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The map label for this gene is recF [H]

Identifier: 116871427

GI number: 116871427

Start: 4868

End: 5980

Strand: Direct

Name: recF [H]

Synonym: lwe0005

Alternate gene names: 116871427

Gene position: 4868-5980 (Clockwise)

Preceding gene: 116871426

Following gene: 116871428

Centisome position: 0.17

GC content: 38.1

Gene sequence:

>1113_bases
ATGCATTTAGAAAGCATTGTTTTAAGAAATTTCCGCAATTATGAAAACTTAGAACTAGAATTTTCCCCGTCTGTGAATGT
TTTTCTTGGAGAAAATGCACAAGGTAAAACTAATCTTTTGGAAGCTGTACTTATGCTTGCTCTTGCAAAATCACATCGGA
CAACCAATGATAAAGATTTTATTATGTGGGAAAAAGAAGAAGCCAAAATGGAAGGGCGAGTAGTAAAGCGCGGGCAAACA
GTCCCTTTAGAGCTTGCTATCACCCAAAAAGGCAAGCGAGCTAAGGTTAACCATCTGGAACAAAAGAAACTGAGCCAATA
TGTTGGTAACTTAAATGTGGTTATTTTTGCACCAGAAGATTTATCGCTTGTTAAAGGTGCTCCAGGAATTAGACGCCGAT
TTTTGAATATGGAAATTGGACAAATGCAGCCGATTTATTTGCATAATTTAAGTGAATATCAGCGGATTTTACAACAACGG
AACCAATACTTGAAGATGCTACAAATGAAACGTAAAGTAGATCCGATGTTGCTCGATATTTTGACAGAGCAATTCGCAGA
TGTCGCTATTAATTTGACGAAAAGGCGCGCTGATTTTATTCAAAAATTAGAAGCATATGCAGCGCCAATTCATCACCAAA
TTTCTCGAGGATTAGAAACACTTAAAATCGAGTATAAAGCTTCCGTTACACTGAATGGGGACGATCCCGACACGTGGAAA
GCGGATTTACTTCAAAAAATGGAATCAATCAAACAAAGAGAAATCGACCGTGGTGTCACACTTGTTGGGCCACATCGGGA
TGATTCTCTGTTTTATATTAATGGGCAAAATGTGCAAGATTTTGGTTCACAAGGTCAACAGCGGACAACGGCACTTTCTG
TAAAATTAGCAGAAATTGACCTAATCCATGAAGAAACTGGAGAATATCCAGTTCTACTTCTTGATGATGTACTTAGTGAA
CTAGATGATTACCGTCAATCGCATTTGCTTGGAGCTATTGAAGGAAAAGTACAAACCTTTGTAACGACAACAAGCACGAG
CGGGATTGATCATGACACGCTCAAACAAGCAACGACATTTTATGTAGAAAAAGGTACAGTAAAAAAATCCTAA

Upstream 100 bases:

>100_bases
CGGAGAGCAAGATAATCGCCGGGGGAAAAAGCTTCGTAATGGCGATGTAATCTTAGTTCCTGGCGTTGGAAAAGTAAAAA
TTGAGCAAGGGTAATAGCAG

Downstream 100 bases:

>100_bases
TCTAGGTTAATATAATTTGATGAAAAAGAAAGTGCGGTGGAATAATTAATGTCAGAAGAAAATATTACAAATGTACATGA
AAGTGCTTCAGATTATAACG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRVVKRGQT
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPMLLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASVTLNGDDPDTWK
ADLLQKMESIKQREIDRGVTLVGPHRDDSLFYINGQNVQDFGSQGQQRTTALSVKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHDTLKQATTFYVEKGTVKKS

Sequences:

>Translated_370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRVVKRGQT
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPMLLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASVTLNGDDPDTWK
ADLLQKMESIKQREIDRGVTLVGPHRDDSLFYINGQNVQDFGSQGQQRTTALSVKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHDTLKQATTFYVEKGTVKKS
>Mature_370_residues
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDFIMWEKEEAKMEGRVVKRGQT
VPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPEDLSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQR
NQYLKMLQMKRKVDPMLLDILTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASVTLNGDDPDTWK
ADLLQKMESIKQREIDRGVTLVGPHRDDSLFYINGQNVQDFGSQGQQRTTALSVKLAEIDLIHEETGEYPVLLLDDVLSE
LDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHDTLKQATTFYVEKGTVKKS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=372, Percent_Identity=25.5376344086022, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42253; Mature: 42253

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00617 RECF_1 ; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDF
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IMWEKEEAKMEGRVVKRGQTVPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPED
EEEEHHHHHHCCHHEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQRNQYLKMLQMKRKVDPMLLDI
CHHHCCCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASVTLNGDDPDTWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEECCCCCCHHH
ADLLQKMESIKQREIDRGVTLVGPHRDDSLFYINGQNVQDFGSQGQQRTTALSVKLAEID
HHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCHHHHHCCCCCHHHEEEEEEHHEE
LIHEETGEYPVLLLDDVLSELDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHDTLKQATTF
EEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHE
YVEKGTVKKS
EEECCCCCCC
>Mature Secondary Structure
MHLESIVLRNFRNYENLELEFSPSVNVFLGENAQGKTNLLEAVLMLALAKSHRTTNDKDF
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCE
IMWEKEEAKMEGRVVKRGQTVPLELAITQKGKRAKVNHLEQKKLSQYVGNLNVVIFAPED
EEEEHHHHHHCCHHEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
LSLVKGAPGIRRRFLNMEIGQMQPIYLHNLSEYQRILQQRNQYLKMLQMKRKVDPMLLDI
CHHHCCCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTEQFADVAINLTKRRADFIQKLEAYAAPIHHQISRGLETLKIEYKASVTLNGDDPDTWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEEEEEEECCCCCCHHH
ADLLQKMESIKQREIDRGVTLVGPHRDDSLFYINGQNVQDFGSQGQQRTTALSVKLAEID
HHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCHHHHHCCCCCHHHEEEEEEHHEE
LIHEETGEYPVLLLDDVLSELDDYRQSHLLGAIEGKVQTFVTTTSTSGIDHDTLKQATTF
EEHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHE
YVEKGTVKKS
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]