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The map label for this gene is cls [H]

Identifier: 116690055

GI number: 116690055

Start: 2257102

End: 2258541

Strand: Direct

Name: cls [H]

Synonym: Bcen2424_2034

Alternate gene names: 116690055

Gene position: 2257102-2258541 (Clockwise)

Preceding gene: 116690054

Following gene: 116690057

Centisome position: 64.79

GC content: 66.67

Gene sequence:

>1440_bases
ATGACCCTCGACTGGCTACATCTCGGCACCCTGATCCTGACCATCCATGTGCTCGGGATCGTCGCGGCGTGCCACGCAAT
CATGAACACGCGCACGTCGCAAGGCGCGATCGCGTGGGCCGTCTCGCTCACGGCCATGCCGTACCTGACGCTCGTTCCAT
ACCTGTTCCTCGGCCGTAGCAAATTCTCCGGCTACGTCGACGCCCGGCGCCACGAGCTGGAAATGTTGCGCACACACACA
CCGCGTGCGCCGTGGCGCGCGACCGATGCGACCGACGGGCCGGCCGCCGACGCGCTGGGGCAGGCTGCCGTGCTCGCGCT
CACGCGGCTCGGCGGCATGCCGTTCCTCGGCGGCAATTCGGTACGCACGCTGGTCAACGGCGACGCGACATTCTCCGCCA
TCCTGTCGGCGATCGACGACGCGCGCGACTACGTGCTCGTCCAGTTCTTCATCGTGCGCGACGATGCGCTCGGCCGGATG
CTGCGCGACGCGCTGCTCGCGCGCGCGGCCGCCGGCGTACGCTGCTGCCTACTGTACGACAGCATCGGCAGCTTCGACCT
GCCGCACAGCTACGTCGACGCATTGCGCCGCGGTGGCGTCGAGGTCCATCCGTTCGCGACCAACCGGAAATTCGTCAATC
GCTTCCAGTTGAACTTCCGCAACCATCGCAAGATCGTCGTCGTCGACGGCAATCGCGCGTTCGTCGGCGGCCACAACGTC
GGTGTCGAATATCTCGGCGCGAAGCCGCGCCTGTCGCCATGGCGCGACACCCACATCGAGATCCGCGGCCCCGTGGTGGC
GAGCATCCAGTACGTGTTCGCCGAAGACTGGCACTGGGCGACGCAGAAACTGCCGCCCATCGCGCTGCCGCCGCCCGCGC
AGCCCGGCGACGGCATGCATTGCCTCGCGGTGCCGATGGGGCCGGCCGACAAGCAGGAAACCGGCTCGCTGTTCTTCGTC
GAATCGATCAATGCCGCCCGCGAGCGGGTCTGGATCACCACGCCGTACCTCGTTCCCGACGAAGCCGTGGTCTCCGCGCT
AAAACTCGCCGTGATGCGCGGCGTCGACGTACGCATCCTGATCCCGAGCCGGCGCGATCACTACGTCGTGTTCGAGGCGT
CCAAGCTTTATGCGCGCGACCTCGTCGATGCGGGCGTGAAAGTGTTCCGCTACCGGCCCGGCTTCCTGCACCAGAAGGTC
GTGCTGATCGACCGCATCGCGGCGGCGGTCGGCAGCGCGAATCTCGATAACCGTTCGTTCCGCCTCAATTTCGAGGTCAT
GGTCATGACCGTCGATCGCGCGTTCGCCGGCGAAGTCGAGACGATGCTCGATGCCGACTTCGCGCAGGCGTACGAGGTCG
ATGCGGACGAATACCGGCAGTCGCCTGCCTGGCGGCGCATCGCGATGCACGTCGCGCGGTTGTTCGCGCCGATCCTGTAA

Upstream 100 bases:

>100_bases
CGTTGCGCCGCACGGTTGGGTAAGATGCGCGTAGGCGGCCGCCCGGCCGCACGACTTCGGGAGTCCCACCGGGTGTCCCG
CAGGAGTCCTTTAAGCGCCC

Downstream 100 bases:

>100_bases
CGGCGGCGCACGCACGATCCGCACCGGCGCGGCGCACGCCGCGCATCGCCGTCACAGCAGCTTGTCGATGTCCGCTGCGA
TTTCATCGGGCTTGGTGGAC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLTAMPYLTLVPYLFLGRSKFSGYVDARRHELEMLRTHT
PRAPWRATDATDGPAADALGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILSAIDDARDYVLVQFFIVRDDALGRM
LRDALLARAAAGVRCCLLYDSIGSFDLPHSYVDALRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPIALPPPAQPGDGMHCLAVPMGPADKQETGSLFFV
ESINAARERVWITTPYLVPDEAVVSALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRIAAAVGSANLDNRSFRLNFEVMVMTVDRAFAGEVETMLDADFAQAYEVDADEYRQSPAWRRIAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLTAMPYLTLVPYLFLGRSKFSGYVDARRHELEMLRTHT
PRAPWRATDATDGPAADALGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILSAIDDARDYVLVQFFIVRDDALGRM
LRDALLARAAAGVRCCLLYDSIGSFDLPHSYVDALRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPIALPPPAQPGDGMHCLAVPMGPADKQETGSLFFV
ESINAARERVWITTPYLVPDEAVVSALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRIAAAVGSANLDNRSFRLNFEVMVMTVDRAFAGEVETMLDADFAQAYEVDADEYRQSPAWRRIAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLTAMPYLTLVPYLFLGRSKFSGYVDARRHELEMLRTHTP
RAPWRATDATDGPAADALGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILSAIDDARDYVLVQFFIVRDDALGRML
RDALLARAAAGVRCCLLYDSIGSFDLPHSYVDALRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNVG
VEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPIALPPPAQPGDGMHCLAVPMGPADKQETGSLFFVE
SINAARERVWITTPYLVPDEAVVSALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVV
LIDRIAAAVGSANLDNRSFRLNFEVMVMTVDRAFAGEVETMLDADFAQAYEVDADEYRQSPAWRRIAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=473, Percent_Identity=27.906976744186, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1787007, Length=342, Percent_Identity=31.8713450292398, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53113; Mature: 52982

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLTAMPYLTLVPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHELEMLRTHTPRAPWRATDATDGPAADALGQAAVLALTRLGGMPFLGGNS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFSAILSAIDDARDYVLVQFFIVRDDALGRMLRDALLARAAAGVRCCLLYD
EEEEECCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHSYVDALRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHEECCCCCCEEEEEECCEEEECCCCC
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPIALPPPAQPGDGMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
CLAVPMGPADKQETGSLFFVESINAARERVWITTPYLVPDEAVVSALKLAVMRGVDVRIL
EEEECCCCCCCCCCCCEEEEECCCCCHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRIAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEVMVMTVDRAFAGEVETMLDADFAQAYEVDADEYRQSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECCHHHCCHHHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLTAMPYLTLVPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHELEMLRTHTPRAPWRATDATDGPAADALGQAAVLALTRLGGMPFLGGNS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFSAILSAIDDARDYVLVQFFIVRDDALGRMLRDALLARAAAGVRCCLLYD
EEEEECCCHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHSYVDALRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHEECCCCCCEEEEEECCEEEECCCCC
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPIALPPPAQPGDGMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCE
CLAVPMGPADKQETGSLFFVESINAARERVWITTPYLVPDEAVVSALKLAVMRGVDVRIL
EEEECCCCCCCCCCCCEEEEECCCCCHHEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRIAAAVGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEVMVMTVDRAFAGEVETMLDADFAQAYEVDADEYRQSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECCHHHCCHHHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA