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The map label for this gene is potH [H]

Identifier: 116691016

GI number: 116691016

Start: 3309545

End: 3310531

Strand: Direct

Name: potH [H]

Synonym: Bcen2424_2998

Alternate gene names: 116691016

Gene position: 3309545-3310531 (Clockwise)

Preceding gene: 116691015

Following gene: 116691017

Centisome position: 95.0

GC content: 64.64

Gene sequence:

>987_bases
ATGAGAACCTCCGCTTCCACCCCTTCCGCGCCGGTGTCGGCCGGCGCCGCGCGCACCGGCGCGGGTCATGCCCGCCGCGG
CCGCTTCGCCGCGCTGTCGCGCTTCCTGCCGTCGGGCCGCAGCGTCGCGATCGGCGTGCCGTTCCTGTGGCTGGCCGTGT
TCTTCGCGCTGCCGTTCGTGCTGGTGCTGAAGATCAGCTTTGCCGACCAGGTGATGGGCATCCCGCCGTACACGTCGCTC
GTCGAGATCAAGGACGGCGTCGTGCACTTCGCGCTGCAGATGTCGCACTACGCGTTCCTGCTGCAGGACGACCTGTACAT
CGCGACCTATCTCAGCTCGCTGAAGATGGCCGCCGTGTCGACGGTGCTGTGTTTGCTGATCGGCTATCCGATGGCGTACT
ACATCGCGCGCTCGGAGCCGAACCGCCGCAACGTGCTGATGATGGCCGTGATGCTGCCGTTCTGGACCTCGTTCCTGATC
CGCGTGTACGCATGGATCGGCATCCTGAAGGACGACGGCCTGCTGAACCACACGCTGATCGCGCTCGGCATCATCCACAC
GCCGCTGCGGCTCTACCACAGCGATGCGGGCGTCTATATCGGCATGGTCTATTCGTACCTGCCGTTCATGGTGATGCCGC
TCTATGCGCACCTCGTGAAGATGGACCTGACGCTGCTCGAAGCCGCGTACGACCTCGGCGCGAAACCGTGGGTCGCGTTC
ACGCGCATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCCGGCAGCCTGCTGGTGTTCATCCCGGCGGTGGGCGAGTA
CGTGATCCCGGAGCTGCTCGGCGGCGCCGACACGCTGATGATCGGCCGCGTGATGTGGGATGAATTCTTCAACAACATGG
ACTGGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGCTGCTGTTGCTGGTGCCGATGGCGCTGTTCCAGTACTACCAG
GTCAAGGAACTGGGAGACGCGAAATGA

Upstream 100 bases:

>100_bases
TCGCACCTGATGAACGACGGCGCACCGGCGTGGAACGACGACGTGTTCGTGTCGTGGTCGCCGGCCAGCGGCGTCGTGCT
GACGCAGTGAGGACGCCACG

Downstream 100 bases:

>100_bases
TCAAGCCGAGCAAACCGCTGTCGACGGGCGTTCTCGCCTTCGGCTTCCTGTTCCTGTACATCCCGATCATCAGCCTCGTC
GTGTACTCGTTCAACGAGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRTSASTPSAPVSAGAARTGAGHARRGRFAALSRFLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEIKDGVVHFALQMSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELGDAK

Sequences:

>Translated_328_residues
MRTSASTPSAPVSAGAARTGAGHARRGRFAALSRFLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEIKDGVVHFALQMSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELGDAK
>Mature_328_residues
MRTSASTPSAPVSAGAARTGAGHARRGRFAALSRFLPSGRSVAIGVPFLWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEIKDGVVHFALQMSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVLLLLVPMALFQYYQ
VKELGDAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=288, Percent_Identity=55.2083333333333, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=38.7096774193548, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1787713, Length=229, Percent_Identity=32.7510917030568, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36246; Mature: 36246

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSASTPSAPVSAGAARTGAGHARRGRFAALSRFLPSGRSVAIGVPFLWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVEIKDGVVHFALQMSHYAFLLQDDLYIATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHCCCHHHHHHHHHHHHHHHH
TVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHTLI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVLLLLVPMALFQYYQVKELGDAK
HHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTSASTPSAPVSAGAARTGAGHARRGRFAALSRFLPSGRSVAIGVPFLWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVEIKDGVVHFALQMSHYAFLLQDDLYIATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHCCCHHHHHHHHHHHHHHHH
TVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHTLI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVLLLLVPMALFQYYQVKELGDAK
HHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]