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The map label for this gene is pleD [H]

Identifier: 116689924

GI number: 116689924

Start: 2108917

End: 2110317

Strand: Reverse

Name: pleD [H]

Synonym: Bcen2424_1903

Alternate gene names: 116689924

Gene position: 2110317-2108917 (Counterclockwise)

Preceding gene: 116689925

Following gene: 116689922

Centisome position: 60.57

GC content: 65.88

Gene sequence:

>1401_bases
ATGAGTACGACCTATGCAGCCGGGGAACCGCCGCGCGTCGTCAGCGCGGCCGCGAGGCTCTACGCGAAATGCCTGCTGGT
CGGATTCGCGTTGCTGCCCGCCTATCTGATTGCCTATCTGTGGTTCTTCAGCGACCGGGGCGTCGTGTTCGAGAACCACG
CTTTCCATGAAATCGCGATCGCGGCGGCGACACTCGAAGGCGCGTTCGTCACCTACGTGACGTGGGTGTGCTACCGGTCG
TCAGGCGAGCCGCTGCTGCGCTGGCTGACGCTCGGTTTTCTCGGCTTCGCGATGATCTATGCGCTGCACGGGGCGTTCAC
CGGGATGGCGCACCACAATATCTGGCTGTTCCTGCTGTACGGGCCCGCGTCGCGGTTCGTGATGTCGGCGCTGCTGCTCA
CGGGCCTGCTGTCGTATTCGCGTCCGCCGGACCGGCTCGACAAGCGCACGAGCATGCGGACGTGGTTGCCGTGGATCGTG
TTCTTCCTGATCGTCAACGTGGCGGTTGCGTACATCGCCTATTCGCCGGTCGCGGGCGCGCTGGGCACGCGGCTGTCGAT
GGAAGGCGGCGCGCTGGTGTTCTCGCTGCTGAACGTGTGCCTGCTGCTCGCGCGGCGCATCCGCTCGCCGCTGATGGTGA
TCTACGGCGTGTCGATCATGGCGTTCGCGCTATCGTCGCTCGCGTTCATCCTCGGCAAGCCGTGGAATCACATGTGGTGG
CTCGCGCATGCGATCTTCGCGGGCGGATTCTTCCTGCTCAGTTACGGCGTCGTGCAGGCGCTGCAGACGACACGCTCGTT
CTCGGCGATCTACAGCCAGGAAGACCTGATGAGCCGGCTGTCGGAATCGATGGCGCGTACCGAGAGCGCGCTGCAGGAAC
TGCGGCGCACGAACCAGAAGCTCGAATACATGGCCGCGACCGACCCGATGACGGGCGCGTCGAACCGCCGGCAGTTCATC
GCGCAGGTCGAGCGTGAGATCGTGCGGGCCGAGCGAGACGGCACGCCGTTCTGCCTGCTCGCGCTCGATCTCGACAATTT
CAAGAACATCAACGATGCATACGGGCACCAGATCGGCGACGAGGTGCTGCGCGGCGTCGTGCGCCAGTGCCTCGAGGCAA
TTCGGCCGGCGGGCGCGCTCGCGCGGGTCGGCGGCGAGGAGTTCATGGCGCTCTTGCCGGAGATGCCGCTCGAAGGCGCG
CGGATGACCGCCGAGCGCGTGCGTTCGTCGATTTCGAGTTCGCCGTTCGGGCTCGACTTCAAGCGCGTGCAGGTGACGGT
CAGCGTCGGCGTCGCGCAATACGGCGTCGACGGCAGCACGGTCGATGCGCTGCTGCGGGTGGTCGACGAGCGGCTGTATC
AGGCGAAGCGGGAAGGGCGCAACCGCGTGGTGTCGCGCTAA

Upstream 100 bases:

>100_bases
TGCTCTGACATTTGCCTTTCGGCACGATGGGCCAGATCGCGCGTCGCGTGATCCGTGCCGAGACGATTCGAACCAACGCA
GCAGACCGACCGGGGACCGA

Downstream 100 bases:

