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The map label for this gene is mlaA [H]

Identifier: 116688441

GI number: 116688441

Start: 468641

End: 469603

Strand: Direct

Name: mlaA [H]

Synonym: Bcen2424_0417

Alternate gene names: 116688441

Gene position: 468641-469603 (Clockwise)

Preceding gene: 116688440

Following gene: 116688442

Centisome position: 13.45

GC content: 70.4

Gene sequence:

>963_bases
ATGCACACGATCCGCATCAGGCACGCCGCGCTGGCGATGGCGGCAGTCGCCGCGTTGAGCGGCTGCGCGACCGTGCAGAC
GCCGACCAAGGGCGATCCGCTCGAAGGCTTCAACCGGTCGATGTACAAGTTCAACGACACGGTCGATACGTACGCGCTGA
AGCCGGTCGCGAAGGGCTACCAGTACGTGGTGCCGCAGCCGGTGCGCGACAGCGTGACGAACTTCTTCTCGAACATCGGC
GACGTCTACATCGCGGCGAACAACATCGTGCAGCTGCGGATCGCGGACGGCGTCGGCGACATCATGCGCGTCGTGATCAA
CACGGTGTTCGGCGTCGGCGGCCTGTTCGACGTCGCGACGATCGCGAAGCTGCCGAAGCACACGGCCGACTTCGGGATCA
CGATGGGCCGCTACGGCGTGCCGTCGGGCCCGTATCTCGTGCTGCCGCTGCTCGGCCCGAGTACGCTGCGCGACACGGCC
GGCCTCGGCGTCGACTACGTCGGCAACCCGCTCACCTACGTGAAGCCGGACGGCCTGAGCTGGGGCCTGTTCGGCGTGAA
CCTGGTCAACACGCGCGCGAACCTGCTCGGCGCAGGCGACGTGCTGGATGCCGCGGCGCTCGACAAGTATTCGTTCGTGC
GCAACGCGTACCTGCAGCGTCGCCAGATGCTGATCAGCAACGCGCGCGGCGAGGCCGCCGCGACGTCGAGCAACGACGCG
CTGCCGAAGTACGATCTGCCGGAAGACGGCGCGGCACCGGCGGCAGCCGGCGCGGCCGGAACGGCGGGCGCGGCAGCGGT
GGGCGGCGCGGCGCCGGCGCCCGCATCGGGCGCGGCGGTGGCTGCGCCGGCCTCGGGCACGGCCGAAGCGCCGAATCCGG
CCAGCGAAACGAACGTGCCGGCGATGCAGGTGGCGCCGCCGTCGCCGGGCGGGTTCCGGTTCCCGAGCATCCGGCTGCAC
TGA

Upstream 100 bases:

>100_bases
GCCGCGCCGGCCCCGGTGGCGGTACCGGCGTCGGCGGTGTCCGGCGCGTCCGGCTCGGCGGCTCAATAACCACACGAGAG
AAAACAAGAGGGTAATGACC

Downstream 100 bases:

>100_bases
CGGACTTACATTCGTTACAGCCGGAAACGATTCAGTCGGTAGCGTAGGCGAACTTGCGCGTAAGCTACCTGCTCCAACCT
TCGCATCGACTCATTCATGC

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MHTIRIRHAALAMAAVAALSGCATVQTPTKGDPLEGFNRSMYKFNDTVDTYALKPVAKGYQYVVPQPVRDSVTNFFSNIG
DVYIAANNIVQLRIADGVGDIMRVVINTVFGVGGLFDVATIAKLPKHTADFGITMGRYGVPSGPYLVLPLLGPSTLRDTA
GLGVDYVGNPLTYVKPDGLSWGLFGVNLVNTRANLLGAGDVLDAAALDKYSFVRNAYLQRRQMLISNARGEAAATSSNDA
LPKYDLPEDGAAPAAAGAAGTAGAAAVGGAAPAPASGAAVAAPASGTAEAPNPASETNVPAMQVAPPSPGGFRFPSIRLH

Sequences:

>Translated_320_residues
MHTIRIRHAALAMAAVAALSGCATVQTPTKGDPLEGFNRSMYKFNDTVDTYALKPVAKGYQYVVPQPVRDSVTNFFSNIG
DVYIAANNIVQLRIADGVGDIMRVVINTVFGVGGLFDVATIAKLPKHTADFGITMGRYGVPSGPYLVLPLLGPSTLRDTA
GLGVDYVGNPLTYVKPDGLSWGLFGVNLVNTRANLLGAGDVLDAAALDKYSFVRNAYLQRRQMLISNARGEAAATSSNDA
LPKYDLPEDGAAPAAAGAAGTAGAAAVGGAAPAPASGAAVAAPASGTAEAPNPASETNVPAMQVAPPSPGGFRFPSIRLH
>Mature_320_residues
MHTIRIRHAALAMAAVAALSGCATVQTPTKGDPLEGFNRSMYKFNDTVDTYALKPVAKGYQYVVPQPVRDSVTNFFSNIG
DVYIAANNIVQLRIADGVGDIMRVVINTVFGVGGLFDVATIAKLPKHTADFGITMGRYGVPSGPYLVLPLLGPSTLRDTA
GLGVDYVGNPLTYVKPDGLSWGLFGVNLVNTRANLLGAGDVLDAAALDKYSFVRNAYLQRRQMLISNARGEAAATSSNDA
LPKYDLPEDGAAPAAAGAAGTAGAAAVGGAAPAPASGAAVAAPASGTAEAPNPASETNVPAMQVAPPSPGGFRFPSIRLH

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=231, Percent_Identity=37.6623376623377, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 32912; Mature: 32912

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTIRIRHAALAMAAVAALSGCATVQTPTKGDPLEGFNRSMYKFNDTVDTYALKPVAKGY
CCEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCC
QYVVPQPVRDSVTNFFSNIGDVYIAANNIVQLRIADGVGDIMRVVINTVFGVGGLFDVAT
EEECCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHH
IAKLPKHTADFGITMGRYGVPSGPYLVLPLLGPSTLRDTAGLGVDYVGNPLTYVKPDGLS
HHHCCCCHHHHCEECCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCE
WGLFGVNLVNTRANLLGAGDVLDAAALDKYSFVRNAYLQRRQMLISNARGEAAATSSNDA
ECEEEEEEECCHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LPKYDLPEDGAAPAAAGAAGTAGAAAVGGAAPAPASGAAVAAPASGTAEAPNPASETNVP
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
AMQVAPPSPGGFRFPSIRLH
EEEECCCCCCCCCCCCEEEC
>Mature Secondary Structure
MHTIRIRHAALAMAAVAALSGCATVQTPTKGDPLEGFNRSMYKFNDTVDTYALKPVAKGY
CCEEEHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCC
QYVVPQPVRDSVTNFFSNIGDVYIAANNIVQLRIADGVGDIMRVVINTVFGVGGLFDVAT
EEECCCCHHHHHHHHHHCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHH
IAKLPKHTADFGITMGRYGVPSGPYLVLPLLGPSTLRDTAGLGVDYVGNPLTYVKPDGLS
HHHCCCCHHHHCEECCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCEEEECCCCCE
WGLFGVNLVNTRANLLGAGDVLDAAALDKYSFVRNAYLQRRQMLISNARGEAAATSSNDA
ECEEEEEEECCHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LPKYDLPEDGAAPAAAGAAGTAGAAAVGGAAPAPASGAAVAAPASGTAEAPNPASETNVP
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
AMQVAPPSPGGFRFPSIRLH
EEEECCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]