Click here to switch to the map view.

The map label for this gene is trmL [H]

Identifier: 116690871

GI number: 116690871

Start: 3151472

End: 3151990

Strand: Direct

Name: trmL [H]

Synonym: Bcen2424_2851

Alternate gene names: 116690871

Gene position: 3151472-3151990 (Clockwise)

Preceding gene: 116690870

Following gene: 116690877

Centisome position: 90.46

GC content: 68.98

Gene sequence:

>519_bases
ATGCACGCGCGCGTTGCGCCGCTCCGGCCGCCCCAGCCAAACCGAAACATGTTCAACGTCGTTCTCGTCGCCCCCGAAAT
TCCGCCGAACACCGGCAACGTGATCCGCCTGTGCGCGAACACCGGCGCGCATCTGCACCTGATCGAGCCGCTCGGCTTTC
CGCTCGACGACGCGCGGATGCGCCGCGCCGGCCTCGACTATCACGAGTATGCGCAGATGCGCGTGCACCGCGACTGGGAT
GCGTTCGTCGCCGCCGAAACGCCCGATCCCGCGCGGATGTTCGCGTTCACGACGCGCGGCTCGGGCCGCTTCCACGATCA
TGCGTTCCTGTCCGGCGACTGGTTCGTGTTCGGCTCGGAAACGCGCGGCCTGCCGGCCGAGCTGCTCGAACGCTTCCCGA
ACGAGCAGCGCGTACGCCTGCCGATGCGGCCGGACAACCGCAGCCTGAACCTGTCGAACACGGTCGCGGTGGTCGTGTTC
GAGGCGTGGCGCCAGGCCGGCTTCGAAGGCGGCGCCTGA

Upstream 100 bases:

>100_bases
GGCGCCGCGCGCGTGACGAATCTGGTTGCGCTGCGCACCGCCAGGGATTAATTAGATAGAGAGGCCGCCCGCATGCGGCC
GTCACGAACCGGCCAGCCGG

Downstream 100 bases:

>100_bases
CACGCGCCGGGCGTAGCCCGCGGCGAAGGCGTCAGGTTCGTGCGAGCCGCGCGCGGTACAGGTCGTAGATGTCGGGGGAA
AAGCACGCGAACACCACGCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MHARVAPLRPPQPNRNMFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARMRRAGLDYHEYAQMRVHRDWD
AFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSETRGLPAELLERFPNEQRVRLPMRPDNRSLNLSNTVAVVVF
EAWRQAGFEGGA

Sequences:

>Translated_172_residues
MHARVAPLRPPQPNRNMFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARMRRAGLDYHEYAQMRVHRDWD
AFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSETRGLPAELLERFPNEQRVRLPMRPDNRSLNLSNTVAVVVF
EAWRQAGFEGGA
>Mature_172_residues
MHARVAPLRPPQPNRNMFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARMRRAGLDYHEYAQMRVHRDWD
AFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSETRGLPAELLERFPNEQRVRLPMRPDNRSLNLSNTVAVVVF
EAWRQAGFEGGA

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=57.6923076923077, Blast_Score=196, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19428; Mature: 19428

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHARVAPLRPPQPNRNMFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARM
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHH
RRAGLDYHEYAQMRVHRDWDAFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSE
HHHCCCHHHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCEEEECCC
TRGLPAELLERFPNEQRVRLPMRPDNRSLNLSNTVAVVVFEAWRQAGFEGGA
CCCCHHHHHHHCCCCCEEECCCCCCCCCEECCCEEEEEEEHHHHHCCCCCCC
>Mature Secondary Structure
MHARVAPLRPPQPNRNMFNVVLVAPEIPPNTGNVIRLCANTGAHLHLIEPLGFPLDDARM
CCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHH
RRAGLDYHEYAQMRVHRDWDAFVAAETPDPARMFAFTTRGSGRFHDHAFLSGDWFVFGSE
HHHCCCHHHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCCEEECCCEEEECCC
TRGLPAELLERFPNEQRVRLPMRPDNRSLNLSNTVAVVVFEAWRQAGFEGGA
CCCCHHHHHHHCCCCCEEECCCCCCCCCEECCCEEEEEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA