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The map label for this gene is potI [H]

Identifier: 116691017

GI number: 116691017

Start: 3310528

End: 3311367

Strand: Direct

Name: potI [H]

Synonym: Bcen2424_2999

Alternate gene names: 116691017

Gene position: 3310528-3311367 (Clockwise)

Preceding gene: 116691016

Following gene: 116691018

Centisome position: 95.02

GC content: 65.0

Gene sequence:

>840_bases
ATGATCAAGCCGAGCAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTCCTGTACATCCCGATCATCAGCCT
CGTCGTGTACTCGTTCAACGAGTCGAAGCTGGTCACGGTGTGGTCGGGCTTCTCGCTGAAGTGGTACGCGGCGCTGCTGG
ACGACGACGAGCTGCTGACCGCCGCGTGGCTGTCGCTGAAGATCGGCCTGCTGACGGCCACCGCGTCGGTCGTGATCGGC
ACGTGGGCCGGCTTCGTGCTCGCGCGCTTCGGCCGCTTCAAGGGCTTCACGCTGTACACGGGGATGATCAACGCGCCGCT
GGTGATTCCGGAGGTGATCCAGGGGATCTCGCTGCTGCTGCTGTTCGTCGCGCTCGAGCAGATGTTCGGCTGGCCGAAGG
GCCGCGGGATGCTGACGATCTGGATCGGCCACGTGATGCTGTGCGTGTCGTACGTGGCGATCATCGTGCAGTCGCGCGTG
AAGGAGATGAACAAGTCGCTCGAGGAAGCCGCGCTCGACCTCGGCGCCACGCCGCTGAAGGTGTTCTTCGTGGTGACGCT
GCCGCTGATCTCGCAGGCGTTGCTGTCGGGGTGGCTGCTGTCGTTCACGCTGTCGATCGACGATCTGGTGCTGTCGGCGT
TCCTGTCCGGGCCGGGGTCGACGACGCTGCCGCTGGTGGTGTTCTCGCGCGTGCGGCTGGGGCTGAACCCGGAGATGAAT
GCACTGGCGACGCTGTTCATCACGGCCGTGACGATCGGCGTGATCGTCGTGAACCGGATGATGATTGCGCGCGAGCGTCG
CCGGATGGCCGACTTGAAGGCCGCGTTCGCGGCGGCTTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGCTGCTGTTGCTGGTGCCGATGGCGCTGTTCCAGTACTACCAGGTC
AAGGAACTGGGAGACGCGAA

Downstream 100 bases:

>100_bases
CCGCTCGTCTCCCGCTTCGAGGATAACAAGCACTGCAGACAAGCAACACGCGCTGCCCCAGGCGGGGCGCGCGATTCGAT
CCCGATTTGACAGGCGCACG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMLTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRMADLKAAFAAA

Sequences:

>Translated_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMLTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRMADLKAAFAAA
>Mature_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMLTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRMADLKAAFAAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=270, Percent_Identity=56.6666666666667, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1787368, Length=240, Percent_Identity=37.9166666666667, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787714, Length=245, Percent_Identity=31.8367346938775, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788764, Length=229, Percent_Identity=25.3275109170306, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30453; Mature: 30453

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLDDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMLTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNRMMIARERRRMADLKAAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYAALLDDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHCCCCHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMLTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNRMMIARERRRMADLKAAFAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]