Definition Burkholderia cenocepacia HI2424 chromosome 1, complete sequence.
Accession NC_008542
Length 3,483,902

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The map label for this gene is paaG [H]

Identifier: 116688565

GI number: 116688565

Start: 601124

End: 601915

Strand: Reverse

Name: paaG [H]

Synonym: Bcen2424_0541

Alternate gene names: 116688565

Gene position: 601915-601124 (Counterclockwise)

Preceding gene: 116688566

Following gene: 116688564

Centisome position: 17.28

GC content: 67.3

Gene sequence:

>792_bases
ATGTCCTATCAGGCGATCCAGCTGGATATCGATCAGGCCGCGGGCGTGGCCACGGTCACGCTCAACCGCCCCGACAAGCT
GAACAGCTTCACGCGGGTGATGCATCGCGAACTGCAGTCGGCGCTCGATGAGGTCGAGGTGGCCGGCGCGCGCGCGCTGA
TCCTGACGGGTGCGGGGCGCGGCTTTTGCGCGGGTCAGGATCTGGCCGACCTCGACTTCACGCCGGGCGCGTCCACCGAT
CTCGGCACGCTGATCGACGAGCATTTCAATCCACTGATCCGCCGCCTGCAGCGTCTGCCGATTCCGGTGATCGCCGCCGT
CAACGGCACGGCAGCCGGCGCCGGCGCGAATCTGGCACTGGCGTGCGACCTGGTGTTTGCCGCCCGCTCGAGCAGTTTCA
TCCAGGCCTTCATCAAGATCGGACTCGTGCCCGATTCGGGCGGCACGTGGTTCCTGCCGCAACGCGTCGGTATGGCACGC
GCGCTGGGGCTCGCGCTGACCGGTGACAAGCTCGGCGCCGAGCAGGCCGAACAGTGGGGCCTGATCTGGCGTGCGGTCGA
CGACGACGCGCTCGTCGCGACAACCCGCCAGCTCGCCACCCAGCTCGCACAGCAGCCGACGCTCGCCATCGCATCGATCA
AGCAGTCGATGCGAGACAGCGTCACCAACACGCTCGATCAGCAGCTCGACGTGGAACGCGACCTGCAGCGCAAGCTCGGC
CAGTCGCACGACTATGCGGAAGGCGTGCAGGCTTTCATCGAGAAGCGCGCACCGCGCTTCGAGGGGCGTTGA

Upstream 100 bases:

>100_bases
TACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATCGCGATCGAACGCGTGTAACCCACGCGCCGCCCGCCTATAACGA
AGTCATGAAGGAGACACTGC

Downstream 100 bases:

>100_bases
CATGACGAATGCCACCGCCACGCTCGACCCCGATTCGCTCGCCCGCGCCACCGCGCAGGCCATGTACGACGCCGACGCCT
GCAGCCGTGCGTTCGGCATG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSYQAIQLDIDQAAGVATVTLNRPDKLNSFTRVMHRELQSALDEVEVAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFIKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDALVATTRQLATQLAQQPTLAIASIKQSMRDSVTNTLDQQLDVERDLQRKLG
QSHDYAEGVQAFIEKRAPRFEGR

Sequences:

>Translated_263_residues
MSYQAIQLDIDQAAGVATVTLNRPDKLNSFTRVMHRELQSALDEVEVAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFIKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDALVATTRQLATQLAQQPTLAIASIKQSMRDSVTNTLDQQLDVERDLQRKLG
QSHDYAEGVQAFIEKRAPRFEGR
>Mature_262_residues
SYQAIQLDIDQAAGVATVTLNRPDKLNSFTRVMHRELQSALDEVEVAGARALILTGAGRGFCAGQDLADLDFTPGASTDL
GTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFIKIGLVPDSGGTWFLPQRVGMARA
LGLALTGDKLGAEQAEQWGLIWRAVDDDALVATTRQLATQLAQQPTLAIASIKQSMRDSVTNTLDQQLDVERDLQRKLGQ
SHDYAEGVQAFIEKRAPRFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=33.1983805668016, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=29.5880149812734, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI31542718, Length=256, Percent_Identity=27.734375, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=29.4573643410853, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=28.4444444444444, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=28.4444444444444, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=209, Percent_Identity=26.7942583732057, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260275230, Length=209, Percent_Identity=26.7942583732057, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=209, Percent_Identity=26.7942583732057, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=25.6302521008403, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=25.6302521008403, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=28.6516853932584, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=25.6302521008403, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI25453481, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538573, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=243, Percent_Identity=23.4567901234568, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=27.6041666666667, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4757966, Length=251, Percent_Identity=23.1075697211155, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI51538581, Length=251, Percent_Identity=23.1075697211155, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI37594469, Length=232, Percent_Identity=26.7241379310345, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594471, Length=232, Percent_Identity=26.7241379310345, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI116235487, Length=158, Percent_Identity=29.1139240506329, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=57.7689243027888, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=35.2490421455939, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=35.4243542435424, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=28.3582089552239, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.7937743190661, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=28.6458333333333, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790281, Length=205, Percent_Identity=28.780487804878, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=34.9397590361446, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=30.9803921568627, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=28.6792452830189, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=27.3469387755102, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=227, Percent_Identity=28.6343612334802, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540306, Length=264, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=245, Percent_Identity=27.3469387755102, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=240, Percent_Identity=25.8333333333333, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=28.021978021978, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=28.021978021978, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=28.021978021978, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=27.2251308900524, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=30.6513409961686, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=30.6513409961686, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.5275590551181, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24654903, Length=263, Percent_Identity=26.9961977186312, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=25.9541984732824, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357171, Length=228, Percent_Identity=27.6315789473684, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28279; Mature: 28148

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSYQAIQLDIDQAAGVATVTLNRPDKLNSFTRVMHRELQSALDEVEVAGARALILTGAGR
CCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
GFCAGQDLADLDFTPGASTDLGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ACDLVFAARSSSFIQAFIKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCCCCHHHHHHHC
LIWRAVDDDALVATTRQLATQLAQQPTLAIASIKQSMRDSVTNTLDQQLDVERDLQRKLG
EEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
QSHDYAEGVQAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYQAIQLDIDQAAGVATVTLNRPDKLNSFTRVMHRELQSALDEVEVAGARALILTGAGR
CCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
GFCAGQDLADLDFTPGASTDLGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ACDLVFAARSSSFIQAFIKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCCCCHHHHHHHC
LIWRAVDDDALVATTRQLATQLAQQPTLAIASIKQSMRDSVTNTLDQQLDVERDLQRKLG
EEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
QSHDYAEGVQAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]