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The map label for this gene is radA [H]

Identifier: 116871617

GI number: 116871617

Start: 209634

End: 211007

Strand: Direct

Name: radA [H]

Synonym: lwe0197

Alternate gene names: 116871617

Gene position: 209634-211007 (Clockwise)

Preceding gene: 116871616

Following gene: 116871618

Centisome position: 7.45

GC content: 40.03

Gene sequence:

>1374_bases
ATGGCTAAGGCAAAAAGGACGACCAAATTTGTATGTCAGGCATGTGGATATGAATCGCCAAAATGGATGGGTAAATGTCC
GAATTGCAACGAGTGGAATCAGATGGTGGAAGCATTAGAACCATCGAAAAAATCGCGCTCAGCTTTTAATCATACAGGAG
AACCATCAAAAGCAACACCGATTACTCAAATTGCGAGTGAAGAAGAGGCAAGAGTAGAGACAAATATGCCTGAATTAAAT
AGAGTTTTGGGTGGCGGTGTGGTTCCTGGTTCCATGGTTCTTGTTGGTGGAGATCCAGGTATTGGTAAATCAACGCTTTT
ACTTCAAGTGTCAGCACAACTTACACTTACTAATAAAAAAGTTCTCTATATTTCTGGGGAAGAATCAATTAAGCAAACAA
AACTTCGAGCAGAACGTTTACAAGTTTCAGGTGATAATTTGTATGTTTATGCAGAAACAAATTTAGAAGCGGTTCAAGAA
ACAATTGATTTTGTAAAGCCTGATTTTGTAGTAATTGATTCTATTCAAACAGTTTATCATCCAGATGTAACGAGCGCTGC
GGGAAGCGTGTCGCAAGTTAGAGAATGTACAGCAGCTTTAATGCGAATTGCTAAAATGCAAAATATCGCTATTTTTATTG
TTGGTCATGTGACGAAGGAAGGTGCAATTGCAGGTCCTCGTTTACTTGAGCATATGGTAGATACAGTGCTTTATTTTGAA
GGAGAGCGTCATCATGCATACCGAATTTTACGCGCAGTGAAAAACCGTTTTGGCTCAACAAATGAAATGGGGATTTTTGA
AATGCGTGATATTGGTCTTGTAGAAGTTGCCAATCCATCGGAGGTTTTCTTAGAGGAGCGTTTAGAAGGAGCTTCGGGTT
CAACCGTTGTAGCTTCAATGGAAGGAACGCGACCAGTGTTAGTAGAAATTCAAGCACTTGTATCGCCAACTATGTTTGGA
AATGCGAAAAGAATGGCCACCGGGATTGATTATAACAAGGTATCATTGATTATGGCTGTTTTAGAAAAAAGAGTAGGACT
CATGTTACAAAACCAAGATGCATATTTGAAAGCGGCTGGTGGGGTTAAGTTAGACGAACCGGCAGTTGATTTAGCAGTTG
CTGTTAGCGTAGCTTCCAGTTATCGTGATAAGCCGACGAGGAGTACGGATTGTTTTATTGGTGAGCTTGGTTTAACAGGA
GAAATTAGACGTGTGGCAAGAATTGAGCAACGTGTACAAGAAGCCGCAAAACTTGGCTTTAAACGAATTTTTATTCCAAA
AAATAATGAAGGTACTTGGAAAATACCAAAAGACGTGCAAGTGGTTGGTGTGGAAACGATTGGAGAAGCATTGAAGAAGG
CTTTACCAGATTGA

Upstream 100 bases:

>100_bases
AAATACTAGATTAGCGCTGAAAAAACGAATTTGGCGAAAAGGCTTAACGCGAACAGATGATCTGTGGTAAAATGAAACAT
ATGTATGGAGGGATGTAGAA

Downstream 100 bases:

>100_bases
AAATAAATACTTTAATGATTCCATGAGTACTATTTGCGGTATAGTCTTTTAATAGTGTAAAATAGTGCTATATGTATAGT
TGGTAATAGTGCGATTATAT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MAKAKRTTKFVCQACGYESPKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASEEEARVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTAALMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDIGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGTWKIPKDVQVVGVETIGEALKKALPD

Sequences:

>Translated_457_residues
MAKAKRTTKFVCQACGYESPKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASEEEARVETNMPELN
RVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQE
TIDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTAALMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHAYRILRAVKNRFGSTNEMGIFEMRDIGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFG
NAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTG
EIRRVARIEQRVQEAAKLGFKRIFIPKNNEGTWKIPKDVQVVGVETIGEALKKALPD
>Mature_456_residues
AKAKRTTKFVCQACGYESPKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATPITQIASEEEARVETNMPELNR
VLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKKVLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQET
IDFVKPDFVVIDSIQTVYHPDVTSAAGSVSQVRECTAALMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFEG
ERHHAYRILRAVKNRFGSTNEMGIFEMRDIGLVEVANPSEVFLEERLEGASGSTVVASMEGTRPVLVEIQALVSPTMFGN
AKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAGGVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGE
IRRVARIEQRVQEAAKLGFKRIFIPKNNEGTWKIPKDVQVVGVETIGEALKKALPD

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=44.2013129102845, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49960; Mature: 49829

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKRTTKFVCQACGYESPKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
ITQIASEEEARVETNMPELNRVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKK
HHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEEECCE
VLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQETIDFVKPDFVVIDSIQTVYH
EEEEECCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDVTSAAGSVSQVRECTAALMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
CCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHEEC
GERHHAYRILRAVKNRFGSTNEMGIFEMRDIGLVEVANPSEVFLEERLEGASGSTVVASM
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
EGTRPVLVEIQALVSPTMFGNAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAG
CCCCCHHEEHHHHHCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEECC
GVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGEIRRVARIEQRVQEAAKLGF
CCEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
KRIFIPKNNEGTWKIPKDVQVVGVETIGEALKKALPD
EEEEEECCCCCCEECCCCEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKAKRTTKFVCQACGYESPKWMGKCPNCNEWNQMVEALEPSKKSRSAFNHTGEPSKATP
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCC
ITQIASEEEARVETNMPELNRVLGGGVVPGSMVLVGGDPGIGKSTLLLQVSAQLTLTNKK
HHHHCCCCHHHHHCCCHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEEECCE
VLYISGEESIKQTKLRAERLQVSGDNLYVYAETNLEAVQETIDFVKPDFVVIDSIQTVYH
EEEEECCHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDVTSAAGSVSQVRECTAALMRIAKMQNIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
CCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHEEC
GERHHAYRILRAVKNRFGSTNEMGIFEMRDIGLVEVANPSEVFLEERLEGASGSTVVASM
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCCCCEEEEEC
EGTRPVLVEIQALVSPTMFGNAKRMATGIDYNKVSLIMAVLEKRVGLMLQNQDAYLKAAG
CCCCCHHEEHHHHHCCHHCCCCHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCEEEECC
GVKLDEPAVDLAVAVSVASSYRDKPTRSTDCFIGELGLTGEIRRVARIEQRVQEAAKLGF
CCEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
KRIFIPKNNEGTWKIPKDVQVVGVETIGEALKKALPD
EEEEEECCCCCCEECCCCEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]