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The map label for this gene is gyrB [H]

Identifier: 116871428

GI number: 116871428

Start: 6029

End: 7969

Strand: Direct

Name: gyrB [H]

Synonym: lwe0006

Alternate gene names: 116871428

Gene position: 6029-7969 (Clockwise)

Preceding gene: 116871427

Following gene: 116871429

Centisome position: 0.21

GC content: 39.05

Gene sequence:

>1941_bases
ATGTCAGAAGAAAATATTACAAATGTACATGAAAGTGCTTCAGATTATAACGAAGATCAAATACAAGTACTAGAAGGCCT
AGAAGCTGTAAGAAAAAGACCTGGTATGTACATTGGTTCAACTAGTCAACGTGGACTTCATCACCTTGTATGGGAAATCG
TTGATAACGCAATTGATGAAGCTCTTGCTGGTTTTTGTACAGAAATTGAAATTACAATTGAAGCTGATAATAGCATAACT
GTTCGCGATAACGGACGTGGAATTCCAACTGGGATTAACGAAAAAATCGGTCGTCCAACAGTAGAAGTTATTTTTACTGT
TCTACATGCTGGTGGTAAATTTGGCGGCGGCGGATATAAAGTATCAGGTGGACTTCATGGAGTTGGTGCATCAGTAGTTA
ATGCTCTTTCAACATCTCTTGAAGTATATGTTCACCGTGAAGGACAAAAGTATTACCAACGTTTTGAACGTGGTGATGTA
GTGATGGATATGGAAGTACAAGGGGAAACAGATTATCGTGGAACCATCGTTCATTTTACGCCAGATCCACAAATTTTCAC
TGAAACAACAGAATTTGATTTTGATACGCTTCGTACTCGTACGCGCGAACTTGCTTTCTTGAATCGTGGCTTAACAATTT
CTATTGAAGACAAACGCGAAGAACACAAAGTTCGTAAAGATTTCCACTATGAAGGCGGAATTCGTTCTTACGTGGAGCAT
TTAAATAAAGCCAAAGATGTTATCCATGAGCCACCAATTTATTTGGAAGGTGAACGCGATGATATTATGGTCGAGATTTC
CATGCAGTACAACACTGGATTTTCAAGCAATATCATTTCATTTGCGAATAATATTCATACGTATGAAGGCGGGACTCACG
AATCTGGTTTTAAAACAGCTTTAACACGTGTTATTAATGACTATGCGCGTCGTAATAAAGTGTTTAAAGATAGTGATGAC
AACCTTTCTGGTGAAGATGTTCGTGAAGGTTTAACCGCAATTATTTCTATCAAACATCCGGATCCGCAGTTTGAAGGACA
AACAAAAACAAAACTTGGAAATTCAGAAGCTCGTTCTATCACGGATAAGCTGTTTTCTGAGGCTTTAAATAAATTTATGA
TGGAAAACCCAGATGTTGCTAAAAAAATCGTTGAGAAAGGCGTGGTGGCTTCTCGTGCACGTCTGGCTGCTAAGCGCGCG
CGTGAAGTTGCTCGTAAAAGCAGTGGTCTAGAAATTTCTAGTTTACCAGGTAAGCTAGCAGACTGTTCTTCACGTAACCC
TGAAATCAGCGAACTTTACATCGTTGAGGGTGACTCAGCTGGTGGTTCAGCTAAACAAGGTCGGGACCGTTTATTCCAAG
CGATTTTACCGATTCGTGGGAAAATTTTGAACGTGGAAAAAGCACGGTTGGATCGTATTTTAGCTAACGAAGAAATCCGA
ACTATTTTTACAGCTATGGGTACTGGTTTTGGTGGAGATTTCGACGTTTCTAAATCTCGTTACCACAAACTTATTATCAT
GACTGATGCCGACGTAGATGGTGCACATATTCGTACGCTACTTCTTACGCTATTTTATCGTTATATGCGTCCTCTGCTTG
ATGCAGGTTATATTTATATTGCGCAACCACCGCTTTATCAAATTAAGCACGGTAAGCAAATTGAGTACGTATATAGTGAT
GGACAATTAGAAGATTATCTAGCAAGCCTTGATAAAGACACTAAATACAGCATTCAACGATATAAAGGTCTTGGAGAAAT
GAATCCAGAACAACTATGGGATACAACAATGAATCCAGAACACCGTACACTACTTCAAGTGAATATCAAAGACGCTATTG
ATGCTGATGAAACTTTTGAAATGTTGATGGGTGACCGTGTGGAACCTCGTCGTAAATTCATTGAAGAAAACGCACAATAC
GTTAAAAACTTGGATGTTTAA

Upstream 100 bases:

>100_bases
CAAACAAGCAACGACATTTTATGTAGAAAAAGGTACAGTAAAAAAATCCTAATCTAGGTTAATATAATTTGATGAAAAAG
AAAGTGCGGTGGAATAATTA

Downstream 100 bases:

>100_bases
TAAATATTTCCCGGGTAATAATTGGGTCATTAATTGTTTTTAAAAGGTTTGAACTAGATTCAGCCTTACTTTTGAAAGGG
AGGTTTCTCTAACAATGGCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MSEENITNVHESASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSIT
VRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDV
VMDMEVQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKVFKDSDD
NLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRA
REVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSD
GQLEDYLASLDKDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEENAQY
VKNLDV

Sequences:

>Translated_646_residues
MSEENITNVHESASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSIT
VRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDV
VMDMEVQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKVFKDSDD
NLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRA
REVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSD
GQLEDYLASLDKDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEENAQY
VKNLDV
>Mature_645_residues
SEENITNVHESASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDEALAGFCTEIEITIEADNSITV
RDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVV
MDMEVQGETDYRGTIVHFTPDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEHL
NKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTALTRVINDYARRNKVFKDSDDN
LSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSITDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAR
EVARKSSGLEISSLPGKLADCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIRT
IFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYIAQPPLYQIKHGKQIEYVYSDG
QLEDYLASLDKDTKYSIQRYKGLGEMNPEQLWDTTMNPEHRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEENAQYV
KNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=622, Percent_Identity=27.8135048231511, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI19913406, Length=616, Percent_Identity=26.461038961039, Blast_Score=137, Evalue=4e-32,
Organism=Escherichia coli, GI48994957, Length=566, Percent_Identity=53.886925795053, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=39.5238095238095, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17535065, Length=616, Percent_Identity=26.1363636363636, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=594, Percent_Identity=23.9057239057239, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=624, Percent_Identity=27.4038461538462, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17136538, Length=677, Percent_Identity=25.8493353028065, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72838; Mature: 72707

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEENITNVHESASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDE
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALAGFCTEIEITIEADNSITVRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYK
HHHCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVVMDMEVQGETDYRGTIVHFT
ECCCCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEC
PDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTA
HHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHCCEECCCCCCCCHHHHH
LTRVINDYARRNKVFKDSDDNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHH
TDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAREVARKSSGLEISSLPGKLA
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYI
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYQIKHGKQIEYVYSDGQLEDYLASLDKDTKYSIQRYKGLGEMNPEQLWDTTMNPE
ECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCC
HRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEENAQYVKNLDV
CCEEEEEECHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEENITNVHESASDYNEDQIQVLEGLEAVRKRPGMYIGSTSQRGLHHLVWEIVDNAIDE
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHH
ALAGFCTEIEITIEADNSITVRDNGRGIPTGINEKIGRPTVEVIFTVLHAGGKFGGGGYK
HHHCCEEEEEEEEECCCEEEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCEE
VSGGLHGVGASVVNALSTSLEVYVHREGQKYYQRFERGDVVMDMEVQGETDYRGTIVHFT
ECCCCCHHHHHHHHHHHHCEEEEEEECCHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEC
PDPQIFTETTEFDFDTLRTRTRELAFLNRGLTISIEDKREEHKVRKDFHYEGGIRSYVEH
CCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHH
LNKAKDVIHEPPIYLEGERDDIMVEISMQYNTGFSSNIISFANNIHTYEGGTHESGFKTA
HHHHHHHHCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHHCCEECCCCCCCCHHHHH
LTRVINDYARRNKVFKDSDDNLSGEDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARSI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHCCCCHHHHHH
TDKLFSEALNKFMMENPDVAKKIVEKGVVASRARLAAKRAREVARKSSGLEISSLPGKLA
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHH
DCSSRNPEISELYIVEGDSAGGSAKQGRDRLFQAILPIRGKILNVEKARLDRILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
TIFTAMGTGFGGDFDVSKSRYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPLLDAGYIYI
HHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPLYQIKHGKQIEYVYSDGQLEDYLASLDKDTKYSIQRYKGLGEMNPEQLWDTTMNPE
ECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCC
HRTLLQVNIKDAIDADETFEMLMGDRVEPRRKFIEENAQYVKNLDV
CCEEEEEECHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]