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The map label for this gene is trmE [H]

Identifier: 116691178

GI number: 116691178

Start: 3478729

End: 3480123

Strand: Reverse

Name: trmE [H]

Synonym: Bcen2424_3161

Alternate gene names: 116691178

Gene position: 3480123-3478729 (Counterclockwise)

Preceding gene: 116691180

Following gene: 116691174

Centisome position: 99.89

GC content: 69.39

Gene sequence:

>1395_bases
ATGCTCGCTACCGATTCCGATCCGATCGTCGCCATTGCCACTGCTGCCGGCCGGGGCGGCATCGGGGTCGTCCGCGTGTC
GTTCGGGCGCGGCGGCGAGGCGGCCGCGCTGCCGTTGATCGACGCGTTGTGCGGGCAAAAGCTCGCGCCGCGCCATGCGA
GCTACGTGCCGTTCCTCGACGCGCATGGCGCGCCGCTCGACCGCGGGATCGCGCTGTATTTCCCGGCGCCGCATTCGTAC
ACCGGCGAGCACGTGCTCGAGTTGCAGGGGCATGGCGGGCCGATCGTGATGCAGCTGCTGCTGCAGCGCTGCCTGGATGC
GGGGCGCGGCTTCGGGCTACGGCTGGCGGAGCCGGGCGAGTTCACGCGGCGCGCGTTCCTGAACGACAAGCTCGATCTCG
CGCAGGCCGAGGCCGTCGCGGACCTGATCGAGGCGAGCACCGAGGCGGCCGCGCGCTCGGCCGGGCGTTCGCTCGACGGC
GCGTTCTCGCGGCAGATCCATGCGCTCGTCGACGACGTGATCACGCTGCGGATGCTGGTGGAGGCGACGCTCGATTTTCC
GGAGGAGGAAATCGACTTCCTCGAAGCGGCCGACGCGCGCGGCAAGCTCGCGAAGATCCGCGCGCAGCTCGCGCACGTGC
TCGGCGATGCGCGGCAGGGCGCGCTGTTGCGCGAAGGGCTGTCGGTCGTGCTCGCGGGGCAGCCGAACGTCGGCAAGTCG
TCGCTGCTGAATGCGCTGGCGGGCGCCGAGTTGGCGATCGTCACGCCGATCGCCGGCACGACCCGCGACAAGGTCGCGCA
GACGATCCAGGTCGAGGGGATTCCGCTGCACATCATCGATACGGCCGGGTTGCGCGAGACGGAGGACGAAGTCGAACGGA
TCGGCATTGCGCGCACGTGGAGCGAGATCGAGCGCGCGGACGTCGTGCTGCATCTGCTCGATTCGCGCACGGGGATGACG
GCGGACGACGAAACGATCGCCGCGCGGTTTCCGGCCGGCGTGCCGGTGGTGCGCGTGCTGAACAAGACGGATCTGACCGG
CGTGCCGGCGTGCGTCGAGCATCCGGCGGCGGAAGGCGATCTGACCGAGGTGCACCTGTCGGCGAAGCGCGGTGACGGGA
TCGACATGCTGCGCGCGGAGCTGCTGCGGATTGCGGGGTGGCAGGCGGGGGCCGAGGGGGTGTATCTGGCGCGCGAGCGG
CATCTGATCGCGCTGCGGGCCGCGCAGGAACATCTCGCGCAGGCGGCGAATCACGCGGAGCAGCGGGCGCAGTCGCTCGA
TCTGTTTGCCGAAGAGCTGCGGCTCGCACAGGAGCAGCTCAATGCGATTACCGGGGAGTTCACGTCGGATGATCTGCTGG
GGGTAATTTTCAGCAGGTTTTGTATCGGGAAGTGA

Upstream 100 bases:

>100_bases
AGCCGGCACGCTACAATGCCGGCGTTCCGGTGCCCTAACTGTTGCGCGGGCGCACCATTGACCCGCTGATTACCCGATTT
CCCTGATTCCTTCGATTCCC

Downstream 100 bases:

>100_bases
GCGGGCACTGAGCCGTATGTTGCCGCTGCCAGCGGCGTGCCATCGAGTACGAACGGCATGGGCGCTCACGCATCGTCATG
GCCGGATGCTGAAACGAATT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDAHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDETIAARFPAGVPVVRVLNKTDLTGVPACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAANHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDAHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDETIAARFPAGVPVVRVLNKTDLTGVPACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAANHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
>Mature_464_residues
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLDAHGAPLDRGIALYFPAPHSY
TGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRQIHALVDDVITLRMLVEATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTWSEIERADVVLHLLDSRTGMT
ADDETIAARFPAGVPVVRVLNKTDLTGVPACVEHPAAEGDLTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARER
HLIALRAAQEHLAQAANHAEQRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=477, Percent_Identity=38.1551362683438, Blast_Score=229, Evalue=5e-60,
Organism=Homo sapiens, GI193082993, Length=467, Percent_Identity=37.4732334047109, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=35.5029585798817, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=54.6808510638298, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI87082120, Length=117, Percent_Identity=41.8803418803419, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=29.247311827957, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=503, Percent_Identity=29.4234592445328, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI45550813, Length=489, Percent_Identity=32.719836400818, Blast_Score=185, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49421; Mature: 49421

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEC
AHGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRTGMTADDETIAARFPAGVPVVRVLNKTDLTGVPACVEHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCC
LTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARERHLIALRAAQEHLAQAANHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATDSDPIVAIATAAGRGGIGVVRVSFGRGGEAAALPLIDALCGQKLAPRHASYVPFLD
CCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEC
AHGAPLDRGIALYFPAPHSYTGEHVLELQGHGGPIVMQLLLQRCLDAGRGFGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRQIHALVDDVITLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAKIRAQLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQVEGIPLHIIDTAGLRETEDEVERIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHH
SEIERADVVLHLLDSRTGMTADDETIAARFPAGVPVVRVLNKTDLTGVPACVEHPAAEGD
HHHHHHHHHHHHHHCCCCCCCCCCHHEECCCCCCHHHHHHCCCCCCCCCHHHCCCCCCCC
LTEVHLSAKRGDGIDMLRAELLRIAGWQAGAEGVYLARERHLIALRAAQEHLAQAANHAE
CEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHH
QRAQSLDLFAEELRLAQEQLNAITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA