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The map label for this gene is pcaF [H]

Identifier: 116688566

GI number: 116688566

Start: 601961

End: 603163

Strand: Reverse

Name: pcaF [H]

Synonym: Bcen2424_0542

Alternate gene names: 116688566

Gene position: 603163-601961 (Counterclockwise)

Preceding gene: 116688567

Following gene: 116688565

Centisome position: 17.31

GC content: 68.58

Gene sequence:

>1203_bases
ATGACAGACGCCTACATCTGCGATGCGATTCGCACCCCCATCGGCCGCTACGGCGGCGCCCTGAAAGATGTTCGTGCCGA
CGACCTCGGCGCAGTGCCGCTCAAGGCGCTGATCGAGCGCAATCGCGACGTCGACTGGGCGGCGATCGACGACGTGATCT
ACGGCTGCGCGAATCAGGCCGGCGAGGACAACCGCAACGTCGCGCGCATGTCGGCGCTGCTGGCGGGCCTGCCTACTGCC
GTGCCGGGCACGACGCTGAACCGGCTGTGCGGTTCCGGGATGGACGCGGTCGGCACGGCCGCGCGCGCGATCAAGGCAGG
TGAAGCGCGCCTGATGATTGCAGGCGGCGTCGAGAGCATGACGCGCGCGCCGTTCGTGATGGGCAAGGCCGCAAGCGCGT
TCGCGCGCCAGGCCGACATCTACGACACGACGATCGGCTGGCGCTTCGTCAATCCGCTGATGAAGCAGCTGTACGGTGTC
GATTCGATGCCGGAAACCGCCGAGAACGTCGCGGTCGACTACGACATCAGCCGCGCGGATCAGGACCTGTTCGCGCTGCG
CAGCCAGCAGAAGGCCGCGCGGGCGCAGCAGGACGGCACGCTCGCTGAAGAAATCGTCGCGGTCACGATTCCGCAGAAGA
AAGGCGATGCCATCGTTGTGTCGCGCGACGAACATCCGCGCGAAACGTCGCTCGAAGCGCTCGCGAAACTGAAGGGCGTC
GTGCGCCCGGACGGCTCGGTCACGGCCGGCAACGCGTCGGGCGTGAACGACGGCGCCTGCGCGCTGCTGCTCGCCAATGC
GCAGGCTGCCGACCAGTATGGCCTGCGCCGCCGCGCGCGCGTCGTCGGCATGGCGACCGCCGGCGTCGAGCCGCGCGTGA
TGGGCATCGGCCCCGCGCCGGCCACGCAGAAACTGCTGCGCCAGCTCGACATGCGCATCGACCAGTTCGACGTGATCGAA
CTGAACGAGGCATTCGCGTCGCAAGGTCTCGCCGTGCTGCGCATGCTCGGCGTGGCCGACGATGATCCGCGCGTGAACCC
GAATGGCGGTGCGATCGCGCTGGGCCACCCGCTCGGCGCATCGGGCGCGCGGCTCGTCACCACGGCACTCCATCAACTCG
AGCGTACCGGCGGCCGCTTCGCGCTCTGTACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATCGCGATCGAACGCGTG
TAA

Upstream 100 bases:

>100_bases
CCAGACGGCATAACCCGATTGACCGACGTGCAAACGCCACTACGTTCTCTACCATGAACGAATCCGCCACCCGGCAGGTT
CGTCACTCACCGATACGCTC

Downstream 100 bases:

>100_bases
CCCACGCGCCGCCCGCCTATAACGAAGTCATGAAGGAGACACTGCATGTCCTATCAGGCGATCCAGCTGGATATCGATCA
GGCCGCGGGCGTGGCCACGG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALIERNRDVDWAAIDDVIYGCANQAGEDNRNVARMSALLAGLPTA
VPGTTLNRLCGSGMDAVGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLYGV
DSMPETAENVAVDYDISRADQDLFALRSQQKAARAQQDGTLAEEIVAVTIPQKKGDAIVVSRDEHPRETSLEALAKLKGV
VRPDGSVTAGNASGVNDGACALLLANAQAADQYGLRRRARVVGMATAGVEPRVMGIGPAPATQKLLRQLDMRIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTALHQLERTGGRFALCTMCIGVGQGIAIAIERV

Sequences:

>Translated_400_residues
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALIERNRDVDWAAIDDVIYGCANQAGEDNRNVARMSALLAGLPTA
VPGTTLNRLCGSGMDAVGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLYGV
DSMPETAENVAVDYDISRADQDLFALRSQQKAARAQQDGTLAEEIVAVTIPQKKGDAIVVSRDEHPRETSLEALAKLKGV
VRPDGSVTAGNASGVNDGACALLLANAQAADQYGLRRRARVVGMATAGVEPRVMGIGPAPATQKLLRQLDMRIDQFDVIE
LNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTALHQLERTGGRFALCTMCIGVGQGIAIAIERV
>Mature_399_residues
TDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALIERNRDVDWAAIDDVIYGCANQAGEDNRNVARMSALLAGLPTAV
PGTTLNRLCGSGMDAVGTAARAIKAGEARLMIAGGVESMTRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLYGVD
SMPETAENVAVDYDISRADQDLFALRSQQKAARAQQDGTLAEEIVAVTIPQKKGDAIVVSRDEHPRETSLEALAKLKGVV
RPDGSVTAGNASGVNDGACALLLANAQAADQYGLRRRARVVGMATAGVEPRVMGIGPAPATQKLLRQLDMRIDQFDVIEL
NEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGASGARLVTTALHQLERTGGRFALCTMCIGVGQGIAIAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=40.7594936708861, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.7909319899244, Blast_Score=255, Evalue=6e-68,
Organism=Homo sapiens, GI4557237, Length=407, Percent_Identity=34.1523341523341, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=39.3120393120393, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.9133489461358, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=29.8994974874372, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.3291770573566, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.6575682382134, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.7160493827161, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=43.75, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1788683, Length=414, Percent_Identity=31.8840579710145, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=43.0348258706468, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=33.1683168316832, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI17535921, Length=410, Percent_Identity=33.4146341463415, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=31.6279069767442, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.8765432098765, Blast_Score=151, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=36.4532019704434, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=414, Percent_Identity=36.231884057971, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.3915211970075, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=38.1773399014778, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=35.9102244389027, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=30.1176470588235, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42106; Mature: 41974

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALIERNRDVDWAAIDDVIYGCANQA
CCCHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTAVPGTTLNRLCGSGMDAVGTAARAIKAGEARLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLYGVDSMPETAENVAVDYDISRAD
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEHHHHH
QDLFALRSQQKAARAQQDGTLAEEIVAVTIPQKKGDAIVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGSVTAGNASGVNDGACALLLANAQAADQYGLRRRARVVGMATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCH
ATQKLLRQLDMRIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTALHQLERTGGRFALCTMCIGVGQGIAIAIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TDAYICDAIRTPIGRYGGALKDVRADDLGAVPLKALIERNRDVDWAAIDDVIYGCANQA
CCHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPTAVPGTTLNRLCGSGMDAVGTAARAIKAGEARLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
TRAPFVMGKAASAFARQADIYDTTIGWRFVNPLMKQLYGVDSMPETAENVAVDYDISRAD
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEHHHHH
QDLFALRSQQKAARAQQDGTLAEEIVAVTIPQKKGDAIVVSRDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGSVTAGNASGVNDGACALLLANAQAADQYGLRRRARVVGMATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCH
ATQKLLRQLDMRIDQFDVIELNEAFASQGLAVLRMLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTALHQLERTGGRFALCTMCIGVGQGIAIAIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]