>100_bases
CCGGATCTGCACCGAAAGCCCCGGCGGCGGGAGTGCCGCCGGGCTTTTGCGGGCCGGTGTCGGTGTCGGCCTGCTCACGC
GCGGCGACGCGCTAGCGCGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSTTYAAGEPPRVVSAAARLYAKCLLVGFALLPAYLIAYLWFFSDRGVVFENHAFHEIAIAAATLEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRFVMSALLLTGLLSYSRPPDRLDKRTSMRTWLPWIV
FFLIVNVAVAYIAYSPVAGALGTRLSMEGGALVFSLLNVCLLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREIVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLEAIRPAGALARVGGEEFMALLPEMPLEGA
RMTAERVRSSISSSPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVSR

Sequences:

>Translated_466_residues
MSTTYAAGEPPRVVSAAARLYAKCLLVGFALLPAYLIAYLWFFSDRGVVFENHAFHEIAIAAATLEGAFVTYVTWVCYRS
SGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRFVMSALLLTGLLSYSRPPDRLDKRTSMRTWLPWIV
FFLIVNVAVAYIAYSPVAGALGTRLSMEGGALVFSLLNVCLLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFI
AQVEREIVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLEAIRPAGALARVGGEEFMALLPEMPLEGA
RMTAERVRSSISSSPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVSR
>Mature_465_residues
STTYAAGEPPRVVSAAARLYAKCLLVGFALLPAYLIAYLWFFSDRGVVFENHAFHEIAIAAATLEGAFVTYVTWVCYRSS
GEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLYGPASRFVMSALLLTGLLSYSRPPDRLDKRTSMRTWLPWIVF
FLIVNVAVAYIAYSPVAGALGTRLSMEGGALVFSLLNVCLLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWWL
AHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQKLEYMAATDPMTGASNRRQFIA
QVEREIVRAERDGTPFCLLALDLDNFKNINDAYGHQIGDEVLRGVVRQCLEAIRPAGALARVGGEEFMALLPEMPLEGAR
MTAERVRSSISSSPFGLDFKRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVSR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=36.3095238095238, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=41.4772727272727, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI145693134, Length=206, Percent_Identity=32.0388349514563, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788085, Length=182, Percent_Identity=31.8681318681319, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=129, Percent_Identity=38.7596899224806, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=31.3953488372093, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788956, Length=260, Percent_Identity=27.6923076923077, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=29.2993630573248, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081977, Length=177, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51780; Mature: 51649

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTYAAGEPPRVVSAAARLYAKCLLVGFALLPAYLIAYLWFFSDRGVVFENHAFHEIAI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHH
AAATLEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRFVMSALLLTGLLSYSRPPDRLDKRTSMRTWLPWIVFFLIVNVAVAYIAYSPVAGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLSMEGGALVFSLLNVCLLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEYMAATDPMTGASNRRQFIAQVEREIVRAERDGTPFCLLALDLDNFKNINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHH
EVLRGVVRQCLEAIRPAGALARVGGEEFMALLPEMPLEGARMTAERVRSSISSSPFGLDF
HHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVSR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STTYAAGEPPRVVSAAARLYAKCLLVGFALLPAYLIAYLWFFSDRGVVFENHAFHEIAI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHH
AAATLEGAFVTYVTWVCYRSSGEPLLRWLTLGFLGFAMIYALHGAFTGMAHHNIWLFLLY
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GPASRFVMSALLLTGLLSYSRPPDRLDKRTSMRTWLPWIVFFLIVNVAVAYIAYSPVAGA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTRLSMEGGALVFSLLNVCLLLARRIRSPLMVIYGVSIMAFALSSLAFILGKPWNHMWW
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHAIFAGGFFLLSYGVVQALQTTRSFSAIYSQEDLMSRLSESMARTESALQELRRTNQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEYMAATDPMTGASNRRQFIAQVEREIVRAERDGTPFCLLALDLDNFKNINDAYGHQIGD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHH
EVLRGVVRQCLEAIRPAGALARVGGEEFMALLPEMPLEGARMTAERVRSSISSSPFGLDF
HHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
KRVQVTVSVGVAQYGVDGSTVDALLRVVDERLYQAKREGRNRVVSR
EEEEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA