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The map label for this gene is mpl [H]

Identifier: 116688633

GI number: 116688633

Start: 680405

End: 681790

Strand: Direct

Name: mpl [H]

Synonym: Bcen2424_0610

Alternate gene names: 116688633

Gene position: 680405-681790 (Clockwise)

Preceding gene: 116688631

Following gene: 116688634

Centisome position: 19.53

GC content: 68.61

Gene sequence:

>1386_bases
ATGCACATCCACATTCTTGGCATCTGCGGCACCTTCATGGGCGGTCTCGCCGTACTCGCACGCGAGGCGGGCCACACGGT
GACGGGTTGCGACGCGGGCGTCTATCCGCCGATGAGCACGCAGCTCGAGGCGCAGGGCATCACGCTGATCGAGGGCTACG
GCGCCGAGCAGGTCGACCTGAAGCCGGACCTGTTCGTGATCGGCAACGTCGTCACGCGCGGCAATCCGCTGATGGAGGCG
ATTCTCGATCGCGGCCTGCCTTACGTGTCGGGCCCGCAGTGGCTCGGCGAGCACGTGCTGGCCGGCAAATGGGTGCTCGC
GGTCGCGGGCACGCACGGCAAGACGACCACATCGTCGATGCTCGCGTGGCTGCTGGAAGACGCAGGTCTCAATCCGGGCT
TCCTGATCGGCGGCGTGCCGCTGAACTTCGGCGTGTCCGCGCGGCTCACCGATTCGAGCTTCTTCGTGATCGAGGCCGAC
GAGTACGACACGGCATTCTTCGACAAGCGCTCGAAGTTCGTGCACTACCGGCTGCGCACCGCGGTGCTGAACAACCTCGA
ATTCGATCACGCCGACATCTTCCCCGATCTTGCCGCGATCGAAACGCAATTCCATCATCTGGTGCGCACCGTGCCCGGCG
TCGGGCGGATCGTCACGAACGGCCGCTCGGACGCCCTCGAGCGCGTGCTGGCGCGCGGCTGCTGGAGCGAGGTCGAACGC
TTCGGCGTCGACGGCGGCTGGCAGGCGCTGCCGGCCGAGGACGGCGTGCCGGTCGACGAACGCTTCGCGGTGTATTCGCA
TGCCGGGCGCGTCGGCGAAGTCGCATGGCAGGTGCAGGGCGACCACAACCGGATGAACGCGCTCGCGGCGATCGCCGCCG
CGCGCCACGTCGGCGTGCCGCCAGCGCAGGCGGCCGAATCGCTCGCCTCGTTCCGCAACGTGAAGCGCCGCATGGAAGTG
CGCGGCAGCGTGGACGGCGTGACCGTCTATGACGACTTCGCGCACCATCCGACCGCGATCGACACCACGATCGCCGGCCT
TCGTGCGCGCATCGGCCGCCAGAATGCCCGCATCCTCGCCGTGCTCGAGCCGCGCTCGAACACGATGAAGCTCGGCGTGA
TGAAGTCGCAGTTGCCGGCGAGCCTCGCCGATGCCGATCTCGTGTTCGGCTACGGCGCGCCGACCGGGCGCGATGCGCTC
GGCTGGAATCTCGCCGAAGCGCTCGCGCCGCTCGGCGACCGCGCACGCGCCTTCGACGATCTGCATCTGCTGGTGAAGGC
GGTGGTCGAGGCCGCGCGTCCGGGCGATCACGTGCTCGTGATGAGCAACGGCGGCTTCGGCGGCGTGCACCAGAAGCTGC
TCGATGCGCTCGGGAGCCGCCCGTGA

Upstream 100 bases:

>100_bases
CAAAGTCGTGGGCCGGACGCCGGCCGCACGGCCCGTCCGGCGCGACACACACGCATCCTTTCCGAATCGACGGCGCCCGG
CGGCGCGAAAGCATTCCTCT

Downstream 100 bases:

>100_bases
TCCTGTATCTGCACGGCTTCCGGTCGTCGCCGGAATCGCAGAAGTCGCGACTGCTCGCCGCGCGCATGGCCGAACTCGGC
CGCACCGGCGAGTGGCGGTG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQVDLKPDLFVIGNVVTRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRLRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVER
FGVDGGWQALPAEDGVPVDERFAVYSHAGRVGEVAWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIDTTIAGLRARIGRQNARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDAL
GWNLAEALAPLGDRARAFDDLHLLVKAVVEAARPGDHVLVMSNGGFGGVHQKLLDALGSRP

Sequences:

>Translated_461_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQVDLKPDLFVIGNVVTRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRLRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVER
FGVDGGWQALPAEDGVPVDERFAVYSHAGRVGEVAWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIDTTIAGLRARIGRQNARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDAL
GWNLAEALAPLGDRARAFDDLHLLVKAVVEAARPGDHVLVMSNGGFGGVHQKLLDALGSRP
>Mature_461_residues
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQVDLKPDLFVIGNVVTRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRLRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVER
FGVDGGWQALPAEDGVPVDERFAVYSHAGRVGEVAWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIDTTIAGLRARIGRQNARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDAL
GWNLAEALAPLGDRARAFDDLHLLVKAVVEAARPGDHVLVMSNGGFGGVHQKLLDALGSRP

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=460, Percent_Identity=59.7826086956522, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1786279, Length=362, Percent_Identity=28.1767955801105, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49438; Mature: 49438

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQVDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECC
KPDLFVIGNVVTRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRLRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVER
HHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
FGVDGGWQALPAEDGVPVDERFAVYSHAGRVGEVAWQVQGDHNRMNALAAIAAARHVGVP
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
PAQAAESLASFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIDTTIAGLRARIGRQNARILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLGDRARAFDD
EECCCCCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHH
LHLLVKAVVEAARPGDHVLVMSNGGFGGVHQKLLDALGSRP
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQVDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECC
KPDLFVIGNVVTRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRLRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
AVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVER
HHHHCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
FGVDGGWQALPAEDGVPVDERFAVYSHAGRVGEVAWQVQGDHNRMNALAAIAAARHVGVP
HCCCCCCEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCC
PAQAAESLASFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIDTTIAGLRARIGRQNARILA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLGDRARAFDD
EECCCCCCEEEEEEHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHH
LHLLVKAVVEAARPGDHVLVMSNGGFGGVHQKLLDALGSRP
HHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]