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The map label for this gene is yiaE [C]

Identifier: 116871498

GI number: 116871498

Start: 74820

End: 75776

Strand: Direct

Name: yiaE [C]

Synonym: lwe0076

Alternate gene names: 116871498

Gene position: 74820-75776 (Clockwise)

Preceding gene: 116871496

Following gene: 116871499

Centisome position: 2.66

GC content: 41.69

Gene sequence:

>957_bases
ATGAAAGCGAGCGTATTTGTAGCAGGGAAATTACTACCGGAAACGATGAAAGCTCTTGATAAATGGGACGTTAAGGCTTA
TACCAGTCAAGAAAACATTACAGAAGAGGAGTTAATAAAAAGTGTAACGGAAGTGGATGCAATTATTTGTCCGCTCTCTT
CACCAATTACTGCAAAAGTATTGGAATCAGCAAAAAATCTTAAAATTGTAGCAAATATCGGCGCCGGTTTTGATAATATT
GATGTAAAAAAAGCGCAGAAACTCGGGATTGCAGTAACAAATACGCCAGATGTATCAACTGAAGCGACGGCAGAACTGAC
GCTTGGACTAATTTTGGCGGTGGCTCGGAGAATTTCAGAAGGTGACCGTTTGTGTCGTGAAACACCAGAGCAATTTAAAG
GCTGGGCGCCAACGTTCTTTTTAGGTACAGAACTAAGGGGTAAAACATTGGGAATTATTGGTTTAGGAAGAATTGGTCAA
TCCGTGGCAAAACGTGCAGCCGCATTTGGAATGAAAATCATATATTCCGGACACCATCCGAAAGAAGCGGCGAAAGAATG
GAATGCCGAGTTCGTGAGTCAAGAAGAATTATTGAAACGTAGCGATGTTGTGACTATTCACGCCGCATACAATCCGAGCT
TGAAGCATCTGTTGAACGAAACAACACTAAAAACGATGAAGTCGAGTGCATTTTTGATTAATGCTGCTAGAGGACCTGTT
GTAGAAGAAGTTGCACTTATTAATGCGCTGAAAACAGGTATTATCGCGGGTGCTGCGCTTGATGTATTTGAGTTTGAACC
TAAAATTGGTCCCGAGTTAGGCAAATTAGATAATGTTGTTTTAACACCGCATATTGGTAACGCAACCGTCGAAACCCGCG
CAGAAATGGGGCGCATGGCTATTTCAAATGTGGAAGCAGTTTTAGCAGGAAACAGCCCAATCCATTCTGTTTATTAA

Upstream 100 bases:

>100_bases
ACGTATTTTGACTGGAAAAGAAGCGGAAAATCGTTTACCTTTCATAAAAAACGGATTACAATAAATGTAAGATGAAAATG
GTATTTGAGGAGTGAACGAG

Downstream 100 bases:

>100_bases
GACTAATAAATATAGCCATAGCAAGAAGCTATGGCTGTTTTTTTCTACCAAATAATGGTATAATATTTCTGGTAGAATAG
AGCAACGGGTTGTTAGCTCA

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKASVFVAGKLLPETMKALDKWDVKAYTSQENITEEELIKSVTEVDAIICPLSSPITAKVLESAKNLKIVANIGAGFDNI
DVKKAQKLGIAVTNTPDVSTEATAELTLGLILAVARRISEGDRLCRETPEQFKGWAPTFFLGTELRGKTLGIIGLGRIGQ
SVAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYNPSLKHLLNETTLKTMKSSAFLINAARGPV
VEEVALINALKTGIIAGAALDVFEFEPKIGPELGKLDNVVLTPHIGNATVETRAEMGRMAISNVEAVLAGNSPIHSVY

Sequences:

>Translated_318_residues
MKASVFVAGKLLPETMKALDKWDVKAYTSQENITEEELIKSVTEVDAIICPLSSPITAKVLESAKNLKIVANIGAGFDNI
DVKKAQKLGIAVTNTPDVSTEATAELTLGLILAVARRISEGDRLCRETPEQFKGWAPTFFLGTELRGKTLGIIGLGRIGQ
SVAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYNPSLKHLLNETTLKTMKSSAFLINAARGPV
VEEVALINALKTGIIAGAALDVFEFEPKIGPELGKLDNVVLTPHIGNATVETRAEMGRMAISNVEAVLAGNSPIHSVY
>Mature_318_residues
MKASVFVAGKLLPETMKALDKWDVKAYTSQENITEEELIKSVTEVDAIICPLSSPITAKVLESAKNLKIVANIGAGFDNI
DVKKAQKLGIAVTNTPDVSTEATAELTLGLILAVARRISEGDRLCRETPEQFKGWAPTFFLGTELRGKTLGIIGLGRIGQ
SVAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYNPSLKHLLNETTLKTMKSSAFLINAARGPV
VEEVALINALKTGIIAGAALDVFEFEPKIGPELGKLDNVVLTPHIGNATVETRAEMGRMAISNVEAVLAGNSPIHSVY

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=307, Percent_Identity=36.8078175895765, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI6912396, Length=313, Percent_Identity=37.3801916932907, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI61743967, Length=259, Percent_Identity=43.2432432432432, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4557497, Length=259, Percent_Identity=43.2432432432432, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=38.8888888888889, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=38.8888888888889, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI145580575, Length=270, Percent_Identity=38.8888888888889, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI87082289, Length=259, Percent_Identity=40.5405405405405, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789279, Length=292, Percent_Identity=33.5616438356164, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1787645, Length=293, Percent_Identity=29.0102389078498, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788660, Length=264, Percent_Identity=28.030303030303, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=275, Percent_Identity=38.1818181818182, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25147481, Length=284, Percent_Identity=34.5070422535211, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=240, Percent_Identity=39.5833333333333, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322116, Length=283, Percent_Identity=37.1024734982332, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=291, Percent_Identity=37.1134020618557, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321253, Length=281, Percent_Identity=27.7580071174377, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=249, Percent_Identity=27.710843373494, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=161, Percent_Identity=29.8136645962733, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325144, Length=168, Percent_Identity=26.1904761904762, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571528, Length=299, Percent_Identity=42.809364548495, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24585514, Length=292, Percent_Identity=35.6164383561644, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28574282, Length=292, Percent_Identity=35.6164383561644, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=40.0749063670412, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=40.0749063670412, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=40.0749063670412, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=40.0749063670412, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI28574284, Length=292, Percent_Identity=35.6164383561644, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45552429, Length=292, Percent_Identity=35.6164383561644, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45551003, Length=292, Percent_Identity=35.6164383561644, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI62472511, Length=267, Percent_Identity=40.0749063670412, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19921140, Length=292, Percent_Identity=36.3013698630137, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28574286, Length=287, Percent_Identity=35.191637630662, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24585516, Length=288, Percent_Identity=32.9861111111111, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 34198; Mature: 34198

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASVFVAGKLLPETMKALDKWDVKAYTSQENITEEELIKSVTEVDAIICPLSSPITAKV
CCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHH
LESAKNLKIVANIGAGFDNIDVKKAQKLGIAVTNTPDVSTEATAELTLGLILAVARRISE
HHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
GDRLCRETPEQFKGWAPTFFLGTELRGKTLGIIGLGRIGQSVAKRAAAFGMKIIYSGHHP
HHHHHHCCHHHHCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEECCCCC
KEAAKEWNAEFVSQEELLKRSDVVTIHAAYNPSLKHLLNETTLKTMKSSAFLINAARGPV
HHHHHHHCHHHCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
VEEVALINALKTGIIAGAALDVFEFEPKIGPELGKLDNVVLTPHIGNATVETRAEMGRMA
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
ISNVEAVLAGNSPIHSVY
HHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKASVFVAGKLLPETMKALDKWDVKAYTSQENITEEELIKSVTEVDAIICPLSSPITAKV
CCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHH
LESAKNLKIVANIGAGFDNIDVKKAQKLGIAVTNTPDVSTEATAELTLGLILAVARRISE
HHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
GDRLCRETPEQFKGWAPTFFLGTELRGKTLGIIGLGRIGQSVAKRAAAFGMKIIYSGHHP
HHHHHHCCHHHHCCCCCEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCEEEEECCCCC
KEAAKEWNAEFVSQEELLKRSDVVTIHAAYNPSLKHLLNETTLKTMKSSAFLINAARGPV
HHHHHHHCHHHCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
VEEVALINALKTGIIAGAALDVFEFEPKIGPELGKLDNVVLTPHIGNATVETRAEMGRMA
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
ISNVEAVLAGNSPIHSVY
HHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA