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The map label for this gene is gltX [H]

Identifier: 116871620

GI number: 116871620

Start: 212941

End: 214416

Strand: Direct

Name: gltX [H]

Synonym: lwe0200

Alternate gene names: 116871620

Gene position: 212941-214416 (Clockwise)

Preceding gene: 116871619

Following gene: 116871621

Centisome position: 7.57

GC content: 37.4

Gene sequence:

>1476_bases
GTGAGTGAAACAAAACGAGTACGTGTGCGTTATGCGCCGAGTCCAACTGGTTTTTTGCATATTGGAAATGCACGTACTGC
CTTATTTAACTATTTATTTGCAAGACATAATGATGGAGACTTTATTATTCGGATTGAGGATACGGATGCGAAACGTAACG
TAGCTGATGGCGAAGAAAGTCAAATGAAAAATTTGAAATGGCTAGGTATGGATTGGGATGAAGGTGTTGATGTTCCTGGA
AAATATGGTCCTTATCGTCAATCTGAACGCCAATCTATTTACGAACCGCTTATTCAACAATTATTGGACGAAGGCTTGGC
TTACAAATGTTATTGCACGGAAGAAGAATTAGAAGCAGAACGTGAAAAACAAAAAGCTAATAATGAAATGCCTCGTTATA
GTGGTAAATGCCGTCATTTAACAAAAGAACAACAAGCTGAAAAAGAAGCGCAAGGTTTTAAACCAAGCATTCGTTTCAAA
GTACCAGCTAATGAAACAATTACTTTTAACGATATGGTTAAAGATGATGTTTCTTTTGAATCAAATGGTATTGGTGATTT
TGTTATTGCGAAAAAAGACGGAATCCCAACGTACAACTTTGCTGTAGCTGTGGATGATCATTTAATGGAAATTTCGCATG
TTCTTCGCGGTGATGATCATATTTCAAATACACCAAAACAAATTTTGATATACAATGCTTTTGGTTGGGAGCCACCAACT
TTCGGTCATATGACGCTCATTGTGAATGAAAGTAGACGAAAACTAAGTAAGCGTGACGGTTCGATTATTCAATTTATCGA
ACAATATCGTGATTTAGGATATTTACCAGAAGCATTATTTAACTTCATTGCGATGCTTGGTTGGTCTCCTGAAGGGGAAG
AAGAAATTTTCTCCAAAGAAGAATTTATTAAGATGTTTGATCCAAAACGTTTATCTAAATCACCAGCATTATTTGATAAT
GTCAAATTGACTTGGGTAAATAACCAATATGTGAAAAAATTACCGCTAAATGATGTAGTGGAGCTTTCCTTACCACATTT
ACAAAAAGCGGGGGTTGTGTCTGCTGATTTAGATCAAGCGGAACTTGACTGGGTGCACAAATTAGTTTCACTTTATCATG
AACAAATGAGTTATGGTGCAGAGATTGTGCCACTTTCTGAGATGTTCTTTGCGGATGCAGAGGCGATTACTTTTGATGAA
GAAGAAAAAGCTGTTCTTGCGGAAGATACAGTCCCAACTGTTATTTCGGCCTTCAAAAAAGAGTTAGAAGCACTAGAAGT
TCTTGAAGCTGCGGAAGTAAAAGCGGCAATCAAGCGTGTCCAAAAAGAAACGGGAGTAAAAGGCAAAGGATTATTTATGC
CAATTCGGATTGTGACTACTGGCGAAATGCATGGTCCTGAATTGCCGCTTGCAATCGAAGTTCTTGGTCGTGAAAAAGTG
CTTAATCGTATGGATACATGGTTGAAAAATAATTAA

Upstream 100 bases:

>100_bases
GAAGCTATTATAATATTAAATTAACGACTCCAGAAGATATGCCGCTTGCTAAGGCGATTTTAGGAGAACTTGGGGAAAAT
TATTAAAGGAGTGAATAATT

Downstream 100 bases:

>100_bases
TTTTGAATGGAAGCATCTCGGCAATTTGCGGGGTGCTTTTTTATTTTGATATATTTCAGTTGCAACGAATTTTATTGCTG
TTATAATAGAACTAAATATA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNVADGEESQMKNLKWLGMDWDEGVDVPG
KYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELEAEREKQKANNEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFK
VPANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPT
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDN
VKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSADLDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAEAITFDE
EEKAVLAEDTVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
LNRMDTWLKNN

Sequences:

>Translated_491_residues
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNVADGEESQMKNLKWLGMDWDEGVDVPG
KYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELEAEREKQKANNEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFK
VPANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPT
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDN
VKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSADLDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAEAITFDE
EEKAVLAEDTVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
LNRMDTWLKNN
>Mature_490_residues
SETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNVADGEESQMKNLKWLGMDWDEGVDVPGK
YGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELEAEREKQKANNEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKV
PANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPTF
GHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDNV
KLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSADLDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAEAITFDEE
EKAVLAEDTVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKVL
NRMDTWLKNN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=336, Percent_Identity=40.7738095238095, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI4826960, Length=304, Percent_Identity=24.6710526315789, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=37.888198757764, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI226510925, Length=262, Percent_Identity=32.824427480916, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=33.6776859504132, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17511007, Length=262, Percent_Identity=23.2824427480916, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=507, Percent_Identity=33.7278106508876, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324742, Length=311, Percent_Identity=24.1157556270096, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=211, Percent_Identity=27.4881516587678, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=34.4689378757515, Blast_Score=285, Evalue=7e-77,
Organism=Drosophila melanogaster, GI17864488, Length=300, Percent_Identity=25.3333333333333, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56052; Mature: 55921

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNVADGEES
CCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHH
QMKNLKWLGMDWDEGVDVPGKYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELEAE
HHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
REKQKANNEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKVPANETITFNDMVKDDVSFE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCC
SNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPT
CCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCC
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKE
CCCEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EFIKMFDPKRLSKSPALFDNVKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSADLDQA
HHHHHCCCHHCCCCCCEECCEEEEEECCHHHHHCCCCHHHHCCCCCHHHCCCEECCCCHH
ELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAEAITFDEEEKAVLAEDTVPTVISAFKK
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCEEEECCCCCCEEECCCHHHHHHHHHH
ELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHHH
LNRMDTWLKNN
HHHHHHHHCCC
>Mature Secondary Structure 
SETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNVADGEES
CCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHH
QMKNLKWLGMDWDEGVDVPGKYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELEAE
HHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
REKQKANNEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKVPANETITFNDMVKDDVSFE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCC
SNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPT
CCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCC
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKE
CCCEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EFIKMFDPKRLSKSPALFDNVKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSADLDQA
HHHHHCCCHHCCCCCCEECCEEEEEECCHHHHHCCCCHHHHCCCCCHHHCCCEECCCCHH
ELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAEAITFDEEEKAVLAEDTVPTVISAFKK
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCEEEECCCCCCEEECCCHHHHHHHHHH
ELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHHH
LNRMDTWLKNN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]