BacMap

Definition	Burkholderia cenocepacia HI2424 chromosome 1, complete sequence.
Accession	NC_008542
Length	3,483,902

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The map label for this gene is paaF [H]

Identifier: 116690738

GI number: 116690738

Start: 3010621

End: 3011388

Strand: Reverse

Name: paaF [H]

Synonym: Bcen2424_2718

Alternate gene names: 116690738

Gene position: 3011388-3010621 (Counterclockwise)

Preceding gene: 116690739

Following gene: 116690736

Centisome position: 86.44

GC content: 69.27

Gene sequence:

>768_bases
GTGGCCGACATTCAAGTGGAACGCGCCGACGGCGTGATGACGATCACGATCGCGCGCCCGGCGAAGAAGAATGCGCTGAC
GGCGGCGATGTACCAGACGATGGCCGATGCGCTCGCCGACGCGCAGGAAGACAAGGCGACGCGCGTGATCCTGCTGCGCG
GCAGCGACGGCAATTTCAGCGCGGGCAACGATCTCGAGGATTTCCTGAAGGCGCCGCCGCAGGACGATACCGCGTCGGTG
TTCCAGTTCCTGGCGCGGATCAGCAGCGCGAGCAAGCCGGTCGTGGCCGCGGTGCCGGGCATCGCGGTCGGCGTCGGCGT
GACGATGCTGCTGCACTGCGATCTGGTCTACGCGGCCGACACCGCGACGTTCTCGCTGCCGTTCGCGCAGCTCGGGTTGT
GCCCGGAAGCCGCGTCGAGCGCACTGCTGCCGCGGCTGGCCGGCCATCAGGTCGCCGCCGAGAAGCTGCTGCTGGGCGAG
GCGTTCGACGCGCTGGAAGCGCACCGGATCGGCATCGTCAACCGCGTGCTGCCGGCAGCCGAACTCGACGCGTTCGCGGC
GAAGCAGGCAGCGAAGCTGGCGGCGTTGCCGGCGGCATCGCTGCGGGTGACGAAGGCGCTGCTCAAGGATACGGGCGGCG
TGGCGACGCCCGCGCGGATGGCCGAGGAAGCGCGTCATTTCGCGACGATGCTGCGCGCGCCGGAAGCGCGCGAGGCGATG
ACCGCGTTCTTCGAGAAGCGCAAGCCGGATTTCCGTCAGTTCGACTGA

Upstream 100 bases:

>100_bases
CGCCTGTAAGCGGGACACGACGAGAAGCGACGACGGTGCGCGGGCCACGAGCTCGCGCACCGTTTTTTCATTCCGGTAGA
GACAGTTCAAGGAGACGGTT

Downstream 100 bases:

>100_bases
CGGACTGAGGCGCGCCGGCGGGAGACCGTCGGCGCACGCGCACGGTCTGGATCAGGCCGTGCCGTAATCGACGTGGCCGG
TTTCGCGGCAGAAGCTGACG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MADIQVERADGVMTITIARPAKKNALTAAMYQTMADALADAQEDKATRVILLRGSDGNFSAGNDLEDFLKAPPQDDTASV
FQFLARISSASKPVVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFAQLGLCPEAASSALLPRLAGHQVAAEKLLLGE
AFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATPARMAEEARHFATMLRAPEAREAM
TAFFEKRKPDFRQFD

Sequences:

>Translated_255_residues
MADIQVERADGVMTITIARPAKKNALTAAMYQTMADALADAQEDKATRVILLRGSDGNFSAGNDLEDFLKAPPQDDTASV
FQFLARISSASKPVVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFAQLGLCPEAASSALLPRLAGHQVAAEKLLLGE
AFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATPARMAEEARHFATMLRAPEAREAM
TAFFEKRKPDFRQFD
>Mature_254_residues
ADIQVERADGVMTITIARPAKKNALTAAMYQTMADALADAQEDKATRVILLRGSDGNFSAGNDLEDFLKAPPQDDTASVF
QFLARISSASKPVVAAVPGIAVGVGVTMLLHCDLVYAADTATFSLPFAQLGLCPEAASSALLPRLAGHQVAAEKLLLGEA
FDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATPARMAEEARHFATMLRAPEAREAMT
AFFEKRKPDFRQFD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=249, Percent_Identity=31.7269076305221, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI260275230, Length=249, Percent_Identity=31.7269076305221, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI260274832, Length=249, Percent_Identity=31.7269076305221, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=30.4, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=32.9411764705882, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=28.0155642023346, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=27.2373540856031, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=27.2373540856031, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757966, Length=215, Percent_Identity=30.2325581395349, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=215, Percent_Identity=30.2325581395349, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=23.7354085603113, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=23.7354085603113, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=23.7354085603113, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI68989263, Length=258, Percent_Identity=29.4573643410853, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=29.0322580645161, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=26.9767441860465, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI150378541, Length=247, Percent_Identity=29.1497975708502, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=27.9527559055118, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=27.807486631016, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=27.807486631016, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI62530384, Length=237, Percent_Identity=24.8945147679325, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=34.765625, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=33.206106870229, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=34, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=31.8007662835249, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=28.515625, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790281, Length=255, Percent_Identity=25.0980392156863, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=38.6046511627907, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=33.9920948616601, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=31.1475409836066, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=31.640625, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=31.9796954314721, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=204, Percent_Identity=27.9411764705882, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=178, Percent_Identity=29.2134831460674, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=24.896265560166, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=215, Percent_Identity=32.093023255814, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=215, Percent_Identity=32.093023255814, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=215, Percent_Identity=32.093023255814, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=184, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=26.9035532994924, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24654903, Length=250, Percent_Identity=30.8, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=30.2904564315353, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=30.2904564315353, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=33.206106870229, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=24.4094488188976, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=29.126213592233, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571730, Length=139, Percent_Identity=30.2158273381295, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571729, Length=139, Percent_Identity=30.2158273381295, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26976; Mature: 26845

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIQVERADGVMTITIARPAKKNALTAAMYQTMADALADAQEDKATRVILLRGSDGNFS
CCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGNDLEDFLKAPPQDDTASVFQFLARISSASKPVVAAVPGIAVGVGVTMLLHCDLVYAAD
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEC
TATFSLPFAQLGLCPEAASSALLPRLAGHQVAAEKLLLGEAFDALEAHRIGIVNRVLPAA
CCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATPARMAEEARHFATMLRAPEAREAM
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
TAFFEKRKPDFRQFD
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADIQVERADGVMTITIARPAKKNALTAAMYQTMADALADAQEDKATRVILLRGSDGNFS
CCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGNDLEDFLKAPPQDDTASVFQFLARISSASKPVVAAVPGIAVGVGVTMLLHCDLVYAAD
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEC
TATFSLPFAQLGLCPEAASSALLPRLAGHQVAAEKLLLGEAFDALEAHRIGIVNRVLPAA
CCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATPARMAEEARHFATMLRAPEAREAM
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
TAFFEKRKPDFRQFD
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]