene position: 312424-313233 (Clockwise)

Preceding gene: 116871706

Following gene: 116871708

Centisome position: 11.1

GC content: 47.53

Gene sequence:

>810_bases
ATGTTTGATTTTATGACGTTGGAAAAGGTGCAAGAAAGAGGGCCGTTGGTACATAATATTACAAACATCGTGGTTGCAAA
TGACTCGGCAAACGGATTGCTGGCGATTGGAGCATCACCAATTATGGCCTCTGCGAAACAAGAAATGGACGAACTGGCGA
AAATGGCGGATGTGCTCGTGATTAATATTGGTATGCTGGACGGCGAGTTGGTGGAAGCGATGAAAATTGCTGGACGTGCG
GCAAATTTTGCGGAGACGCAGGTTGTTCTAGACCCGGTCGGTGTTGGCGCAACTTCATATCGACGTAAAGTGGTGCAGGA
ATTGTTGGCAGAAATTCAGTTTGCGGCGATTCGCGGAAATGCAGGAGAGCTTGCGGCGATTGCTGGAGAAGCATGGGAAG
CGAAAGGCGTGGATGCGGGTGTAGGTTCGGCGGATGTGCTCGCTATCGCTGAAAAAGTAGCAAAGGAATGGAACACGGTT
GTGATTATTAGTGGTGAAGTGGATGTGATTTCAGACGGAACTCGCTTTGCAAAAGTGGCGAATGGTAGCGCGCTTCTGCC
AAGAATTACTGGTTCTGGTTGTTTGCTAAGTGCGGTTTGTGGTAGTTTTATCGCAGTTCAGGATGATGTTTTTCGTGCTA
GTGTTGAAGCGTGCGTGAGTTATGCAGTGGCTTCTGAATATGCGGAAATGGAGTTAGAAAGAAAACTCCCAGGCTCTTTT
CGACCATTATTTTTGGATGCGCTGGCTAGTTGGTCGGTCGAAAAAACGCGTGCTAAAGCCAAAATCCTAGAAAGTGGTGA
ACACAAATGA

Upstream 100 bases:

>100_bases
CTTGCTCTCATGAAGCAGGCTTTTCACATCAGTGTGGAAATGGAATGGGCGTTTTTTGATATGGCTTATAAAAAACAAAA
TTGGCGTGGGAGTGTGAATT

Downstream 100 bases:

>100_bases
TTTTCAAACAAGTGTTAACGATAGCTGGTTCAGATTCAGGTGGCGGCGCGGGAATCCAAGCAGATATTAAGACATTTCAA
GAGCGCAGAACCTTTGGAAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFDFMTLEKVQERGPLVHNITNIVVANDSANGLLAIGASPIMASAKQEMDELAKMADVLVINIGMLDGELVEAMKIAGRA
ANFAETQVVLDPVGVGATSYRRKVVQELLAEIQFAAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLAIAEKVAKEWNTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFIAVQDDVFRASVEACVSYAVASEYAEMELERKLPGSF
RPLFLDALASWSVEKTRAKAKILESGEHK

Sequences:

>Translated_269_residues
MFDFMTLEKVQERGPLVHNITNIVVANDSANGLLAIGASPIMASAKQEMDELAKMADVLVINIGMLDGELVEAMKIAGRA
ANFAETQVVLDPVGVGATSYRRKVVQELLAEIQFAAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLAIAEKVAKEWNTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFIAVQDDVFRASVEACVSYAVASEYAEMELERKLPGSF
RPLFLDALASWSVEKTRAKAKILESGEHK
>Mature_269_residues
MFDFMTLEKVQERGPLVHNITNIVVANDSANGLLAIGASPIMASAKQEMDELAKMADVLVINIGMLDGELVEAMKIAGRA
ANFAETQVVLDPVGVGATSYRRKVVQELLAEIQFAAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLAIAEKVAKEWNTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFIAVQDDVFRASVEACVSYAVASEYAEMELERKLPGSF
RPLFLDALASWSVEKTRAKAKILESGEHK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=249, Percent_Identity=39.7590361445783, Blast_Score=163, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325042, Length=271, Percent_Identity=32.4723247232472, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28399; Mature: 28399

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFMTLEKVQERGPLVHNITNIVVANDSANGLLAIGASPIMASAKQEMDELAKMADVLV
CCCCHHHHHHHHCCCEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHE
INIGMLDGELVEAMKIAGRAANFAETQVVLDPVGVGATSYRRKVVQELLAEIQFAAIRGN
EEECCCCHHHHHHHHHHCCCCCCHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECC
AGELAAIAGEAWEAKGVDAGVGSADVLAIAEKVAKEWNTVVIISGEVDVISDGTRFAKVA
CCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEEEC
NGSALLPRITGSGCLLSAVCGSFIAVQDDVFRASVEACVSYAVASEYAEMELERKLPGSF
CCCEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPLFLDALASWSVEKTRAKAKILESGEHK
CHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDFMTLEKVQERGPLVHNITNIVVANDSANGLLAIGASPIMASAKQEMDELAKMADVLV
CCCCHHHHHHHHCCCEEECCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHE
INIGMLDGELVEAMKIAGRAANFAETQVVLDPVGVGATSYRRKVVQELLAEIQFAAIRGN
EEECCCCHHHHHHHHHHCCCCCCHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECC
AGELAAIAGEAWEAKGVDAGVGSADVLAIAEKVAKEWNTVVIISGEVDVISDGTRFAKVA
CCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEEEC
NGSALLPRITGSGCLLSAVCGSFIAVQDDVFRASVEACVSYAVASEYAEMELERKLPGSF
CCCEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RPLFLDALASWSVEKTRAKAKILESGEHK
CHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]