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The map label for this gene is lipA [H]

Identifier: 116689857

GI number: 116689857

Start: 2039967

End: 2040932

Strand: Reverse

Name: lipA [H]

Synonym: Bcen2424_1836

Alternate gene names: 116689857

Gene position: 2040932-2039967 (Counterclockwise)

Preceding gene: 116689858

Following gene: 116689848

Centisome position: 58.58

GC content: 72.67

Gene sequence:

>966_bases
ATGACCGCCGCGCTCGAACGACCCAGCCGCACGGCGCTCGGCCAGCCCGGCGCGAGAAGTCGCGACAAGCTCGCGCGCAT
TCCGGTGCGCGTCGAGCCCGCGGCCGGCGCGGCGCTGCCGAAGCCGCCGTGGCTGCGCGCGCGGCCGATGATGAGCGCGG
CGGTGGCCGACATGGCCGCCGTGCTGCGCGAGCATCGGCTGCATTCCGTCTGCGAGGAGGCGATGTGTCCGAACATCGGC
GAATGCTTCGCGCAGCGCACCGCGACCTTCATGATCATGGGCGGACTGTGTACGCGGCGTTGCCCGTTCTGCGATGTCGC
GCACGGCCGCCCCGAGCCGCTCGATCCCGACGAGCCCGCGCGGCTGGCCGACGCGGCGGCGGCGCTCGGGTTGCGCTACG
TCGTGATCACGTCGGTCGACCGCGACGACCTGCGCGACGGCGGCGCCGCGCATTTCGCCGCGTGCATCGCGGCGGTGCGC
GCGAGCGTGCCGGGTATCGGTGTCGAGGTGCTGACGCCGGACTTCCGCGGCCGTGTGGCGCGTGCGCTCGACGCGCTGTC
GTCGGCGTGGCCCGATGTGTTCAACCACAACATCGAGACGGTGCCGTCGCTGTATCGCGCGGCGCGGCCGGGCGCCGACT
ATCGCGGCTCGCTCGAACTGCTCGCGCAGGCGAAGGCCGCGCGGCCGACGCTCGTGACGAAATCGGGCTTGATGCTCGGG
CTCGGCGAGCGCGACGACGAGGTGCGCGACACGCTGCGCGACCTGCGCGCGCACGAGGTCGACGTGCTGACGCTCGGCCA
GTATCTGGCGCCGTCGGAACACCATTTGCCGGTGCGGCGTTACGTGAGCCCGGAGGCTTTCGCCGCATGGCACGACGAGG
CGCTCGCGCTCGGTTTCAGGGAAGTCGTCGCCGGCCCGCTCGTGCGGTCGTCGTATCACGCGGCCGACGTGCTGGAGAGC
GCGTAG

Upstream 100 bases:

>100_bases
GCGTGCGTGCCGAGGTGATCGCCGGCAGCGGGCACATGGTGCAGATGGAAGCGGCCGCCGACGTGAACCGCCTGATCGTC
GCGTTCCTCGGAGACTGACG

Downstream 100 bases:

>100_bases
CGCGCCGCGCGTCAGGCCAAGGCGGCGTGCGCGCGACTGATGCGGCGCAGGTACAGCACGAGCCCCGCGCCCGCGAGCAT
CAGGCTGACCGTGTTCGCGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTAALERPSRTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLREHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLTPDFRGRVARALDALSSAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKAARPTLVTKSGLMLG
LGERDDEVRDTLRDLRAHEVDVLTLGQYLAPSEHHLPVRRYVSPEAFAAWHDEALALGFREVVAGPLVRSSYHAADVLES
A

Sequences:

>Translated_321_residues
MTAALERPSRTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLREHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLTPDFRGRVARALDALSSAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKAARPTLVTKSGLMLG
LGERDDEVRDTLRDLRAHEVDVLTLGQYLAPSEHHLPVRRYVSPEAFAAWHDEALALGFREVVAGPLVRSSYHAADVLES
A
>Mature_320_residues
TAALERPSRTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLREHRLHSVCEEAMCPNIGE
CFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRA
SVPGIGVEVLTPDFRGRVARALDALSSAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKAARPTLVTKSGLMLGL
GERDDEVRDTLRDLRAHEVDVLTLGQYLAPSEHHLPVRRYVSPEAFAAWHDEALALGFREVVAGPLVRSSYHAADVLESA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=46.8965517241379, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=47.4103585657371, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786846, Length=298, Percent_Identity=59.3959731543624, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=45.2631578947368, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=284, Percent_Identity=44.7183098591549, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI221513272, Length=297, Percent_Identity=46.4646464646465, Blast_Score=267, Evalue=6e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34615; Mature: 34484

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAALERPSRTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLREHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHH
RLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLTPDFRGRVA
HHHHHHHHHHEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
RALDALSSAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKAARPTLVTKSGLMLG
HHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEE
LGERDDEVRDTLRDLRAHEVDVLTLGQYLAPSEHHLPVRRYVSPEAFAAWHDEALALGFR
CCCCCHHHHHHHHHHHHHCCHHEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
EVVAGPLVRSSYHAADVLESA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAALERPSRTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLREHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCHH
RLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLTPDFRGRVA
HHHHHHHHHHEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
RALDALSSAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKAARPTLVTKSGLMLG
HHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCEEE
LGERDDEVRDTLRDLRAHEVDVLTLGQYLAPSEHHLPVRRYVSPEAFAAWHDEALALGFR
CCCCCHHHHHHHHHHHHHCCHHEEHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
EVVAGPLVRSSYHAADVLESA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA