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The map label for this gene is pucI [H]

Identifier: 116689986

GI number: 116689986

Start: 2183186

End: 2184670

Strand: Reverse

Name: pucI [H]

Synonym: Bcen2424_1965

Alternate gene names: 116689986

Gene position: 2184670-2183186 (Counterclockwise)

Preceding gene: 116689987

Following gene: 116689985

Centisome position: 62.71

GC content: 64.92

Gene sequence:

>1485_bases
ATGGCTCAGTTCAGTGTGGCGCACGACAGCGCCTATCCGGCGGAAGAAGGCGGCGAGGGCGGCGGTCCGGCACTGCCGGA
TGGTTACAGCGAACGTCTGTACAACGAAGATTTGGCACCCCTTAAAAATCAGACATGGGGTTCGTACAACATCTTCGCGT
TCTGGATGTCGGACGTGCACAGCGTCGGCGGCTACGTGTTCGCGGGCAGTCTGTTTGCGCTCGGGCTGACGAGCTGGCAA
GTGCTGATCGCGCTGATCGTCGGCATCGGCCTCGTGAACGCGCTGTGCAACCTGATCGCGCGGCCGAGCCAGCAGATCGG
CGTGCCGTACCCGGTCGCGTGCCGCGCGACCTTCGGCGTGCTCGGCGCGAACGTGCCGGCCGTGATCCGCGGGCTGATCG
CGATCGCGTGGTACGGCATCCAGACTTATCTCGCGTCGAGCGCGCTCGTGATCGTCGTGCTGAAATTCTTCCCGCAATGG
CTGCCGTATGCGGACGTCCATCGCTACGGCTTCCTCGGGCTGTCGGCGGTCGGCTGGGCCGGCTTCATGCTGCTGTGGGT
GCTCCAGGCGTTCGTGTTCTGGAACGGCATGGAGACGATCAAGAAGTTCATCGACTTCGCGGGCCCGGCCGTCTATGTCG
TGATGTTCATCCTGGCCGGTTACATGGTGTGGCGCGCGGGCTGGCGCAACATCGGCATCAACCTCGGCGGCGTCAAGTAT
TACGGCGCGGAAGTGATTCCGGTGATGATCACCGCAGTGTCGCTCGTCGTGTCGTACTTCTCCGGGCCGATGCTGAACTT
CGGCGATTTCTCGCGCTACTGCCGCTCGTTCGGCGACGTGAAGCGCGGCAATTTCTGGGGGCTGCCGGTCAACTTTCTCG
CGTTCTCGCTCGTCACGGTGATCACCACCGCCGCGACGCTGCCGGTGTTCGGCGAACTGATCACCGACCCGGTCGCGACG
GTCGGCCGCATCGACCATCCGACCGCGGTGATCCTCGGCGCGCTGACGTTCACGATCGCGACGATCGGCATCAACATCGT
CGCGAACTTCGTATCGCCCGCGTTCGATTTCTCGAACGTCGCGCCGCGCCTGATCAGCTGGCGCGCAGGCGGGATGCTCG
CCGCCGTCGCGTCGATCTTCATCACGCCGTGGAACCTGTTCAACAACCCGGCCGTGATCCACTACACGCTCGACGTGCTC
GGCGCGTTCATCGGCCCGCTGTACGGCGTGCTGATCGCCGATTTCTACCTCGTCAAGCGTGGCCGCCTCGTGCGCGACGA
CCTGTACACGATGTCGGAAAGCGGCGCCTATTGGTACACGGGCGGCGTGAACCGCCGCGCGCTCGCCGCGCTGCTGCCGG
CCGCGGTGATCGCGGTGGTCTGCGTGATGGTGCCGGGCTGGCAGGCCCTCGCGAATTTCTCGTGGTTCATCGGCGCGGGG
CTCGGCTTCGTGTTCTACCGGCTGCTCGCGAGCGCGAAGGCGTAA

Upstream 100 bases:

>100_bases
CTGCGGTGTTTGCCGCGGGCCGCTGGTCCGACTCCGGGCGATGATCCGGAGCGATATCGATGACGGTGGAGCGGCCTGGC
GGCCGCGTCAAGGAGAAGTC

Downstream 100 bases:

>100_bases
GGAGCATGCGATGAAGATCCGACTGATCAATCCGAATACGACGCAGCGGATGACCGACGCGATGGGCCGCTGCGCGCGCG
AGGTCGCGGCCGCCGGCACC

Product: permease for cytosine/purines, uracil, thiamine, allantoin

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQFSVAHDSAYPAEEGGEGGGPALPDGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGIGLVNALCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
YGAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGDVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVAT
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGAYWYTGGVNRRALAALLPAAVIAVVCVMVPGWQALANFSWFIGAG
LGFVFYRLLASAKA

Sequences:

>Translated_494_residues
MAQFSVAHDSAYPAEEGGEGGGPALPDGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGIGLVNALCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
YGAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGDVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVAT
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGAYWYTGGVNRRALAALLPAAVIAVVCVMVPGWQALANFSWFIGAG
LGFVFYRLLASAKA
>Mature_493_residues
AQFSVAHDSAYPAEEGGEGGGPALPDGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQV
LIALIVGIGLVNALCNLIARPSQQIGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQWL
PYADVHRYGFLGLSAVGWAGFMLLWVLQAFVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKYY
GAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGDVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVATV
GRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVLG
AFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGAYWYTGGVNRRALAALLPAAVIAVVCVMVPGWQALANFSWFIGAGL
GFVFYRLLASAKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=471, Percent_Identity=29.0870488322718, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=482, Percent_Identity=25.7261410788382, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322219, Length=439, Percent_Identity=23.9179954441913, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319429, Length=467, Percent_Identity=23.1263383297645, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323266, Length=470, Percent_Identity=22.9787234042553, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324766, Length=468, Percent_Identity=22.008547008547, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=20.9401709401709, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53481; Mature: 53350

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCC
>Mature Secondary Structure 
AQFSVAHDSAYPAEEGGEGGGPALPDGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVH
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHH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HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]