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The map label for this gene is ispD [H]

Identifier: 116871619

GI number: 116871619

Start: 212234

End: 212926

Strand: Direct

Name: ispD [H]

Synonym: lwe0199

Alternate gene names: 116871619

Gene position: 212234-212926 (Clockwise)

Preceding gene: 116871618

Following gene: 116871620

Centisome position: 7.54

GC content: 38.82

Gene sequence:

>693_bases
ATGAATTATGAGCTGATTTTTTTAGCGGCAGGACAAGGTAAACGAATGAATGCGCAAAAAAATAAAATGTGGCTAGATCT
AGTTGGAGAACCTATTTTCATTCATGCTTTACGTCCTTTTCTAGCCGATAACCGTTGTTCAAAAGTGATAGTTGTATGCC
AAGAAACAGAACGAAAACATGTAGAAAAATTAATGCAGCAGTTACATGTCGCAAAAAACCGCATAGAAATTGTTAAGGGC
GGAAAAGAACGTCAAGATAGTGTGGCGGCCGGACTTGGAAGATGTGGCAATGAGTCCGTAGTTTTAGTTCATGATGGAGC
TAGACCATTTGTAACGATAGATATCATTGATCGGTTACTAAGCGGTGTGAAAGAAAATAAAGCTGCTATATGTGCTGTAA
AAGTGAAAGATACCGTGAAACGGGTTATTGATAATCTTGTTCAAGAGACGGTAGATCGCGAAAACCTTTGGCAGATTCAA
ACTCCGCAAGCTTTTGAACTGCATATTTTACAAAAAGCACACCAGCTTGCAAAAGAAGAGCAGTTTTTAGGAACAGACGA
AGCAAGTTTAGTAGAGCGAATTCCTTGGCCGGTTGCTACTGTTCAAGGAAGCTATTATAATATTAAATTAACGACTCCAG
AAGATATGCCGCTTGCTAAGGCGATTTTAGGAGAACTTGGGGAAAATTATTAA

Upstream 100 bases:

>100_bases
ATTAATGAGACCATTCAAGTCGAGGTAACTAGTGTGCTTCAAACATCAGCAGGAAGAATGATTTTTGCTAAACCATCCTG
AATAAAAGGAGTAAAAGGGC

Downstream 100 bases:

>100_bases
AGGAGTGAATAATTGTGAGTGAAACAAAACGAGTACGTGTGCGTTATGCGCCGAGTCCAACTGGTTTTTTGCATATTGGA
AATGCACGTACTGCCTTATT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1; MEP cytidylyltransferase 1; MCT 1 [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNYELIFLAAGQGKRMNAQKNKMWLDLVGEPIFIHALRPFLADNRCSKVIVVCQETERKHVEKLMQQLHVAKNRIEIVKG
GKERQDSVAAGLGRCGNESVVLVHDGARPFVTIDIIDRLLSGVKENKAAICAVKVKDTVKRVIDNLVQETVDRENLWQIQ
TPQAFELHILQKAHQLAKEEQFLGTDEASLVERIPWPVATVQGSYYNIKLTTPEDMPLAKAILGELGENY

Sequences:

>Translated_230_residues
MNYELIFLAAGQGKRMNAQKNKMWLDLVGEPIFIHALRPFLADNRCSKVIVVCQETERKHVEKLMQQLHVAKNRIEIVKG
GKERQDSVAAGLGRCGNESVVLVHDGARPFVTIDIIDRLLSGVKENKAAICAVKVKDTVKRVIDNLVQETVDRENLWQIQ
TPQAFELHILQKAHQLAKEEQFLGTDEASLVERIPWPVATVQGSYYNIKLTTPEDMPLAKAILGELGENY
>Mature_230_residues
MNYELIFLAAGQGKRMNAQKNKMWLDLVGEPIFIHALRPFLADNRCSKVIVVCQETERKHVEKLMQQLHVAKNRIEIVKG
GKERQDSVAAGLGRCGNESVVLVHDGARPFVTIDIIDRLLSGVKENKAAICAVKVKDTVKRVIDNLVQETVDRENLWQIQ
TPQAFELHILQKAHQLAKEEQFLGTDEASLVERIPWPVATVQGSYYNIKLTTPEDMPLAKAILGELGENY

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=219, Percent_Identity=36.986301369863, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25906; Mature: 25906

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYELIFLAAGQGKRMNAQKNKMWLDLVGEPIFIHALRPFLADNRCSKVIVVCQETERKH
CCEEEEEEECCCCCCCCCHHHCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHH
VEKLMQQLHVAKNRIEIVKGGKERQDSVAAGLGRCGNESVVLVHDGARPFVTIDIIDRLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHH
SGVKENKAAICAVKVKDTVKRVIDNLVQETVDRENLWQIQTPQAFELHILQKAHQLAKEE
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
QFLGTDEASLVERIPWPVATVQGSYYNIKLTTPEDMPLAKAILGELGENY
HHCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYELIFLAAGQGKRMNAQKNKMWLDLVGEPIFIHALRPFLADNRCSKVIVVCQETERKH
CCEEEEEEECCCCCCCCCHHHCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHH
VEKLMQQLHVAKNRIEIVKGGKERQDSVAAGLGRCGNESVVLVHDGARPFVTIDIIDRLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHH
SGVKENKAAICAVKVKDTVKRVIDNLVQETVDRENLWQIQTPQAFELHILQKAHQLAKEE
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHH
QFLGTDEASLVERIPWPVATVQGSYYNIKLTTPEDMPLAKAILGELGENY
HHCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]